Interactions of radiolabeled tuftsin with human neutrophils

Nair, R. M. G.; Ponce, Bruce; Fudenberg, H. Hugh

doi:10.1016/0161-5890(78)90125-6

Cited by 48 publications

(7 citation statements)

References 30 publications

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“…Tuftsin has been shown to enhance bactericidal activity in addition to phagocytosis. Its biological activities appear to be mediated via specific tuftsin receptors which have been found on mammalian macrophages, monocytes and granulocytes (Constantopoulos & Najjar 1972;Nair, Ponce & Fudenberg 1978). In addition, tuftsin possesses chemotactic, migration-enhancing and mitogenic properties for mammalian leucocytes, and has been shown to enhance their antitumour activity in vitro as well as in vivo (Nishioka 1979;Nishioka, Amoscato & Babcock 1981).…”

Section: Discussionmentioning

confidence: 99%

Optimising of culture conditions and stimulation of head kidney macrophages from Atlantic cod, Gadus morhua L.

Steiro

Johansen

Gildberg

et al. 1998

Journal of Fish Diseases

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The cultivation of Atlantic cod, Gadus morhua L., head kidney macrophages was optimal at 4±6°C. The viability of cells in culture was examined by the trypan blue exclusion test 2, 5 and 7 days after seeding. Simultaneously, the number of adherent cells at the various times was checked. The morphology of the cells was also evaluated during the cultivation period. Enhanced respiratory burst activities were demonstrated in macrophages after stimulation with known immunostimulants like lipopolysaccharide, tuftsin and an acid peptide fraction previously shown to stimulate Atlantic salmon leucocytes efficiently. Generally, the Atlantic cod macrophages seemed to respond to a lesser extent compared with macrophages obtained from both mammalian and other fish species. Figure 5 Reduction of nitroblue tetrazolium in cod macrophages stimulated with tuftsin (5 mg mL ±1 ), chitosan (25 mg mL ±1 ) and APF-2 (5 mg mL ±1 ) at 4, 8 and 12°C for 2, 5 and 7 days. The results are expressed as OD 620´1 0 5 cell ±1 and data represent four readings (SD) from three individual cod.

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Section: Discussionmentioning

confidence: 99%

Optimising of culture conditions and stimulation of head kidney macrophages from Atlantic cod, Gadus morhua L.

Steiro

Johansen

Gildberg

et al. 1998

Journal of Fish Diseases

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“…The reSults obtained in our laboratorY [4-9, 32-34, 38-40] by Martinez et al, [25,26] and Tzehoval et al [49] emphasize the highly specific nature of the functional activity of tuftsin. In fact, the very phagocytic cells that tuftsin stimulates, the blood neutrophils and body macrophages, alone of all blood cells show specific receptors to tuftsin [27,48].…”

Section: Discussionmentioning

confidence: 99%

Klinische und physiologische aspekte von Tuftsin-Mangelsyndromen mit gest�rter Phagocytose

Najjar

1979

Klin Wochenschr

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The tetrapeptide tuftsin (Thr-Lys-Pro-Arg) stimulates phagocytosis by blood neutrophilic granulocytes and tissue macrophages in a highly specific manner. Tuftsin is cleaved off the carrier gamma-globulin molecule as the free active form by two enzymes. One of these is in the spleen and the other on the outer membrane of the phagocyte. Congenital tuftsin deficiency usually arises when the peptide is mutated to an inactive peptide. The acquired type occurs if the spleen function is curtailed by removal or disease. Tuftsin deficiency is manifested by severe recurrent infections involving primarily the skin, lymph nodes and lungs. Therapy is limited to gamma-globulin injection along with appropriate chemotherapy.

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“…A second approach, employed by Fudenburg and colleages (47), was the chemical introduction of a radiolabelled tracer into the C-terminal portion of tuftsin. In this manner tuftsin-14 C-methyl ester and 1251-tuftsyl-tyrosine were prepared and used for binding studies on circulatory cells.…”

Section: Periphary Ce//smentioning

confidence: 99%

“…However, only binding displayed by 1251-tuftsyl-tyrosine could be considered statistically significant (47). However, only binding displayed by 1251-tuftsyl-tyrosine could be considered statistically significant (47).…”

Section: Periphary Ce//smentioning

confidence: 99%

Tuftsin, Thr-Lys-Pro-Arg

Fridkin

Gottlieb

1981

Immunologically Active Peptides

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Tuftsin, a natural occurring tetrapeptide, has been found to exhibit several biological activities connected with immune system function. Although little is known about tuftsin's 'biogenesis', much information has been gleaned about its structure-function relationships, which have shown that several features of the molecule are essential for expression offull biological activity. Furthermore, specific receptor sites for tuftsin have been found to exist exclusively on phagocytic cells. Research indicates that tuftsin binding to target cells effect intracellular calcium and cyclic nucleotide levels. Implication of these facts on tuftsin's mode of action are discussed.Basic peptidic segments resembling tuftsin are found in a variety of regulatory peptides. Questions are, therefore, raised as to the biospecificity and cross-reactivity of these sequences. Substance P, one such peptide, which binds with and activates tuftsin receptors, is described.In light of tuftsin's therapeutic potential, assays for its determination have been introduced. When applied to analyze human blood serum of normal as well as of various pathological origins, direct correlation was found between tuftsin levels and susceptibility to bacterial infections. Molecular and Cellular Biochemistry 41,73-97 (1981). 0300 8177/81 0041 0073/$05.00.

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Interactions of radiolabeled tuftsin with human neutrophils

Cited by 48 publications

References 30 publications

Optimising of culture conditions and stimulation of head kidney macrophages from Atlantic cod, Gadus morhua L.

Optimising of culture conditions and stimulation of head kidney macrophages from Atlantic cod, Gadus morhua L.

Klinische und physiologische aspekte von Tuftsin-Mangelsyndromen mit gest�rter Phagocytose

Tuftsin, Thr-Lys-Pro-Arg

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