Interactions of (−)-epigallocatechin-3-gallate with model lipid membranes

Comparing the Effects of Encapsulated and Non-Encapsulated Propolis Extracts on Model Lipid Membranes and Lactic Bacteria, with Emphasis on the Synergistic Effects of Its Various Compounds

“…In the case of EGCG, two T m peaks appear, instead of one, making the change in T m more complex to analyze. This phenomenon was described previously [ 60 ], and thus it will not be further commented here. As for t -FER and CAFF, they seem not to affect the T m of DPPC lipids’ main phase transition at least at the chosen pH.…”

“…The slight differences observed are likely due to a different insertion of compounds into the membranes, since MLVs were used in our study, as opposed to the ULVs used by Ota et al [ 23 ]. Though no differences were observed in T m for CHRY [ 64 ] and EGCG [ 22 , 60 ] compared to other studies, there seems to be a major difference in how QUER affected the phase transition of DPPC [ 62 , 63 ]. The results can be explained by the fact that though others incorporated QUER into the DPPC lipid membranes during the creation of liposomes [ 62 , 63 ], in our study, QUER was added to the already formed liposomes right before the DSC experiments.…”

“…In this case, the Mix was prepared at identical w compound(s) : w PL-90G mass ratios ( w CHRY : w CAFF : w t -FER : w QUER : w EGCG : w PL-90G = 1:1:1:1:1:100; final mass ratio of w Mix : w PL-90G = 1:20) and was compared to pure EGCG liposomes ( w EGCG : w PL-90G = 1:20) in order to observe any difference between the antioxidation potential of the pure compound (EGCG) and the Mix . EGCG was chosen since its antioxidant activity is well documented, as previously shown in our laboratory [ 60 ]. The results showed that the peroxidation of unsaturated lipids in the form of liposomes throughout the 24 h of exposure to UV light stayed the same in the case of Mix , whereas in the case of EGCG, statistically significant peroxidation of lipids occurred after 24 h ( Figure 10 ).…”