Interactions of Wild-Type and a Cellulose-Minus Mutant ofAgrobacterium tumefacienswith Tobacco Mesophyll and Tobacco Tissue Culture Cells

Time required for tumor induction by Agrobacterium tumefaciens

“…The mutant strain Ce-12 used in these experiments was described previously (6). It does not form cellulose fibrils in tobacco leaves, tobacco callus cultures, tobacco or carrot suspension cultures, or bacteriological media with or without soytone (2,6). This mutant binds only loosely to the plant cell surface.…”

Signal exchange in plant-microbe interactions.

“…Microfibril synthesis. Production of cellulose microfibrils by A. tumefaciens in response to attachment to carrot cell suspension cultures (72,168,171,190) and protoplasts (172), or to tobacco leaf mesophyll or suspension culture cells and callus (116), has been reported. Cellulose microfibril production is not required for virulence (168).…”

“…with the plant cell receptor(s) induces the production of fibrils to further anchor the bacterium to the plant cell surface. Attachment generally occurs within 30 to 90 min, and the fibril production increases with time (72,168,171).…”

“…The number of fibrils formed by A. tumefaciens on tobacco is dependent on the type of tissue utilized (72). Strain A6, when attached to tobacco suspension culture cells, synthesizes greater numbers of cellulose fibrils than after its attachment to tobacco callus cells.…”