A 60-days feeding trial was conducted to study the dietary energy requirement of Pangasianodon hypophthalmus (Sauvage1878) juveniles reared either at ambient (24.5°C) or higher temperature (32°C) and fed with one of the four diets having dietary energy content of 342 Kcal. 100 g -1 (T1), 379 Kcal. 100 g -1 (T2), 428.5 Kcal. 100 g -1 (T3) and 448.6 Kcal. 100 g -1 (T4). Juveniles of P. hypophthalmus with amean weight 4.27±0.12 to 4.40±0.10 g was stocked in 24 uniform size plastic rectangular tanks in triplicates with 10 fish per tank following a 2x4 factorial design. Growth performance and nutrient utilization of P. hypophthalmus were higher at the dietary energy content of 428.5 Kcal. 100 g -1 (T3) at both the temperatures. The body composition of P. hypophthalmus did not differ significantly (P>0.05) as the energy content of the diet increase. However, the tissue lipid content was significantly varied (P<0.05) at different temperature and energy levels. Based on the second order polynomial regression equation the dietary energy requirement of P. hypophthalmus was recorded to be 410 Kcal. 100 g -1 and 402.5 Kcal. 100 g -1 at 32°C and at ambient temperature(24.5°C), respectively.