Interactions between the IBV Nucleocapsid Protein and RNA Sequences Specific for the 3′ end of the Genome

Collisson, Ellen W.; Williams, Anna K.; Chung, Shan-Ing; Zhou, Minglong

doi:10.1007/978-1-4615-1899-0_83

Cited by 4 publications

(3 citation statements)

References 7 publications

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“…Interestingly, there is a deletion of 22 nucleotides in the 5' terminus of 3'UTR ( Figure 1), which shows some similarity to the Chinese strains A2, BJ and SAIBb2. In the case of IBV, C-and N-terminal regions of the N protein (but not the central region) interact with the 3'end of the non-coding region of IBV genomic RNA [21]. This deletion suggests possible involvement of 3'UTR in replication, which may influence pathogenesis of the virus.…”

Section: Resultsmentioning

confidence: 99%

Complete Nucleotide Analysis of the Structural Genome of the Infectious Bronchitis Virus Strain Md27 Reveals its Mosaic Nature

Ammayappan

Vakharia

2009

Viruses

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Infectious bronchitis virus (IBV) causes highly contagious respiratory or urogenital tract diseases in chickens. The Maryland 27(Md27) strain was first isolated in 1976 from diseased chicken flocks in the Delmarva Peninsula region. To understand the genetic diversity and phylogenetic relationship of existing strains with Md27, the complete nucleotide sequence of the 3′end coding region (∼7.2 kb) of Md27 was determined and compared with other IBV strains and coronaviruses. It has the same S-3-M-5-N-3′ gene order, as is the case of other IBV strains. The spike gene of Md27 exhibits 97% identity with the SE17 strain. There are deletions at the spike gene, non-coding region between M and 5 genes, and at the 3′ untranslated region (UTR), which is different from Ark-like strains. Phylogenetic analysis and sequence alignments demonstrate that Md27 is a chimera containing different gene segments that are most closely related to the SE17, Conn and JMK strains. This current study provides evidence for genomic mutations and intergenic recombination that have taken place in the evolution of IBV strain Md27.

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Section: Resultsmentioning

confidence: 99%

Complete Nucleotide Analysis of the Structural Genome of the Infectious Bronchitis Virus Strain Md27 Reveals its Mosaic Nature

Ammayappan

Vakharia

2009

Viruses

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“…The discontinuous transcription method used by coronaviruses is very similar to that of the template switching that occurs during similarity-assisted or high frequency copy-choice RNA recombination (Sawicki and Sawicki 2005, Sawicki 2005). Recombination is a common phenomenon amongst coronaviruses (Wang et al 1993, Schaad and Baric 1994, Collisson et al 1995, Rest and Mindell 2003, Johnson et al 2005) and it may have greater evolutionary significance than the slow process of genetic drift. It provides a mechanism for the rapid formation of new viral strains with dramatically altered tropisms and pathogenicity, which can have a significant impact on host disease.…”

Section: Discussionmentioning

confidence: 99%

Genomic RNA sequence of feline coronavirus strain FCoV C1Je

Dye

Siddell

2007

Journal of Feline Medicine and Surgery

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This paper reports the first genomic RNA sequence of a field strain feline coronavirus (FCoV). Viral RNA was isolated at post mortem from the jejunum and liver of a cat with feline infectious peritonitis (FIP). A consensus sequence of the jejunum-derived genomic RNA (FCoV C1Je) was determined from overlapping cDNA fragments produced by reverse transcriptase polymerase chain reaction (RT-PCR) amplification. RT-PCR products were sequenced by a reiterative sequencing strategy and the genomic RNA termini were determined using a rapid amplification of cDNA ends PCR strategy. The FCoV C1Je genome was found to be 29,255 nucleotides in length, excluding the poly(A) tail. Comparison of the FCoV C1Je genomic RNA sequence with that of the laboratory strain FCoV FIP virus (FIPV) 79-1146 showed that both viruses have a similar genome organisation and predictions made for the open reading frames and cis-acting elements of the FIPV 79-1146 genome hold true for FCoV C1Je. In addition, the sequence of the 3′-proximal third of the liver derived genomic RNA (FCoV C1Li), which encompasses the structural and accessory protein genes of the virus, was also determined. Comparisons of the enteric (jejunum) and non-enteric (liver) derived viral RNA sequences revealed 100% nucleotide identity, a finding that questions the well accepted ‘internal mutation theory’ of FIPV pathogenicity.

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“…S1 protein forms a spike outside the virion and is important for infection and for the high variation frequency of IBV (Cavanagh, 1983;Mockett et al, 1984;Koch et al, 1990). N protein combines with the chromosome inside the virion and is thought to play an important role in sustaining the structure and replication of the chromosome (Baric and Nelson, 1988;Collisson et al, 1995;Zhou et al, 1996;Denison et al, 1999). S1 protein and N protein might play important roles in inducing immune protection in vaccinated or infected chicks.…”

mentioning

confidence: 99%

Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

Zhang¹,

Zhou²,

Chen³

et al. 2009

Vet. Med. - Czech

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ABSTRACT:The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocytochemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection.

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Interactions between the IBV Nucleocapsid Protein and RNA Sequences Specific for the 3′ end of the Genome

Cited by 4 publications

References 7 publications

Complete Nucleotide Analysis of the Structural Genome of the Infectious Bronchitis Virus Strain Md27 Reveals its Mosaic Nature

Complete Nucleotide Analysis of the Structural Genome of the Infectious Bronchitis Virus Strain Md27 Reveals its Mosaic Nature

Genomic RNA sequence of feline coronavirus strain FCoV C1Je

Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

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