Interactions between red blood cells and a lethal, partly quaternized tertiary polyamine

In vitro study of the specific interaction between poly(2-dimethylamino ethylmethacrylate) based polymers with platelets and red blood cells

“…Although limited to standard ISO tests, these results have highlighted that PDMAEMAs did not cause hemolysis whatever their concentration (between 10 to 200 µg/mL) or Mw (10 to 40kDa) and irrespectively of the duration of incubation time (up to 120 min). These findings differ from the data reported earlier by Dubruel et al (Dubruel et al, 2003) and Moreau et al (Moreau et al, 2000) who demonstrated that PDMAEMA could induce a lysis of erythrocytes, depending on the Mw, concentration, and incubation time. This difference has been interpreted as a "buffering" role for plasma proteins, which were excluded from these former studies.…”

“…Because most blood proteins are anionic under physiological conditions, any circulating cationic macromolecule would most likely interact with plasma proteins. Through their rapid binding to polycations with formation of polyelectrolyte complexes, plasma proteins could significantly alter the affinity and selectivity of polycations for biological sites (Cerda-Cristerna et al, 2011;Domurado et al, 2001;Moreau et al, 2000).…”

Toxicity and Dose Response of Intra-Abdominally Administered Poly-<i>L</i>-α-Lysine and Poly-<i>L</i>-Glutamate for Postoperative Adhesion Protection

“…This is a well-known phenomenon and reported by other authors demonstrating discontinued toxicity of polycations when administered in certain sequences as regards other ionic substances [35] . As our previous dose of 200 mg/kg given intra-abdominally is high compared to the reported toxic intravenous dose of 2 mg/kg, we tried to establish a dose at which toxic symptoms occurred intra-abdominally, something that has never been shown before and which is crucial to the continued use of polypeptides as antiadhesive agents.…”

Dynamics of polyelectrolyte complex formation and stability when a polycation is progressively added to a polyanion under physico-chemical conditions modeling blood

“…4,5 On the one hand, the polycations, in excess, could be highly hemotoxic; [21][22][23] in addition, PEC particles, in an excess of polycations are going to be in contact with electrostatically charged red blood cells 24 and negatively charged proteins. In the case of injected PECs, based on our study, three fates can be considered: (1) stability with free circulation to the cells to be transfected provided with targeting can be exploited and macrophages do not intervene; (2) destabilization because of the ionic strength, a risk minimized if the PEC is formed in an iso-osmolar medium; and (3) exchange of complexed species because of the presence of a charged blood element having a high affinity for one of the polyplex components.…”