Interactive involvement of hippocampal cAMP/PKA and cyclooxygenase-2 signaling pathways in spatial learning in the Morris water maze

Abstract

Introduction: Accumulated evidence shows that the cAMP-PKA signaling pathway plays a key role in memory functions. Cyclooxygenase-2, a critical player in neuroinflammation, has been confirmed in the pathogenesis of neurodegenerative diseases. This study is aimed to assess the effect of the interaction of cAMP-PKA and cyclooxygenase pathways on spatial memory acquisition in animal models. Material and methods: In the present study, the effects of the four-day bilateral intra-hippocampal infusions of H-89 as a protein kinase AII inhibitor (10 μM/side), celecoxib (0.1 M/side) as a selective cyclooxygenase-2 inhibitor, celecoxib/H-89 and bucladesine (10 μM/side)/celecoxib/H-89 on spatial memory acquisition in the Morris water maze were investigated. Control animals received bilateral intra-hippocampal infusions of dimethyl sulfoxide. Rats were trained for 4 days; each day included one block of four trials. Post-training probe trial tests were performed on day five. Results: A bilateral intra-hippocampal infusion of H-89 and celecoxib led to a significant impairment in spatial learning compared to the controls through a notable decrease in escape latency and traveled distance. But, combination treatment of animals with celecoxib/H-89 and bucladesine/celecoxib/H-89 could considerably reverse celecoxib and H-89-induced spatial memory acquisition impairments in the Morris water maze. Conclusions: These results indicate the probable regulatory effects of cAMP/PKA and cyclooxygenase-2 signaling pathways on spatial memory acquisition in the Morris water maze.

Tadalafil Reversed H-89 – and Scopolamine – Induced Spatial Learning Impairments in Male Rats

“…Inhibition of PKA signaling pathway causes learning and memory deficiency [11,12] and increase in apoptosis and autophagy [13]. Our previous studies in rats have also illustrated that intra-hippocampal infusion of H-89 impairs spatial memory formation in Morris water maze (MWM) task [14,15]. It has also been shown that post-training bilateral intrahippocampal (i.h.)…”

Restorative effect of modified dioscorea pills on the structure of hippocampal neurovascular unit in an animal model of chronic cerebral hypoperfusion

“…The equipment was supplied by Chengdu Taimeng Science and Technology Co., Ltd., China. The test was began on the 40th day after the first day of gavaging, including navigation test and probing test as other studies [11]. The day after MWM test finishing, rats were capitalized and brain tissues were obtained through perfusion sampling and fresh sampling.…”

The seed of Litchi chinensis fraction ameliorates hippocampal neuronal injury in an Aβ25-35-induced Alzheimer’s disease rat model via the AKT/GSK-3β pathway

“…The Morris water maze is the most frequently used device in behavioural neuroscience. The standard detection procedure has been described previously (Deng et al 2016;Tabrizian et al 2018). Briefly, the experiment was carried out in a round white pool (size, 94 cm in diameter and 31 cm deep), filled with water to a depth of 30 cm at a temperature of $25 C. A 25-cm 2 Plexiglas square was used as the escape platform and was placed in the centre of one quadrant in the pool, $15 cm from the pool's edge, and submerged 1 cm beneath the water surface.…”