2002
DOI: 10.1074/jbc.m200111200
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Interactions between Protein Kinase CK2 and Pin1

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Cited by 89 publications
(20 citation statements)
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“…CK2␣ and ␣Ј also show differences in phosphorylation (30 -32), cell cycle-dependent differences in localization (14), and differences in protein partners. Both ␣ and ␣Ј interact with nucleolin (33), yet only ␣ interacts with HSP90 (34,35), Pin-1 (36), and PP2A (21). Interestingly enough, the interaction between PP2A and CK2␣ was mapped to one of the few areas of non-identity between ␣ and ␣Ј in the N-terminal 330 amino acids (21).…”
Section: Ck2mentioning
confidence: 93%
“…CK2␣ and ␣Ј also show differences in phosphorylation (30 -32), cell cycle-dependent differences in localization (14), and differences in protein partners. Both ␣ and ␣Ј interact with nucleolin (33), yet only ␣ interacts with HSP90 (34,35), Pin-1 (36), and PP2A (21). Interestingly enough, the interaction between PP2A and CK2␣ was mapped to one of the few areas of non-identity between ␣ and ␣Ј in the N-terminal 330 amino acids (21).…”
Section: Ck2mentioning
confidence: 93%
“…Therefore, it is possible that a conformational change based on isomerization of this serineproline bond is also important in the regulation of E2 phosphorylation and degradation at specific stages of the viral life cycle. Notably, CK2 and Pin1 form a specific complex in mitosis (39).…”
Section: Discussionmentioning
confidence: 99%
“…To synchronize cells at the G 1 /S boundary, HeLa cells were incubated with media containing 2 mM thymidine for 16 h, washed with PBS, released into normal media for 12 h, and then incubated in media containing 2 mM thymidine for 15 h. S-phase cells were prepared by releasing G 1 /S cells into regular media for 3 h. Mitotic HeLa cells and COS-7 cells were prepared by incubating cells in media containing 100 ng/ml nocodazole for 16 h. Mitotic cells were then collected by mechanical shake-off. The Ba/F3 cells and p185 wt cells were synchronized at M-phase as described (33). Briefly, cells were incubated with 200 ng/ml nocodazole for 12 h. In the last 2 h of treatment, okadaic acid was added to a final concentration of 1 M. Under this condition, up to 75% of the p185 wt cells were arrested in M-phase, as determined by examining the mitotic index of DAPI-stained cells.…”
Section: Methodsmentioning
confidence: 99%