Interactions of reactive sulfur species with metalloproteins

Domán, Andrea; Dóka, Éva; Garai, Dorottya; Bogdándi, Virág; Balla, György; Balla, József

doi:10.1016/j.redox.2023.102617

Cited by 35 publications

(23 citation statements)

References 253 publications

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“…Alternatively, hydrogen sulfide can be generated via non‐enzymatic processes that rely on glutathione 24,243 . It is important to note that hydrogen sulfide exerts its biological effects through interactions with nitric oxide and metalloproteins, including haemoglobin 244,245 . Methaemoglobin, in the presence of NADPH and flavin oxidoreductases, can bind to and oxidize hydrogen sulfide, resulting in the formation of thiosulfate and polysulfides, which are important for hydrogen sulfide‐mediated signaling 241,246 .…”

Section: The Map Of Erythrocyte Metabolismmentioning

confidence: 99%

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

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Our aim is to present an updated overview of the erythrocyte metabolism highlighting its richness and complexity. We have manually collected and connected the available biochemical pathways and integrated them into a functional metabolic map. The focus of this map is on the main biochemical pathways consisting of glycolysis, the pentose phosphate pathway, redox metabolism, oxygen metabolism, purine/nucleoside metabolism, and membrane transport. Other recently emerging pathways are also curated, like the methionine salvage pathway, the glyoxalase system, carnitine metabolism, and the lands cycle, as well as remnants of the carboxylic acid metabolism. An additional goal of this review is to present the dynamics of erythrocyte metabolism, providing key numbers used to perform basic quantitative analyses. By synthesizing experimental and computational data, we conclude that glycolysis, pentose phosphate pathway, and redox metabolism are the foundations of erythrocyte metabolism. Additionally, the erythrocyte can sense oxygen levels and oxidative stress adjusting its mechanics, metabolism, and function. In conclusion, fine‐tuning of erythrocyte metabolism controls one of the most important biological processes, that is, oxygen loading, transport, and delivery.

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Section: The Map Of Erythrocyte Metabolismmentioning

confidence: 99%

Erythrocyte metabolism

Chatzinikolaou,

Margaritelis,

Paschalis

et al. 2024

Acta Physiologica

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“…The abnormal accumulation of specific ions in cells can cause irreversible damage or trigger cell death. 109 This has led to the development of ion interference therapy (IIT) for treating tumors, using nanomaterials for better ion delivery and biocompatibility compared to molecular carriers. 110,111 Zheng et al made a pioneering contribution by documenting the utilization of biodegradable Ca 2+ nanomodulators (CaNMs) in cancer immunotherapy (Fig.…”

Section: Ion-interference Therapymentioning

confidence: 99%

Section: Collaborative Utilization Of Nanomedicine In Inducing Antitu...mentioning

confidence: 99%

Nanomedicine-induced cell pyroptosis to enhance antitumor immunotherapy

Ge,

Zhang,

Zhao

et al. 2024

J. Mater. Chem. B

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“…In striking contrast, disulfides and peroxides do not react with HlyU nor do they impact DNA binding. We found that the exogenous treatment of V. cholerae cells with either sulfide (Na 2 S) or hydrogen peroxide (H 2 O 2 ) attenuates HlyU-dependent activation of hlyA , downregulating hlyA transcription likely through transcriptional silencing by the nucleoprotein H-NS (63). Quantitative RSS metabolite profiling experiments reveal that both treatments result in an increase in the levels of inorganic persulfides thus reconciling the in vitro and in vivo results.…”

Section: Introductionmentioning

confidence: 99%

Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation inVibrio cholerae

Diez

Antelo

Dalia

et al. 2023

Preprint

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In vertebrates, host's immune system and resident commensal bacteria deploy a range of highly reactive small molecules that provide a barrier against microbial pathogens, including those known to induce oxidative and sulfide stress. Gut pathogens, such as Vibrio cholerae, sense and respond to these stressors by modulating the expression of exotoxins that are crucial for colonization. Here, we employ mass-spectrometry-based profiling, metabolomics, expression assays and biophysical approaches to show that transcriptional activation of the hemolysin gene hlyA in V. cholerae is regulated by intracellular RSS (reactive sulfur species, specifically sulfane sulfur). We first report a sequence similarity network analysis of the arsenic repressor (ArsR) superfamily of transcriptional regulators where RSS and ROS (reactive oxygen species) sensors segregate into distinct clusters. We show that HlyU, transcriptional activator of hlyA in V. cholerae, belongs to the RSS-sensing cluster and readily reacts with organic persulfides, showing no reactivity and remaining DNA-bound following treatment with various ROS in vitro, including H2O2. Surprisingly, in V. cholerae cell cultures both sulfide and peroxide treatment downregulate HlyU-dependent transcriptional activation of hlyA. However, RSS metabolite profiling showed that both sulfide and peroxide treatment raise the endogenous inorganic sulfide and disulfide levels to a similar extent, accounting for this crosstalk, and confirming that V. cholerae attenuates HlyU-mediated activation of hlyA in a specific response to intracellular RSS. These findings provide new evidence that gut pathogens may harness RSS-sensing as an evolutionary adaptation that allows them to overcome the gut inflammatory response by modulating the expression of exotoxins.

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Interactions of reactive sulfur species with metalloproteins

Cited by 35 publications

References 253 publications

Erythrocyte metabolism

Erythrocyte metabolism

Nanomedicine-induced cell pyroptosis to enhance antitumor immunotherapy

Increased intracellular persulfide levels attenuate HlyU-mediated hemolysin transcriptional activation inVibrio cholerae

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