Interactions of Human Colorectal Carcinoma Cells With Basement Membranes

Daneker, George W.; Piazza, Andrew J.; Steele, Glenn; Mercurio, Arthur M.

doi:10.1001/archsurg.1989.01410020053009

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…This protein is a well-characterized soluble lectin that recognizes the carbohydrate moieties on laminin.16·29 It is identical to Mac-2, a surface macrophage antigen, and L-34, a lectin characterized in murine melanoma cell line B-16 and reported to be involved in metastasis. [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30] idence that CBP-35 may be important for tumor cell func¬ tion is provided by reports showing involvement of lectins in neural crest cell migration, lymphocyte homing, and fertilization. At a molecular level, CBP-35 contains two distinct do¬ mains.…”

Section: Characteristics Of Colon Carcinoma Cell Linesmentioning

confidence: 99%

Carbohydrate-Binding Protein 35 Is the Major Cell-Surface Laminin-Binding Protein in Colon Carcinoma

Lee

Woo²,

Korzelius³

et al. 1991

Arch Surg

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The interaction of tumor cells with laminin is thought to be critical in invasion and metastasis. We found that an endogenous lectin, carbohydrate-binding protein 35 (CBP-35), is the major laminin-binding protein on human colon carcinoma cells and that its surface expression suggests involvement in metastasis. We identified CBP-35 by laminin-affinity chromatography and immunoblotting. Surface expression of CBP-35 on eight human colon carcinoma cell lines was compared by flow cytometry. Poorly differentiated cell lines and DLD-2, a signet-ring carcinoma cell line, expressed more surface CBP-35 than well-differentiated cell lines. Poorly differentiated cell lines and DLD-2 are characterized as aggressive cell lines because they adhere to and invade through reconstituted basement membrane significantly better than well-differentiated cell lines. These data suggest that CBP-35 is involved in tumor cell-basement membrane interactions and that an increase in CBP-35 surface expression may facilitate metastatic potential of colon carcinoma cells.

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