Objectives: We studied the association between objectively-measured smartphone usage and objectively-measured sleep quality and physical activity for seven consecutive days among Hong Kong adolescents and young adults aged 11–25 (n = 357, 67% female).Methods: We installed an app that tracked the subjects’ smartphone usage and had them wear an ActiGraph GT3X accelerometer on their wrist to measure their sleep quality and physical activity level. Smartphone usage data were successfully obtained from 187 participants (52.4%).Results: The participants on average spent 2 hours 46 minutes per day on their smartphone. Multilevel regression showed that, among secondary school students, one minute of daytime smartphone usage was associated with 0.12 minute decrease in total sleeping time that night (p = 0.042, 95% CI: -0.23, -0.007). One minute of bedtime smartphone usage was associated with 0.32 minute increase in wake after sleep onset that night (p = 0.04, 95% CI: 0.02, 0.62). One minute of smartphone usage during sleep was associated with sleep efficiency (β = 0.013%, p = 0.01, 95% CI: 0.003%, 0.023%) and WASO (β=-0.05, p = 0.04, 95% CI: -0.10, -0.005). One minute of daytime smartphone usage was associated with 7.15 steps increase in the number of steps (p = 0.02, 95% CI: 1.02, 13.28) among secondary school students and 3.52 steps increase in the number of steps (p = 0.03, 95% CI: 0.37, 6.66) among university students on the next day.Conclusion: Time spent on smartphone was associated with total sleeping time, the number of steps, and MVPA among Hong Kong adolescents and young adults.