Interactions of prostaglandins with subpopulations of ovine luteal cells. II. Inhibitory effects of PGF2α and protection by PGE2

Fitz, T. A.; Mock, Édward J.; Mayan, M.H.; Niswender, G. D.

doi:10.1016/0090-6980(84)90120-5

Cited by 69 publications

(22 citation statements)

References 37 publications

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“…The results of present study of luteinized granulosa cells concur with the general view that prostaglandins of the F series are physiological agents that irreversibly interfere with luteal function [3]. It should be noted that corpora lutea of most species are believed to have both thecal and granulosa cell antecedents [3,20]. However, we believe that the effects of PGF 2 ,, on porcine granulosa cells are indicative of the role of PGF2a in luteolysis in the pig.…”

Section: Discussionsupporting

confidence: 91%

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Prostaglandins Alter the Abundance of Messenger Ribonucleic Acid for Steroidogenic Enzymes in Cultured Porcine Granulosa Cells1

Juorio

Murphy

1993

Biology of Reproduction

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The effects of prostaglandin F2 alpha (PGF2 alpha) and prostaglandin E2 (PGE2) on the accumulation of progesterone and mRNA for the steroidogenic enzymes 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD) and cytochrome P450 side-chain cleavage (P450scc) were determined in luteinized granulosa cells. These cells were aspirated from 3-5-mm follicles of prepubertal pigs and cultured 48 h with 10% serum and then 48 h under serum-free conditions. Cells were then incubated with medium (control), ovine LH (20 ng/ml), (bu)2cAMP (0.5 mM) and doses of PGF2 alpha (0.08-2.0 micrograms/ml) or PGE2 (0.1-1.0 micrograms/ml), or combinations of PGF2 alpha (0.1-1.0 micrograms/ml) or PGE2 (0.1-1.0 micrograms/ml) with and without 20 ng/ml LH. Cultures were terminated after 12 h of incubation, and the abundance of mRNA for 3 beta-HSD and P450scc was determined by hybridization with specific cDNA probes. Parallel cultures were terminated at 24 h for analysis of progesterone in the medium by RIA. The results demonstrated that LH and (bu)2cAMP elevated progesterone by 3- to 6-fold and the accumulation of mRNA for 3 beta-HSD by 1.5 to 3.5 or 4-fold and for P450scc by 2-4-fold. PGF2 alpha reduced the basal level of progesterone accumulation, as well as the steady-state concentrations of mRNA for both 3 beta-HSD and P450scc. PGF2 alpha interfered with LH and (bu)2cAMP induction of 3 beta-HSD and P450scc message, and this interference was dependent on the PGF2 alpha dose employed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionsupporting

confidence: 91%

“…A role in protecting luteal cells from PGF 2 -induced luteolysis has been ascribed to PGE 2 in both sheep [20] and pigs [21]. PGE 2 functions by stimulation of the cAMP pathway [22].…”

Section: Introductionmentioning

confidence: 98%

Prostaglandins Alter the Abundance of Messenger Ribonucleic Acid for Steroidogenic Enzymes in Cultured Porcine Granulosa Cells1

Juorio

Murphy

1993

Biology of Reproduction

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“…Alternatively, the antiluteolytic effect of PGE 2 may involve the participation of other synergistic signals, such as an ovine trophoblastic protein, that are missing from this system. On the surface, these data contrast with those of earlier studies in which PGE 2 appeared to protect against the inhibitory effects of PGF 2 , [40]. On closer scrutiny, however, the conflicting data are seen to have been generated under vastly different conditions: previous investigations did not involve substrate supplementation and were limited to only short-term incubations ( 18 h).…”

Section: *contrasting

confidence: 78%

Effects of Individual and Combined Treatment with Prostaglandins E2 and F2α on Progesterone Secretion by Ovine Luteal Cells Supplemented with Homologous Serum Lipoproteins in Vitro1

Fitz

Marr

Contois

et al. 1993

Biology of Reproduction

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Prostaglandin F2 alpha (PGF2 alpha) is a potent luteolysin, whereas prostaglandin E2 (PGE2) is generally luteotropic in vivo. To establish a model system for investigations of the mechanisms involved in these actions, we examined the effects of individual and combined treatment with PGE2 and PGF2 alpha on basal and ovine LH-stimulated progesterone secretion during long-term incubations conducted with and without supplemental homologous low-density lipoprotein (LDL) as substrate. Effects of both PGF2 and PGF2 alpha were concentration- and time-dependent and were further influenced by the presence of LDL and/or LH in medium. Neither of the prostaglandins exerted any significant effect before 48 h in culture, but distinctly different patterns of response to PGE2 and PGF2 alpha emerged thereafter. Low, but not high, concentrations of PGE2 increased progesterone secretion in the absence of LH, whereas PGF2 alpha (alone and in combination with PGE2) inhibited progesterone production in all medium formulations. The transcriptional inhibitor actinomycin effectively blocked the actions of PGF2 alpha, but had no effect on response to LH or PGE2. These data demonstrate that both the putative luteotropic actions of PGE2 and the potent, luteolytic effects of PGF2 alpha in vivo can be reproduced in long-term cultures of ovine luteal cells in vitro, and they suggest that the mechanism of PGF2 alpha-induced luteolysis may involve new protein synthesis.

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“…PGF-2a has been reported to inhibit progesterone secretion and to have a direct cytotoxic effect on large luteal cells in vitro (Fitz, Mock, Mayan & Niswender, 1984b). This response was not a nonspecific toxicity of the PGF-2a because it could be prevented by coculturing with PGE-2.…”

Section: Introductionmentioning

confidence: 88%

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

Schwall¹,

Sawyer²,

Niswender³

1986

Reproduction

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Interactions of prostaglandins with subpopulations of ovine luteal cells. II. Inhibitory effects of PGF2α and protection by PGE2

Cited by 69 publications

References 37 publications

Prostaglandins Alter the Abundance of Messenger Ribonucleic Acid for Steroidogenic Enzymes in Cultured Porcine Granulosa Cells1

Prostaglandins Alter the Abundance of Messenger Ribonucleic Acid for Steroidogenic Enzymes in Cultured Porcine Granulosa Cells1

Effects of Individual and Combined Treatment with Prostaglandins E2 and F2α on Progesterone Secretion by Ovine Luteal Cells Supplemented with Homologous Serum Lipoproteins in Vitro1

Differential regulation by LH and prostaglandins of steroidogenesis in small and large luteal cells of the ewe

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