Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification

Zhang, Ruochen; Xu, Xiaomei; Chen, Chuan; Xing, Defeng; Shao, Bo; Liu, Wenzong; Wang, Aijie; Lee, Duu-Jong

doi:10.1016/j.watres.2018.06.053

Cited by 128 publications

(22 citation statements)

References 41 publications

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“…In these processes, chemolithotrophic sulfide-oxidizing denitrifying bacteria (SONB) (Thiobacillus sp., Sulfurimonas denitrificans, Beggiatoa sp., and Thiothrix sp.) and heterotrophic sulfide-oxidizing denitrifiers (Thauera-like taxa, Azoarcus, Pseudomonas, and Dechloromonas) cooperate in the processes of N removal [3,5,[9][10][11][12][13][14]. Moreover, sulfate reducing bacteria (SRB), such as Desulfobacteraceae, Desulfonema, and Thermotogaceae, in activated sludge (AS), which convert sulfate to sulfur, sulfide, and poly-S, could cooperate with SONB and enhance N removal by providing electron donors [3,8,15].…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, organic carbon, N and sulfate coexist in actual wastewater. Mixotrophic denitrification, which integrates the advantages of heterotrophic and autotrophic denitrification, is promising in removing N, and it can match the actual conditions of wastewater and reduce the requirement for carbon sources [6,11,20]. Furthermore, the A2/O process has temporal changes in redox conditions that create an environment favorable for sustaining sulfide for extended time periods, and previous researchers have reported sulfide-related corrosion in the A2/O process [14].…”

Section: Introductionmentioning

confidence: 99%

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Metagenomic insights into mixotrophic denitrification facilitated nitrogen removal in a full-scale A2/O wastewater treatment plant

2021

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Wastewater treatment plants (WWTPs) are important for pollutant removal from wastewater, elimination of point discharges of nutrients into the environment and water resource protection. The anaerobic/anoxic/oxic (A2/O) process is widely used in WWTPs for nitrogen removal, but the requirement for additional organics to ensure a suitable nitrogen removal efficiency makes this process costly and energy consuming. In this study, we report mixotrophic denitrification at a low COD (chemical oxygen demand)/TN (total nitrogen) ratio in a full-scale A2/O WWTP with relatively high sulfate in the inlet. Nitrogen and sulfur species analysis in different units of this A2/O WWTP showed that the internal sulfur cycle of sulfate reduction and reoxidation occurred and that the reduced sulfur species might contribute to denitrification. Microbial community analysis revealed that Thiobacillus, an autotrophic sulfur-oxidizing denitrifier, dominated the activated sludge bacterial community. Metagenomics data also supported the potential of sulfur-based denitrification when high levels of denitrification occurred, and sulfur oxidation and sulfate reduction genes coexisted in the activated sludge. Although most of the denitrification genes were affiliated with heterotrophic denitrifiers with high abundance, the narG and napA genes were mainly associated with autotrophic sulfur-oxidizing denitrifiers. The functional genes related to nitrogen removal were actively expressed even in the unit containing relatively highly reduced sulfur species, indicating that the mixotrophic denitrification process in A2/O could overcome not only a shortage of carbon sources but also the inhibition by reduced sulfur of nitrification and denitrification. Our results indicate that a mixotrophic denitrification process could be developed in full-scale WWTPs and reduce the requirement for additional carbon sources, which could endow WWTPs with more flexible and adaptable nitrogen removal.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metagenomic insights into mixotrophic denitrification facilitated nitrogen removal in a full-scale A2/O wastewater treatment plant

2021

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“…In addition, it was interesting to find that autotrophic denitrifiers, Thermomonas and Thiobacillus, instead of heterotrophic denitrifiers, dominated in FCS ( Fig 6C). The autotrophic denitrifiers, Thermomonas and Thiobacillus, could reduce nitrate by coupling Fe(II) and reduced sulfur oxidation, respectively [55,56]. However, denitrification efficiency of autotrophic denitrifiers was lower than heterotrophic denitrifiers due to their slow growth and the inadequate supply of electrons to reduce NO x -N. Therefore, we proposed that the biochemical processes in FCS might be less effective in removal of organics and N compared with LHS, resulting in the accumulation of organics and N in the urban river (Fig 2), which would then aggravate the accumulation of pollution in the river.…”

Section: Difference Of Bacterial Communities In An Urban Center and Smentioning

confidence: 99%

Insight into the nitrogen accumulation in urban center river from functional genes and bacterial community

Liu

Jiang

et al. 2020

PLoS ONE

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Along with urbanization, the intensified nitrogen pollution in urban rivers and the form of black-odor rivers has become one of the biggest concerns. Better understanding of the nitrogen transformations and microbial mechanisms occurring within urban rivers could help to manage their water quality. In this study, pollution characteristics, potential nitrogen removal rate, composition and function of bacterial community, and abundance of functional genes associated with nitrogen transformation were comparatively investigated in a typical urban river (FC) and a suburban river (LH). Compared with LH, FC was characterized by higher content of nutrients, lower potential nitrogen removal rate and lower abundance of functional genes associated with nitrogen transformation in both overlying water and sediment, especially in summer. Sediment dissolved organic matter characterized by excitation−emission matrix (EEM) showed that FC was more severely polluted by high nitrogen organic matter. Our results revealed that anammox was the main nitrogen removal pathway in both rivers and potential nitrogen removal rates decreased significantly in summer. Bacterial community analysis showed that the benthic communities were more severely influenced by the pollutant than aquatic ones in both rivers. Furthermore, the FC benthic community was dominated by anaerobic respiring, fermentative, sulfate reduction bacteria. Quantitatively, the denitrification rate showed a significant positive correlation with the abundance of denitrification genes, whilst the anammox rate was significantly negatively correlated with bacterial diversity. Meanwhile, NH 4 +-N had a significant negative correlation to both denitrification and anammox in sediment. Taken together, the results indicated that the increased nitrogen pollutants in an urban river altered nitrogen removal pathways and bacterial communities, which could in turn exacerbate the nitrogen pollution to this river.

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“…As a promising alternative of heterotrophic denitrication, autotrophic denitrication, using inorganic compounds including hydrogen gas, ferrous iron and reduced sulfur compounds as electron donor, is increasingly being studied in recent years. [17][18][19][20][21][22][23] Autotrophic denitrication especially hydrogen-based one (hydrogenotrophic denitrication) is desirable because of its nontoxicity and low biomass. 24 During the hydrogenotrophic denitrication process, hydrogen is used by hydrogenotrophic denitrifying bacteria as electron donor, and inorganic carbon, such as carbon dioxide and bicarbonate, is used as carbon source.…”

Section: Introductionmentioning

confidence: 99%

Bacteria-supported iron scraps for the removal of nitrate from low carbon-to-nitrogen ratio wastewater

Liu

Huang

et al. 2019

RSC Adv.

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Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification

Cited by 128 publications

References 41 publications

Metagenomic insights into mixotrophic denitrification facilitated nitrogen removal in a full-scale A2/O wastewater treatment plant

Metagenomic insights into mixotrophic denitrification facilitated nitrogen removal in a full-scale A2/O wastewater treatment plant

Insight into the nitrogen accumulation in urban center river from functional genes and bacterial community

Bacteria-supported iron scraps for the removal of nitrate from low carbon-to-nitrogen ratio wastewater

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