Interactive effects of LPS and dentine matrix proteins on human dental pulp stem cells

Widbiller, Matthias; Eidt, Andreas; Wölflick, M.; Lindner, Sophia R.; Schweikl, Helmut; Hiller, Karl‐Anton; Buchalla, Wolfgang; Galler, Kerstin M.

doi:10.1111/iej.12897

Cited by 43 publications

(35 citation statements)

References 62 publications

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“…Accordingly, the European Society of Endodontology (ESE) released a position statement indicating a clinical protocol for revitalization in 2016 (ESE ). Furthermore, residual bacteria and endotoxins have recently been related to a lack of root development (Verma et al ) and to prevent cells of entering odontoblast lineage (Vishwanat et al , Widbiller et al ). Thus, root canals need to be disinfected adequately to allow for more controlled stem cell fate in regenerative endodontic procedures (Diogenes & Hargraves ).…”

Section: Discussionmentioning

confidence: 99%

Biomechanical performance of an immature maxillary central incisor after revitalization: a finite element analysis

et al. 2019

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Aim To investigate the stress distribution in an immature maxillary incisor and the same tooth after simulated revitalization with deposition of tubular dentine or cementum by finite element analysis (FEA). Methodology A finite element model of a maxillary central incisor was developed on the basis of a µCT scan. The tooth was segmented in two parts: a part that represented a tooth in an immature state and an apical part that represented the tissue formed after revitalization. The apical part was given the mechanical properties of dentine or cementum. The immature tooth and the same tooth reinforced by either dentine or cementum underwent simulation of biting, trauma and orthodontic movement. Von Mises stress values were compared between the scenarios and tooth segments. Results Maximum stress in the immature incisor developed apically; however, dentine‐ and cementum‐reinforced teeth revealed the greatest stress in the external portion of the root decreasing towards the apex. Greatest mechanical stress was caused by dental trauma perpendicular to the long axis of the root followed by biting and orthodontic movement. Stress peaks were lower in the dentine‐reinforced tooth compared with the cementum‐reinforced tooth in all scenarios; however, median stress in the immature part was reduced irrespective of dentine or cementum deposition. Dentine reinforcement caused greater stress values in the apical segment due to absorbance of the applied force, whereas stress was not transferred towards deposited cementum. Conclusions Apposition of simulated hard tissue in a maxillary central incisor after revitalization reduced mechanical stress in the immature tooth. Formation of dentine was advantageous because, unlike cementum, it facilitated an even stress distribution throughout the root resulting in lower stress values.

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Section: Discussionmentioning

confidence: 99%

Biomechanical performance of an immature maxillary central incisor after revitalization: a finite element analysis

et al. 2019

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“…of mineralization 30,40,41 . Furthermore, dentin matrix proteins modulate the expression of chemokines 42 and induce angiogenesis and neurogenesis [43][44][45] .…”

Section: Figure 3 -mentioning

confidence: 99%

Cell-Free Approaches for Dental Pulp Tissue Engineering

Galler

Widbiller

2020

Journal of Endodontics

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In past years, both cell transplantation and cell homing have been explored for dental pulp tissue engineering. Sufficient evidence shows that after cell transplantation, the regeneration of a functional dentin-pulp complex is possible. A new milestone was reached recently. The concept has now been evaluated in clinical studies. However, the approach is afflicted with high efforts and operating expenses; thus cell homing might be a viable alternative. In this article, the latest developments on the recruitment of resident stem cells by dentin-derived growth factors in injectable fibrin-based scaffold materials will be discussed.

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“…Furthermore, selective caries excavation can be biologically advantageous because pulp exposure is avoided. Indirect pulp capping using bioactive materials may reduce the concentrations of bacterial lipopolysaccharides and lipoteichoic acid and it can provide a barrier against the potentially cytotoxic effects of adhesive system monomers (14‐16). Apart from the biological advantages of selective excavation, little is known about the influence of residual carious dentin on the mechanical properties of restorations (4,17).…”

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confidence: 99%

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

Scholz¹,

Hinderberger²,

Widbiller³

et al. 2020

European J Oral Sciences

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Selective caries excavation may support pulp preservation. This in vitro study investigated the influence of selective removal of demineralized dentin on marginal integrity of composite restorations as determined by dye penetration. Dentinal caries‐like lesions were produced in the approximal surfaces of 40 extracted human molars (ethylenediaminetetraacetate, 0.5 M, 96 h). The following test procedures were established: complete excavation, selective excavation, and caries‐free control. Two class II cavities with enamel at the cervical margins were prepared per tooth and demineralization volume was determined by micro‐computed tomography for the purpose of a stratified distribution to receive complete excavation or selective excavation. After complete or selective excavation (30 cavities each), adhesive composite restorations were placed. Cavities without demineralized dentin (20 cavities) served as control. The marginal integrity of restorations was evaluated by dye penetration with and without thermocycling or mechanical loading. Results were analyzed by non‐parametrical statistical tests (Mann‐Whitney U Test) with an α = 0.05 level of significance. Dye penetration did not differ significantly among completely excavated, selectively excavated, or undemineralized teeth, but was increased by thermocycling and mechanical loading in all experimental groups. Selective caries removal did not increase marginal penetration in class II restorations. The presence of remaining demineralized dentin surrounded by sound dentin did not impair marginal integrity of restorations with margins placed in sound enamel.

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Interactive effects of LPS and dentine matrix proteins on human dental pulp stem cells

Abstract: Lipopolysaccharide did not affect cell viability but interfered with odontoblast-like cell differentiation of DPSCs. Proteins from the dentine matrix may have a protective effect, attenuate the detrimental impact of LPS and thus play an important role during pulp repair.

Cited by 43 publications

References 62 publications

Biomechanical performance of an immature maxillary central incisor after revitalization: a finite element analysis

Biomechanical performance of an immature maxillary central incisor after revitalization: a finite element analysis

Cell-Free Approaches for Dental Pulp Tissue Engineering

Influence of selective caries excavation on marginal penetration of class II composite restorations in vitro

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