2020
DOI: 10.1093/nar/gkaa826
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Interactions of replication initiator RctB with single- and double-stranded DNA in origin opening of Vibrio cholerae chromosome 2

Abstract: Studies of bacterial chromosomes and plasmids indicate that their replication initiator proteins bind to origins of replication at many double-stranded sites and also at AT-rich regions where single-stranded DNA is exposed during origin opening. Single-strand binding apparently promotes origin opening by stabilizing an open structure, but how the initiator participates in this process and the contributions of the several binding sites remain unclear. Here, we show that the initiator protein of Vibrio cholerae … Show more

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Cited by 15 publications
(17 citation statements)
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“…DNA distortion is induced by binding of the plasmid initiator to an iteron array ( 37 , 38 ). It is likely that binding of RctB to the six-iteron array of ori2 catalyzes the initiation of ori2 replication by mediating a change in DNA conformation ( 7 ) without requiring RctB cooperative binding to DNA.…”
Section: Discussionmentioning
confidence: 99%
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“…DNA distortion is induced by binding of the plasmid initiator to an iteron array ( 37 , 38 ). It is likely that binding of RctB to the six-iteron array of ori2 catalyzes the initiation of ori2 replication by mediating a change in DNA conformation ( 7 ) without requiring RctB cooperative binding to DNA.…”
Section: Discussionmentioning
confidence: 99%
“…Chr2: RctB binding to 39m is inhibited which releases ori2 . Binding of RctB to methylated iterons causes opening of the DNA unwinding element (DUE) and oligomerization of RctB onto single-stranded DNA (green arrows) ( 7 ). ( B ) Acquisition of a unique domain IV by the initiator RctB to coordinate replication of Chr1 and Chr2.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Integration host factor (IHF) induces DNA to bend backwards in the loop-back model, bringing the DUE close to the DnaA protein bound to the DnaA box and thus simultaneously facilitating protein binding to double- and single-stranded DNA sequences. This mechanism has been identified in E. coli [47], and a similar mechanism might be also found in Helicobacter pylori [48] with a bipartite oriC and V. cholerae chromosome 2 whose replication initiator requires RctB protein other than DnaA protein [49].…”
Section: Introductionmentioning
confidence: 72%
“…Some sequence specificity in the interaction with ssDNA was previously shown for S. cerevisiae ORC proteins complex [ 16 ]. Also, for other DNA replication initiators: bacterial [ 4 , 5 , 23 ], plasmid [ 6 ] and viral [ 24 ], it was shown that they bind to ssDNA in a sequence-specific manner. Usually the T-rich strand is bound by replication initiator as it is observed for Ap Orc1 (Fig.…”
Section: Resultsmentioning
confidence: 99%