Interbacterial aggregation of plaque bacteria

Gibbons, R. J.; Nygaard, Mai

doi:10.1016/0003-9969(70)90031-2

Cited by 326 publications

(238 citation statements)

References 2 publications

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“…naeslundii was often observed in mixed clusters with streptococci and other bacteria at 6 and 12 h. This observation supports the view that co-adhesion, in particular co-adhesion processes involving A. naeslundii, streptococci and other bacteria, play an important role during the initial stages of colonization of tooth surfaces (Bos et al, 1996;Gibbons & Nygaard, 1970;Kolenbrander, 1988;Kolenbrander et al, 1990;Palmer et al, 2003;Yoshida et al, 2006). This observation is further supported by the finding of genotypically different bacteria colocalizing at the outer surface of the biofilm, indicating that co-adhesion of bacteria from saliva is a continuing process adding to the biomass of the developing biofilm.…”

Section: Discussionsupporting

confidence: 79%

Actinomyces naeslundii in initial dental biofilm formation

et al. 2009

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The combined use of confocal laser scanning microscopy (CLSM) and fluorescent in situ hybridization (FISH) offers new opportunities for analysis of the spatial relationships and temporal changes of specific members of the microbiota of intact dental biofilms. The purpose of this study was to analyse the patterns of colonization and population dynamics of Actinomyces naeslundii compared to streptococci and other bacteria during the initial 48 h of biofilm formation in the oral cavity. Biofilms developed on standardized glass slabs mounted in intra-oral appliances worn by ten individuals for 6, 12, 24 and 48 h. The biofilms were subsequently labelled with probes against A. naeslundii (ACT476), streptococci (STR405) or all bacteria (EUB338), and were analysed by CLSM. Labelled bacteria were quantified by stereological tools. The results showed a notable increase in the number of streptococci and A. naeslundii over time, with a tendency towards a slower growth rate for A. naeslundii compared with streptococci. A. naeslundii was located mainly in the inner part of the multilayered biofilm, indicating that it is one of the species that attaches directly to the acquired pellicle. The participation of A. naeslundii in the initial stages of dental biofilm formation may have important ecological consequences. INTRODUCTIONDental biofilm is an archetypal example of a complex biofilm (Costerton et al., 1999;Davies, 2003;DuPont, 1997). Biofilm formation on tooth surfaces follows the same basic rules as biofilm formation elsewhere in nature. Dental biofilms develop readily because of the optimal temperature, the rich nutrient supply in the oral cavity, and the hard non-shedding surface. They are easily accessible for experimentation using intra-oral devices (Auschill et al., 2004;Kilian et al., 1979;Nyvad & Fejerskov, 1987a;Palmer et al., 2003), and therefore dental biofilms can be used to demonstrate colonization phenomena and ecological principles of universal interest. Concurrent with the increasing recognition of the significance of biofilms in infectious diseases, the development of techniques such as confocal laser scanning microscopy (CLSM), fluorescence in situ hybridization (FISH) and immunofluorescence has enabled visualization of bacteria in their natural undisturbed environment. This offers a substantial improvement upon previous microbiological studies of bacteria grown in planktonic settings (Anwar et al., 1992;Davies, 2003).Previous studies of dental biofilm that took advantage of these methods mainly focused on streptococci Dige et al., 2007; Hannig et al., 2007;Palmer et al., 2003) because culture-based studies suggested that this group of bacteria is prominent during the initial stages of biofilm formation on teeth (Li et al., 2004;Nyvad & Kilian, 1987. However, other genera such as Actinomyces are also among the earliest colonizers of dental surfaces and may constitute up to 27 % of the pioneer bacteria (Kilian et al., 1979;Li et al., 2004;Nyvad & Kilian, 1987). Several culturebased studies indicated that Ac...

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Section: Discussionsupporting

confidence: 79%

Actinomyces naeslundii in initial dental biofilm formation

et al. 2009

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“…Early in the 1970s, coaggregation was already demonstrated to be a common phenomenon between isolates from dental plaque [34][35]. Coaggregation has been detected between hundreds of the culturable oral bacteria, and has been proposed as fundamental process during dental plaque biofilm formation [36].…”

Section: Structure Development Of Multiple-species Biofilmsmentioning

confidence: 99%

Current understanding of multi‐species biofilms

et al. 2011

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Direct observation of a wide range of natural microorganisms has revealed the fact that the majority of microbes persist as surface-attached communities surrounded by matrix materials, called biofilms. Biofilms can be formed by a single bacterial strain. However, most natural biofilms are actually formed by multiple bacterial species. Conventional methods for bacterial cleaning, such as applications of antibiotics and/or disinfectants are often ineffective for biofilm populations due to their special physiology and physical matrix barrier. It has been estimated that billions of dollars are spent every year worldwide to deal with damage to equipment, contaminations of products, energy losses, and infections in human beings resulted from microbial biofilms. Microorganisms compete, cooperate, and communicate with each other in multi-species biofilms. Understanding the mechanisms of multi-species biofilm formation will facilitate the development of methods for combating bacterial biofilms in clinical, environmental, industrial, and agricultural areas. The most recent advances in the understanding of multi-species biofilms are summarized and discussed in the review.

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“…[13][14][15][16] Co-aggregation is induced by non-specific physicochemical or specific biological interactions. [17][18][19] Here, we investigated that the co-aggregative properties and mechanisms of various Lactobacilli cells with E. coli cells.…”

Section: )mentioning

confidence: 99%

Fimbriae and lipopolysaccharides are necessary for co-aggregation between Lactobacilli and Escherichia coli

Mizuno

Furukawa

Usui

et al. 2014

Bioscience, Biotechnology, and Biochemistry

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Cells of Lactobacilli co-aggregated with Escherichia coli K-12 cells to form co-aggregates under mixed-culture conditions at 37 °C for 24 h. Co-aggregation was inhibited by sodium dodecyl sulfate but not by protease. E. coli deletion mutants of fimbriae formation and lipopolysaccharide (LPS) formation did not co-aggregate with Lactobacilli. These results showed that fimbriae and LPS are necessary for co-aggregation between Lactobacilli and E. coli.

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Interbacterial aggregation of plaque bacteria

Cited by 326 publications

References 2 publications

Actinomyces naeslundii in initial dental biofilm formation

Actinomyces naeslundii in initial dental biofilm formation

Current understanding of multi‐species biofilms

Fimbriae and lipopolysaccharides are necessary for co-aggregation between Lactobacilli and Escherichia coli

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