Interactions of Chitosan-coated Green Synthesized Silver Nanoparticles
using Mentha spicata and Standard Antibiotics against Bacterial
Pathogens

Khan, Hamid; Andleeb, Saiqa; Nisar, Tayba; Latif, Zahid; Raja, Sadaf Azad; Awan, Uzma Azeem; Maqbool, Kiran; Khurshid, Sadia

doi:10.2174/1389201023666220405120914

Cited by 3 publications

(1 citation statement)

References 47 publications

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“…At the same time, the results we obtained when studying the effect of nanoceria on Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli, and Proteus vulgaris) are ambiguous. The same ambiguous reaction was obtained by other researchers when they studied a line of Gram-positive and Gram-negative microorganisms [84].…”

Section: Discussionsupporting

confidence: 81%

Antimicrobial Activity of Citrate-Coated Cerium Oxide Nanoparticles

Silina,

Ivanova,

Manturova

et al. 2024

Nanomaterials

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The purpose of this study was to investigate the antimicrobial activity of citrate-stabilized sols of cerium oxide nanoparticles at different concentrations via different microbiological methods and to compare the effect with the peroxidase activity of nanoceria for the subsequent development of a regeneration-stimulating medical and/or veterinary wound-healing product providing new types of antimicrobial action. The object of this study was cerium oxide nanoparticles synthesized from aqueous solutions of cerium (III) nitrate hexahydrate and citric acid (the size of the nanoparticles was 3–5 nm, and their aggregates were 60–130 nm). Nanoceria oxide sols with a wide range of concentrations (10−1–10−6 M) as well as powder (the dry substance) were used. Both bacterial and fungal strains (Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Proteus vulgaris, Candida albicans, Aspergillus brasielensis) were used for the microbiological studies. The antimicrobial activity of nanoceria was investigated across a wide range of concentrations using three methods sequentially; the antimicrobial activity was studied by examining diffusion into agar, the serial dilution method was used to detect the minimum inhibitory and bactericidal concentrations, and, finally, gas chromatography with mass-selective detection was performed to study the inhibition of E. coli’s growth. To study the redox activity of different concentrations of nanocerium, we studied the intensity of chemiluminescence in the oxidation reaction of luminol in the presence of hydrogen peroxide. As a result of this study’s use of the agar diffusion and serial dilution methods followed by sowing, no significant evidence of antimicrobial activity was found. At the same time, in the current study of antimicrobial activity against E. coli strains using gas chromatography with mass spectrometry, the ability of nanoceria to significantly inhibit the growth and reproduction of microorganisms after 24 h and, in particular, after 48 h of incubation at a wide range of concentrations, 10−2–10−5 M (48–95% reduction in the number of microbes with a significant dose-dependent effect) was determined as the optimum concentration. A reliable redox activity of nanoceria coated with citrate was established, increasing in proportion to the concentration, confirming the oxidative mechanism of the action of nanoceria. Thus, nanoceria have a dose-dependent bacteriostatic effect, which is most pronounced at concentrations of 10−2–10−3 M. Unlike the effects of classical antiseptics, the effect was manifested from 2 days and increased during the observation. To study the antimicrobial activity of nanomaterials, it is advisable not to use classical qualitative and semi-quantitative methods; rather, the employment of more accurate quantitative methods is advised, in particular, gas chromatography–mass spectrometry, during several days of incubation.

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Section: Discussionsupporting

confidence: 81%