Interactions of Biodegradable Poly([R]-3-hydroxy-10-undecenoate) with 1,2-Dioleoyl-sn-glycero-3-phosphocholine Lipid: A Monolayer Study

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable, biocompatible polyesters and very attractive candidates for biomedical applications as materials for tissue engineering. They have a hydrophobic character, but some are able to spread at the air-water interface to form monomolecularly thin films (Langmuir monolayers). This is a very convenient model to analyze PHA self-assembly in two dimensions and to study their molecular interactions with other amphiphilic compounds, which is very important considering compatibility between biomaterials and cell membranes. We used the Langmuir monolayer technique and Brewster angle microscopy to study the properties of poly([R]-3-hydroxy-10-undecenoate) (PHUE) films on the free water surface in various experimental conditions. Moreover, we investigated the interactions between the polymer and one of the main biomembrane components, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC). The addition of lipid to a polymer film does not change the monolayer phase behavior; however, the interactions between these two materials are repulsive and fall in two composition-dependent regimes. In summary, this is the first systematic study of the monolayer behavior of PHUE, thus forming a solid basis for a thorough understanding of material interactions, in particular in the context of biomaterials and implants.

Polymer–Lipid Hybrid Membranes as a Model Platform to Drive Membrane–Cytochrome c Interaction and Peroxidase-like Activity

“…Planar membranes on solid support are obtained by either substrate mediated vesicle fusion [28][29] or Langmuir Blodgett transfer method 19,28 , while their characterisation is achieved by a combination of surface methods including QCM-D, AFM and ellipsometry 12 . Due to the capability of lipids and copolymers to re-arrange the membrane architecture and generate domains, hybrid membranes represent ideal candidates for understanding protein-membrane interactions 30 . These domains facilitate the interaction with proteins, in a way similar to the so-called "lipid-rafts" found in cell membranes 15,30 .…”

“…Due to the capability of lipids and copolymers to re-arrange the membrane architecture and generate domains, hybrid membranes represent ideal candidates for understanding protein-membrane interactions 30 . These domains facilitate the interaction with proteins, in a way similar to the so-called "lipid-rafts" found in cell membranes 15,30 .…”

Recent Advances in Hybrid Biomimetic Polymer-Based Films: from Assembly to Applications

“…The capability of hybrid polymer-lipid membranes to undergo a rearrangement of their architectures in response to an external stimulus, e.g., upon contact with a biomolecule or a cell membrane, makes them applicable as a robust model for understanding membrane processes or investigating the biomolecule-membrane interactions. For example, polyhydroxylalkanoates (PHA) have been combined with DOPC for preparing monolayers at air-water interface as a model to analyze the molecular interactions between synthetic and biological membranes in terms of mixing/demixing properties [46]. Also, the incorporation of polymers into lipid-based systems can be a promising approach to improve the oral delivery of poorly water-soluble compounds, while the technique to incorporate the polymer plays an important role in affecting the bio-performance [368].…”

“…For each system, the assembly approach has been presented, in particular layer-by-layer, bottom-up, and top-down (grafting "from" and grafting "to", respectively), Langmuir-Blodgett and Langmuir-Schaefer methods, and some of the analytical methods commonly utilized for characterization of such films have been mentioned. The review has further exemplified their distinct properties and the properties-related applications for the development of biosensors [17][18][19][20][21][22][23][24][25][26][27][28][29][30], drug delivery platforms [16,[31][32][33][34][35][36][37][38][39][40][41][42][43], cell culture platforms [44,45], biomimicry [46,47], bioelectronic devices, e.g., [48], catalytic matrices [49][50][51], biomimetic lubricants First, we have described biomimetic hybrids based on polyelectrolytes and diverse biomacromolecules, natural polymers, and nanoparticles, followed by the presentation of solid-supported films based on polymer brushes produced either via bottom-up or top-down approach. Next, polymer-lipid tethered and cushioned composite films have been discussed with respect to their composition and properties.…”

“…For each system, the assembly approach has been presented, in particular layer-by-layer, bottom-up, and top-down (grafting "from" and grafting "to", respectively), Langmuir-Blodgett and Langmuir-Schaefer methods, and some of the analytical methods commonly utilized for characterization of such films have been mentioned. The review has further exemplified their distinct properties and the properties-related applications for the development of biosensors [17][18][19][20][21][22][23][24][25][26][27][28][29][30], drug delivery platforms [16,[31][32][33][34][35][36][37][38][39][40][41][42][43], cell culture platforms [44,45], biomimicry [46,47], bioelectronic Polymers 2020, 12, 1003 3 of 52 devices, e.g., [48], catalytic matrices [49][50][51], biomimetic lubricants [52,53], or anti-biofouling coatings, e.g., [14,54], among others, illustrated with the most interesting chosen examples based on the recent reports. Due to the aforementioned, this work has presented, in a comprehensive manner, multiple approaches to the synthesis, processing, and applications of hybrid biomimetic films based on various polymer species, which, in our opinion, would be interesting for both the specialists in the ...…”