Interactive effects of UV, benzo[α] pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Paleomonetes pugio

Hook, Sharon E.; Lee, Richard F.

doi:10.1016/j.marenvres.2004.03.087

Cited by 30 publications

(16 citation statements)

References 7 publications

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“…An efficient DNA repair mechanism has been reported to be present in several aquatic invertebrates that include mussels (Harvey and Parry 1997). Single cell gel electrophoresis has been successfully employed in studying DNA repair mechanisms (Collins 2004;Hook and Lee 2004). Rejoining of DNA strand breaks by most cell types is known to be a rapid process (Frankenberg-Schwager 1989) and comet assay offers a great scope in the study of DNA repair kinetics (Collins 2004).…”

Section: Discussionmentioning

confidence: 99%

Application of comet assay in the study of DNA damage and recovery in rohu (Labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide

Mohanty

Nayak

et al. 2010

Ecotoxicology

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Labeo rohita (rohu) fingerlings were exposed to different concentrations (0.001, 0.002 and 0.01 ppm) of phorate, an organophosphate pesticide; samplings were done at 24, 48, 72 and 96 h. The study was carried out to evaluate tissue specific genotoxic effects produced by phorate, on three different tissue systems and to assess DNA repair response in fish. Results of tissue specific DNA damage experiments showed low baseline damage in blood cells followed by gill and liver cells in control individuals whereas more DNA breaks were found in liver followed by gill and blood cells of treated individuals. Concentrations-dependent DNA damage showed a strong, linear and positive relationship (r(2) = >0.7) in all three tissues. Clear time-related increase in DNA damage was observed for all tissues exposed to all concentrations except in liver cells at 0.01 ppm, where the DNA damage declined significantly after 72 h. For the assessment of DNA repair response, fingerlings were first exposed to 0.01 ppm of phorate for 72 h and then transferred to pesticide free water. Tissue chosen for the repair experiment was liver. Samplings were done at 0, 3, 6, 12 and 24 h after the release of 72 h pesticide treated fishes into pesticide free water. Fishes showed a reduction in DNA breaks from 3 h onwards in pesticide free water and at 24 h returned to control level damage. The results indicate that phorate is a potential genotoxicant, comet assay can be used in DNA damage and repair analysis, response to pollutants in multicellular animals is often tissue specific.

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Section: Discussionmentioning

confidence: 99%

Application of comet assay in the study of DNA damage and recovery in rohu (Labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide

Mohanty

Nayak

et al. 2010

Ecotoxicology

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“…In grass shrimp embryos Comet assay was used to provide a sensitive measure of DNA strand breakage exposed to genotoxicants, highway run off sediments, sediments with coal ash and phototoxicants (Lee et al, 2000(Lee et al, , 2008Lee and Kim, 2002;Kim and Lee, 2004). In the concentrations tested cadmium chloride and mercuric chloride induced Comets in 72% and 61% of the cells respectively after an exposure over a period of 4 h. Genotoxicity of cadmium was reported in rat hemispheres and cerebellum (Klimova and Misurova, 2002) in rat Leydig cells (Yang et al, 2003), in P. pugio embryos (Hook and Lee, 2004), in M. edulis (Pruski and Dixon, 2002;Emmanouil et al, 2007) and in L. vannamei (Chang et al, 2009). The mechanism of metal induced carcinogenesis is still unknown, but one possible pathway may involve the interaction of metals with DNA, either directly or indirectly (Hartmann and Speit, 1994).…”

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confidence: 93%

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Jose

Puthumana

Mohandas

et al. 2011

Marine Environmental Research

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a b s t r a c tLack of shrimp cell lines has hindered the study of pollutants which adversely affects shrimp health and its export value. In this context a primary haemocyte culture developed from Penaeus monodon was employed for assessing the cytotoxicity and genotoxicity of two heavy metal compounds, cadmium chloride and mercuric chloride and two organophosphate insecticides, malathion and monocrotophos.Using MTT assay 12 h IC 50 values calculated were 31.09 AE 16.27 mM and 5.52 AE 1.16 mM for cadmium chloride and mercuric chloride and 59.94 AE 52.30 mg l À1 and 186.76 AE 77.00 mg l À1 for malathion and monocrotophos respectively. Employing Comet assay, DNA damage inflicted by these pollutants on haemocytes were evaluated and the pollutants induced DNA damage in >60% of the cells. The study suggested that haemocyte culture could be used as a tool for quantifying cytotoxicity and genotoxicity of aquaculture drugs, management chemicals and pollutants.

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“…If the BER pathway is compromised a nucleotide excision repair (NER) pathway exists for the removal of the 8-oxo-dG adduct resulting in an observed reduction the amount of free 8-oxo-dG in the hemolymph. A study undertaken by Hook and Lee (2004) found that exposure of P. pugio embryos to B[a]P produced complex lesions. The DNA strand breaks persisted and their level increased as these lesions were repaired by the NER pathway, whereas repairs by the BER pathway resulted in reduction of strand breaks when the embryos were exposed to cadmium.…”

Section: Discussionmentioning

confidence: 99%

Freshwater shrimp (Palaemonetes australis) as a potential bioindicator of crustacean health

Webb

2010

Environ Monit Assess

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Interactive effects of UV, benzo[α] pyrene, and cadmium on DNA damage and repair in embryos of the grass shrimp Paleomonetes pugio

Cited by 30 publications

References 7 publications

Application of comet assay in the study of DNA damage and recovery in rohu (Labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide

Application of comet assay in the study of DNA damage and recovery in rohu (Labeo rohita) fingerlings after an exposure to phorate, an organophosphate pesticide

Application of primary haemocyte culture of Penaeus monodon in the assessment of cytotoxicity and genotoxicity of heavy metals and pesticides

Freshwater shrimp (Palaemonetes australis) as a potential bioindicator of crustacean health

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