Interactions of KLVFF‐PEG Peptide Conjugate with Fibrinogen in Neutral Aqueous Solutions

Abstract

In this work we report on the interaction of KLVFF-PEG with fibrinogen (Fbg) in neutral aqueous solutions at 20 degrees C, for particular ratios of KLVFF-PEG to Fbg concentration, Delta = c(KLVFF-PEG)/c(Fbg). Our results show the formation of Fbg/KLVFF-PEG complexes for Delta > 0, such that there is not an extended network of complexes throughout the solution. In addition, cleaved protein and Fbg dimers are identified in the solution for Delta >or= 0. There is a dramatic change in the tertiary structure of the Fbg upon KLVFF-PEG binding, although the KLVFF-PEG binds to the Fbg without affecting the secondary structure elements of the glycoprotein.

Metformin Mediated PD‐L1 Downregulation in Combination with Photodynamic‐Immunotherapy for Treatment of Breast Cancer

“…The Ce6 signal of MA‐pepa‐Ce6 NPs group was very weak and mostly around the blood vessels (Figure 3D), suggesting that although MA‐pepa‐Ce6 NPs could reach the tumor site via EPR effects, they were easily pumped out into the systemic circulation due to the high interstitial fluid pressure in tumor site. [ 16 ] Conversely, the Ce6 signal of MA‐pepA‐Ce6 NPs was stronger than MA‐pepa‐Ce6 NPs and far away from the intratumor vessels, further revealing that the binding between VRGDK‐Ce6 and α v β 3 integrin receptor expressed on tumor cells enhanced the accumulation and penetration efficiency of MA‐pepA‐Ce6 NPs.…”

Linear Chimeric Triblock Molecules Self‐Assembled Micelles with Controllably Transformable Property to Enhance Tumor Retention for Chemo‐Photodynamic Therapy of Breast Cancer

“…This problem can be resolved better by nanomedicines staying in shape one, such as spheres, to circulate and distribute in the body, and transforming into another shape, such as fibers, to facilitate the retention inside tumor. Phe‐Phe‐Val‐Leu‐Lys (FFVLK) peptide, derived from β‐amyloid, tends to form a β‐sheet structure relying on extensive intermolecular hydrogen bonds that enable fibers formation . However, after introducing hydrophobic heads and hydrophilic tail in FFVLK peptide, the resultant linear chimeric molecules form micelles when rapidly dissolved in aqueous solvents.…”

Metformin‐Mediated Immunosuppressive Microenvironment Remodeling in Combination with Chemotherapy via a Spatial‐Specific Multi‐Responsive Carrier‐Free Self‐Assembled Nanoparticle

“…As shown in Figure 4C, the signal of NMR NPs was weak and mainly near blood vessels, indicating that NMR NPs could be easily pumped out into the systemic circulation under the high interstitial fluid pressure at tumor site. [ 14 ] On the contrary, the fluorescence signal of MR NPs was strong and distributed evenly throughout the tumor, further confirming the stronger penetration ability and tumor retention capacity. This might because the exposure of VRGDK‐SS‐SN38 further targeted to the α v β 3 integrin receptor expressed on tumor cells, and thus led to a spatial‐specific release and reinforced accumulation.…”