Interactions of JAZ Repressors with Anthocyanin Biosynthesis-Related Transcription Factors of Fragaria × ananassa

Abstract

Strawberry fruits are rich in flavonoids like proanthocyanidins and anthocyanins. Their biosynthesis and accumulation are controlled by the MYB-bHLH-WD40 (MBW) transcriptional complex, which is mainly formed by basic helix-loop-helix (bHLH) and MYB transcription factors (TFs). In Arabidopsis thaliana both bHLH and MYB TFs are repressed by JASMONATE ZIM-DOMAIN (JAZ) proteins, the key repressors of the jasmonate-signaling pathway. The aim of this research was the characterization of the FaJAZ1/8.1/9/10 proteins and molecular targets of signaling components and anthocyanin biosynthesis-related TFs of Fragaria × ananassa by protein–protein interactions. For this, domain compositions were studied by multiple alignments and phylogenetic analyses, while interactions were analyzed by yeast two-hybrid (Y2H) assays. We detected high conservation of FaJAZ proteins and jasmonate-signaling components, as well as FabHLHs and FaMYB10 TFs. Moreover, we report the F. × ananassa YABBY1 (FaYAB1) TF, which is related to anthocyanin biosynthesis in Arabidopsis, showed high conservation of functional domains. We demonstrated that FaJAZ repressors interacted with F. × ananassa NOVEL INTERACTOR OF JAZ (FaNINJA), FaMYC2, and JASMONATE ASSOCIATED MYC2-LIKE (FaJAM) proteins. Besides, transcription factors of MBW-complex like FabHLH3, FabHLH33, and FaMYB10, together with FaYAB1, were molecular targets of FaJAZ repressors, exhibiting specificity or redundancy of interaction depending on particular FaJAZ protein. Overall, these results suggest that interactions of jasmonate-signaling components are fully conserved, and anthocyanin biosynthesis might be regulated by JAZ repressors in F. × ananassa.

An integrated metabolome and transcriptome analysis of the Hibiscus syriacus L. petals reveal the molecular mechanisms of anthocyanin accumulation

“…Thus, the significant upregulation of anthocyanins on D2 stage of H. syriacus petals clearly correlates with the anthocyanin accumulation. This mechanism has been reported in Fragaria vesca L. x F. ananassa (Garrido-Bigotes et al, 2020). Other than JA signals, our results suggest that auxin signals can be another reason for the anthocyanin biosynthesis and resulting petal color.…”

“…JAZ’s (Jasmonate ZIM domain-containing protein) attachment with COI1 (coronatine-insensitive protein 1) allows it to detach from the MBW complex, which is then transcriptionally activated ( Garrido-Bigotes et al, 2020 ). Of 20 differentially expressed JAZ transcripts, 75% (15) showed reduced expression in D2 as compared to D1, which indicates JAZ’s are being degraded in D2.…”

Genome-Wide Identification of the TIFY Family in Salvia miltiorrhiza Reveals That SmJAZ3 Interacts With SmWD40-170, a Relevant Protein That Modulates Secondary Metabolism and Development

“…The JAs, SCF COI 1 receptor complex, JAZ repressors, and TFs are all involved in JA signal transduction ( Xie et al, 1998 ; Xu et al, 2002 ; Boter et al, 2004 ; Thines et al, 2007 ; Chini et al, 2010 ). JAZ is an important juncture that represses responses to JA by interacting with bHLH-TFs (MYC2, MYC3, MYC4, MYC5, GL3, EGL3, and TT8) and R2R3-MYB TFs (PAP, GL1, MYB21, and MYB24) ( Fernández-Calvo et al, 2011 ; Song et al, 2011 , 2013 ; Fonseca et al, 2014 ; Qi et al, 2014 ; Garrido-Bigotes et al, 2020 ). Certain JAZ proteins and their interaction partners have been identified in S. miltiorrhiza .…”

Characterization of YABBY transcription factors in Osmanthus fragrans and functional analysis of OfYABBY12 in floral scent formation and leaf morphology

“…As important model plants, the functions of the A. thaliana and S. lycopersicum YABBY genes have been extensively examined. These genes mainly play important roles in plant growth and development, particularly in leaf growth, fruit development, floral organ formation, and the synthesis of plant secondary metabolites [14,27,49,50]. In addition, subcellular localization analysis showed that 35 S::GFP-OfY-ABBY3/5/7/8/12/13 fusion proteins were mainly located in the nucleus, suggesting that these proteins have diverse functions in the nucleus.…”