Interactions of histidine-containing test substances and extraction methods with the Ames mutagenicity test

Aeschbacher, H.U.; Finot, P. A.; Wolleb, U.

doi:10.1016/0165-1161(83)90223-6

Cited by 54 publications

(17 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…b. Feeding effects Feeding effects (e.g., release of histidine from test material) can give rise to increased revertant numbers in a Salmonella assay that are not due to genotoxic properties of the material [Aeschbacher et al, 1983;Gatehouse, 1987]. Further, test article and solvent interactions may have an impact on metabolism (e.g., inhibition of enzymatic activities) and there could be a non-specific reaction between test article and S9 proteins (i.e., no enzyme activity).…”

Section: A Non-physiological Conditionsmentioning

confidence: 99%

Follow‐up actions from positive results of in vitro genetic toxicity testing

Dearfield

Thybaud

Cimino

et al. 2010

Environ and Mol Mutagen

View full text Add to dashboard Cite

Appropriate follow-up actions and decisions are needed when evaluating and interpreting clear positive results obtained in the in vitro assays used in the initial genotoxicity screening battery (i.e., the battery of tests generally required by regulatory authorities) to assist in overall risk-based decision making concerning the potential effects of human exposure to the agent under test. Over the past few years, the International Life Sciences Institute (ILSI) Health and Environmental Sciences Institute (HESI) Project Committee on the Relevance and Follow-up of Positive Results in In Vitro Genetic Toxicity (IVGT) Testing developed a decision process flow chart to be applied in case of clear positive results in vitro. It provides for a variety of different possibilities and allows flexibility in choosing follow-up action(s), depending on the results obtained in the initial battery of assays and available information. The intent of the Review Subgroup was not to provide a prescriptive testing strategy, but rather to reinforce the concept of weighing the totality of the evidence. The Review Subgroup of the IVGT committee highlighted the importance of properly analyzing the existing data, and considering potential confounding factors (e.g., possible interactions with the test systems, presence of impurities, irrelevant metabolism), and chemical modes of action when analyzing and interpreting positive results in the in vitro genotoxicity assays and determining appropriate follow-up testing. The Review Subgroup also examined the characteristics, strengths, and limitations of each of the existing in vitro and in vivo genotoxicity assays to determine their usefulness in any follow-up testing.

show abstract

Section: A Non-physiological Conditionsmentioning

confidence: 99%

Follow‐up actions from positive results of in vitro genetic toxicity testing

Dearfield

Thybaud

Cimino

et al. 2010

Environ and Mol Mutagen

View full text Add to dashboard Cite

show abstract

“…The bacterial mutation assay is an easy method suitable for routine genetic toxicity screening for new chemicals, while data interpretation needs to be cautious as materials containing proteins, peptides or histidine can interfere the accuracy (Aeschbacher et al, 1983;Kirkland & Kim, 1995). In order to avoid false positive responses and overgrowth of background bacterial lawn, the genotoxicity potential of BPH was conducted with modified treatment and washing steps (Thompson et al, 2005) as well as a bacterial reverse mutation test (Ames test; using S. Typhimurium TA98 as a testing strain).…”

Section: Bacterial Reverses Mutation Assays For Bphmentioning

confidence: 99%

Composition characterization of Myctophids (Benthosema pterotum): Antioxidation and safety evaluations for Myctophids protein hydrolysates

Chai¹,

Chan²,

Li³

et al. 2012

Food Research International

View full text Add to dashboard Cite

“…Green et al (8) did report low levels of mutagens (three times the spontaneous rate) in ultrahigh-temperature pasteurized milk (135°C for 1 sec, then 20 min at 117°C). However, the histidine content of the milk samples (which were applied directly to the Ames test plates) was not taken into account and may have contributed to the apparent increase in numbers of revertants (9). Thus there is no substantial evidence that pasteurization processes promote mutagen formation.…”

Section: Pasteurizationmentioning

confidence: 99%

Mutagen formation during commercial processing of foods.

Krone¹,

Yeh²,

Iwaoka³

1986

Environ Health Perspect

View full text Add to dashboard Cite

Levels of bacterial mutagenicity 3-17 times above spontaneous are generated during commercial thermal processing (canning) of foods, particularly foods high in protein. The potential for other processing operations, including pasteurization, dehydration, and concentration, to produce substances active in the Ames Salmonella assay was also examined. Two heated fish model systems, canned salmon and fried sole, were established by extracting mutagen precursors from fish tissues with water. The model system studies suggest that the limiting reactants for mutagen formation differ from one food product to another, and that Maillard type browning reactions are involved in mutagen production. Bisulfite treatment was found to inhibit mutagen formation in modal systems and whole food products.Isolation and partial characterization of the mutagens in both fried and canned pink salmon showed that at least three distinct mutagens were present. These mutagens exhibited HPLC retention time patterns on C18, cyano, and amino columns different than the major mutagens present in other cooked and grilled meats and fish.

show abstract

Interactions of histidine-containing test substances and extraction methods with the Ames mutagenicity test

Cited by 54 publications

References 27 publications

Follow‐up actions from positive results of in vitro genetic toxicity testing

Follow‐up actions from positive results of in vitro genetic toxicity testing

Composition characterization of Myctophids (Benthosema pterotum): Antioxidation and safety evaluations for Myctophids protein hydrolysates

Mutagen formation during commercial processing of foods.

Contact Info

Product

Resources

About