Interbilayer interactions from high-resolution x-ray scattering

Petrache, Horia I.; Gouliaev, Nikolai; Tristram-Nagle, Stephanie; Zhang, Wei; Suter, Robert; Nagle, John F.

doi:10.1103/physreve.57.7014

Cited by 262 publications

(374 citation statements)

References 35 publications

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“…Here, k is the bending rigidity, also called bending elastic modulus. For phosphatidylcholine bilayers in the liquid phase k is typically 10 À19 J [782][783][784][785][786]. For the spontaneous adsorption of a bilayer, the adsorption energy must be higher than the bending energy.…”

Section: Force Curves On Lipid Bilayersmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,242

2,949

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Section: Force Curves On Lipid Bilayersmentioning

confidence: 99%

Force measurements with the atomic force microscope: Technique, interpretation and applications

Butt

Cappella

Kappl

2005

Surface Science Reports

3,242

2,949

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“…By plotting the electrostatic pressure Π [19] as a function of the interbilayer water thickness d w (Fig. 3.A), all points fall on a master curve obtained for both the natural entropic repulsion between bilayers [24] and osmotic stress, either applied on floating bilayers [24] or multilayer stacks [30], demonstrating that the local electromagnetic stress is well described by our model. We also report in Fig.…”

Section: Fig 1: Schematic Representation Of the Effect Of Electric Fmentioning

confidence: 99%

“…Empty symbols are from Ref. [30] (osmotic stress on multilayer stacks). The solid line is calculated after Ref.…”

Section: Fig 1: Schematic Representation Of the Effect Of Electric Fmentioning

confidence: 99%

Reduction in Tension and Stiffening of Lipid Membranes in an Electric Field Revealed by X-Ray Scattering

et al. 2016

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The effect of AC electric fields on the elasticity of supported lipid bilayers has been investigated at the microscopic level using grazing incidence synchrotron x-ray scattering. A strong decrease in the membrane tension up to 1mN/m and a dramatic increase of its effective rigidity up to 300k B T are observed for local electric potentials seen by the membrane 1V. The experimental results were analyzed using detailed electrokinetic modeling and non-linear Poisson-Boltzmann theory. Based on a modeling of the electromagnetic stress which provides an accurate description of bilayer separation vs pressure curves, we show that the decrease in tension results from the amplification of charge fluctuations on the membrane surface whereas the increase in bending rigidity results from direct interaction between charges in the electric double layer. These effects eventually lead to a destabilization of the bilayer and vesicle formation. Similar effects are expected at the tens of nanometer lengthscale in cell membranes with lower tension, and could explain a number of electrically driven processes.Electric fields can be used to destabilize lipid bilayers as in the electroformation process, the most popular method to form large unilamellar vesicles [1], or to manipulate the shape of vesicles [2][3][4]. Beyond biosensor applications and the investigation of fundamental mechanical, dynamical and binding properties of membranes using impedance spectroscopy or dielectric relaxation [5], the strong influence of electric fields on lipid membrane behavior is also used in numerous applications in cell biology, biotechnology and pharmacology [6, 7] such as cell hybridization [8], electroporation [9], electrofusion [10] and electropermeabilization [11]. All these effects imply a strong deformation of the membranes in the field, the understanding of which in terms of elastic properties is therefore of prime importance [12]. Theoretically, the effect of electric fields on membrane tension has been investigated in Ref. [13], which was extended to bending rigidity in Refs [14][15][16][17][18]. When placed in an electric field E, charges of opposite sign will accumulate at both sides of a membrane which can be seen as a capacitor with surface charge densities Σ ± (see Fig.1.A), allowing to calculate the normal component of the electromagnetic stress Σ 2 + − Σ 2 − /ǫ m [19]. For a flat membrane, a direct consequence is electrostriction: at equilibrium, the elastic response of the membrane (Young modulus ∼ 10 7 − 10 8 Pa [5,20]) bal- * Present address: Max Planck Institute for Dynamics and SelfOrganization (MPIDS), Göttingen 37077, Germany † thierry.charitat@ics-cnrs.unistra.fr ances the electrostatic pressure [21]. Beyond this simple effect, membrane fluctuations modify the boundary conditions for the electric field, leading to a subtle coupling between electrostatics and membrane elasticity. Due to membrane finite thickness d m , a bending deformation induces surface element variations of opposite sign on both interface leading to a net ...

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“…However, most conventional soft and wet hydrogels usually show extremely poor functions due to their amorphous structure, i.e., random cross-linking polymer chain at molecular level, in contrast with the natural bio-tissue that possesses well-defined hierarchy structure from molecular level to macroscopic scale. The well ordered anisotropic structures of the biological tissues enable highly elaborate functions of living organisms [11][12][13][14][15]. For example, actins and myosin show a liquid crystalline anisotropic structure in a muscle sarcomere, which contributes the smooth motion of muscle fibers and muscle contraction in a specific direction [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Multi-functions of hydrogel with bilayer-based lamellar structure

Haque

Gong

2013

Reactive and Functional Polymers

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A novel hybrid hydrogel has been developed by combining bilayer-based lamellar structure of a self-assembled polymer surfactant and polymer network of conventional hydrogel system. A wide range of lamellar structure from micro-domain up to macro-domain (cm-scale) has been successfully generated in the hydrogel. Flat, infinitely large, and perfectly aligned lamellar macro-domain was formed by applying mechanical shear to the gel forming precursor solution containing monomer, cross-linker, and initiator. The obtained hydrogel system contains macroscopic, single-domain, periodical stacking of integrated microscopic lamellar bilayers inside the polymer matrix of the hydrogel. Periodical stacking of the bilayers in the hydrogel selectively diffract visible light to exhibit magnificent structural color. Due to the uniaxial orientation of the bilayer, the hydrogel possesses superb functions that have never been realized before, such as the one-dimensional swelling, anisotropic Young's modulus, anisotropic molecular permeation, and diffusion. Furthermore, the hydrogel exhibits excellent color tuning ability over a wide spectrum range by mechanical stimuli.3

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Interbilayer interactions from high-resolution x-ray scattering

Cited by 262 publications

References 35 publications

Force measurements with the atomic force microscope: Technique, interpretation and applications

Force measurements with the atomic force microscope: Technique, interpretation and applications

Reduction in Tension and Stiffening of Lipid Membranes in an Electric Field Revealed by X-Ray Scattering

Multi-functions of hydrogel with bilayer-based lamellar structure

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