Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication

Kuo, Rei-Lin; Li, Zong-Hua; Li, Li-Hsin; Lee, Kuo-Ming; Tam, Ee-Hong; Liu, Helene M.; Liu, Hao‐Ping; Shih, Shin‐Ru; Wu, Chih‐Ching

doi:10.1021/acs.jproteome.6b00103

Cited by 33 publications

(44 citation statements)

References 50 publications

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“…Previous studies from our laboratory have shown that the 14-3-3 protein also binds to the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid (N) protein [79]. This binding allows the regulation of nucleocytoplasmic shuttling of N protein in a phosphorylation-dependent manner.…”

Section: Role Of 14-3-3 Family In Rna Virusesmentioning

confidence: 99%

“…The apoptosis pathway, cell cycle, cell signaling, ubiquitin ligase, and nucleocytoplasmic shuttling are the most common pathways which were usurped and manipulated by the viruses to favor their replication. [78,79] We also elaborate on all the signaling pathways that are manipulated by 14-3-3-virus interactions (Figure 3). It is interesting to note that cellular proteins are essential for the replication of many RNA and DNA viruses, and may serve as viable targets for treating viral infections.…”

Section: Role Of 14-3-3 In Dna Virusesmentioning

confidence: 99%

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The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

Nathan

Lal

2020

Viruses

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The 14-3-3 proteins are a family of ubiquitous and exclusively eukaryotic proteins with an astoundingly significant number of binding partners. Their binding alters the activity, stability, localization, and phosphorylation state of a target protein. The association of 14-3-3 proteins with the regulation of a wide range of general and specific signaling pathways suggests their crucial role in health and disease. Recent studies have linked 14-3-3 to several RNA and DNA viruses that may contribute to the pathogenesis and progression of infections. Therefore, comprehensive knowledge of host–virus interactions is vital for understanding the viral life cycle and developing effective therapeutic strategies. Moreover, pharmaceutical research is already moving towards targeting host proteins in the control of virus pathogenesis. As such, targeting the right host protein to interrupt host–virus interactions could be an effective therapeutic strategy. In this review, we generated a 14-3-3 protein interactions roadmap in viruses, using the freely available Virusmentha network, an online virus–virus or virus–host interaction tool. Furthermore, we summarize the role of the 14-3-3 family in RNA and DNA viruses. The participation of 14-3-3 in viral infections underlines its significance as a key regulator for the expression of host and viral proteins.

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Section: Role Of 14-3-3 Family In Rna Virusesmentioning

confidence: 99%

Section: Role Of 14-3-3 In Dna Virusesmentioning

confidence: 99%

The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

Nathan

Lal

2020

Viruses

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“…In addition to serving as an IFN antagonist, accumulating evidence indicates a modulatory role of NS1 on cellular and viral gene expression (Falcon et al, 2004;Wang et al, 2010;Kuo et al, 2016b). We exploited a mini-genome reporter assay to determine the possible role of NS1 on viral RdRp activity.…”

Section: Effect Of Non-structural Protein 1 On Viral Genome Activationmentioning

confidence: 99%

The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses

et al. 2020

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Avian influenza virus (AIV) can cause severe diseases in poultry worldwide. H6N1 AIV was the dominant enzootic subtype in 1985 in the chicken farms of Taiwan until the initial outbreak of a low pathogenic avian influenza (LPAI) H5N2 virus in 2003; thereafter, this and other LPAIs have been sporadically detected. In 2015, the outbreak of three novel H5Nx viruses of highly pathogenic avian influenza (HPAI) emerged and devastated Taiwanese chicken and waterfowl industries. The mechanism of variation in pathogenicity among these viruses is unclear; but, in light of the many biological functions of viral non-structural protein 1 (NS1), including interferon (IFN) antagonist and host range determinant, we hypothesized that NS genetic diversity contributes to AIV pathogenesis. To determine the impact of NS1 variants on viral infection dynamics, we established a reverse genetics system with the genetic backbone of the enzootic Taiwanese H6N1 for generation of reassortant AIVs carrying exogenous NS segments of three different Taiwanese H5N2 strains. We observed distinct cellular distributions of NS1 among the reassortant viruses. Moreover, exchange of the NS segment significantly influenced growth kinetics and induction of cytokines [IFN-α, IFN-β, and tumor necrosis factor alpha (TNF-α)] in an NS1-and host-specific manner. The impact of NS1 variants on viral replication appears related to their synergic effects on viral RNA-dependent RNA polymerase activity and IFN response. With these approaches, we revealed that NS1 is a key factor responsible for the diverse characteristics of AIVs in Taiwan.

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“…The m/z scan range for the MS scans was set as 350-2000 Da, and the ion signal of (Si (CH 3 ) 2 O) 6 H + at m/z 445.120025 was used as a lock mass for internal calibration. To increase identification coverage, the precursor ions selected for MS/MS analysis were dynamically excluded for 180 s [46][47][48] .…”

Section: In-gel Digestion and Mass Spectrometric Analysismentioning

confidence: 99%

Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization

Wang

Huang

et al. 2020

Oncogenesis

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The aberrant subcellular translocation and distribution of epidermal growth factor receptor (EGFR) represent a major yet currently underappreciated cancer development mechanism in non-small cell lung cancer (NSCLC). In this study, we investigated the subcellular interactome of EGFR by using a spectral counting-based approach combined with liquid chromatography-tandem mass spectrometry to understand the associated protein networks involved in the tumorigenesis of NSCLC. A total of 54, 77, and 63 EGFR-interacting proteins were identified specifically in the cytosolic, mitochondrial, and nuclear fractions from a NSCLC cell line, respectively. Pathway analyses of these proteins using the KEGG database shown that the EGFR-interacting proteins of the cytosol and nucleus are involved in the ribosome and spliceosome pathways, respectively, while those of the mitochondria are involved in metabolizing propanoate, fatty acid, valine, leucine, and isoleucine. A selected nuclear EGFR-interacting protein, hnRNP A3, was found to modulate the accumulation of nuclear EGFR. Downregulation of hnRNP A3 reduced the nuclear accumulation of EGFR, and this was accompanied by reduced tumor growth ability in vitro and in vivo. These results indicate that variations in the subcellular translocation and distribution of EGFR within NSCLC cells could affect tumor progression.

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Interactome Analysis of the NS1 Protein Encoded by Influenza A H1N1 Virus Reveals a Positive Regulatory Role of Host Protein PRP19 in Viral Replication

Cited by 33 publications

References 50 publications

The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

The Multifarious Role of 14-3-3 Family of Proteins in Viral Replication

The Impacts of Reassortant Avian Influenza H5N2 Virus NS1 Proteins on Viral Compatibility and Regulation of Immune Responses

Profiling of subcellular EGFR interactome reveals hnRNP A3 modulates nuclear EGFR localization

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