Intercalation of Organic Dye Molecules into Double‐stranded DNA. Part 2: The Annelated Quinolizinium Ion as a Structural Motif in DNA Intercalators<sup>†</sup>

Ihmels, Heiko; Faulhaber, Katja; Vedaldi, Daniela; Dall’Acqua, Francesco; Viola, Giampietro

doi:10.1562/2005-01-25-ir-427

Cited by 109 publications

(103 citation statements)

References 57 publications

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“…9 Its biological activity and interaction with polynucleotides have been recently reviewed. 10 Spectrophotometric measurements demonstrated that it forms dimers and multiple aggregates in water. The increase of the ionic strength or the presence of small amount of DNA was found to promote the association.…”

Section: Introductionmentioning

confidence: 99%

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Megyesi

Biczók

Görner

2009

Photochem Photobiol Sci

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The effects of anionic chondroitin or dextran sulfates on the absorption and fluorescence properties of coralyne, a cationic benzo[c]phenanthridine type alkaloid, were studied in aqueous solution. The remarkably strong binding to both polysaccharides promotes the coralyne dimer formation, which was evidenced by the changes in the absorption and fluorescence spectra and the fluorescence decay. The extent of dimerization, induced by chondroitin, shows a significant pH dependence because the competitive protonation of the carboxylate moieties of the polymer chain decreases the number of binding sites. A larger molecular weight of dextran sulfate stabilizes the coralyne dimer more efficiently.

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Section: Introductionmentioning

confidence: 99%

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Megyesi

Biczók

Görner

2009

Photochem Photobiol Sci

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“…Some studies suggest that berberine may either directly bind duplex DNA by intercalation (27)(28)(29)(30) or inflict DNA damage indirectly by its interaction with topoisomerases I and II (28,(31)(32)(33). Other investigators also showed that berberine at 150 mg/ml was able to induce the rate of sister chromatid exchanges, which reflects homologous recombination repair, by several fold (34).…”

Section: Discussionmentioning

confidence: 99%

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

et al. 2009

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Abstract. The cytotoxicity of berberine on C6 rat glioma cells indicated that berberine induced morphological changes and caused cell death through G2/M arrest and apoptosis. While undergoing apoptosis, there was a remarkable accumulation of G2/M cells with the upregulatoin of Wee1 but it also inhibited cyclin B, CDK1 and Cdc25c that led to G2/M arrest. Along with cytotoxicity in C6 cells, several apoptotic events including mitochondrial cytochrome c release, activation of caspase-9, -3 and -8 and DNA fragmentation were induced. Berberine increased the levels of GADD153 and GRP 78 in C6 cells based on the examination of Western blotting and this is a major hallmark of endoplasmic reticulum (ER) stress. We also found that berberine promoted the production of reactive oxygen species and Ca 2+ in C6 cells. Western blotting assay also showed that berberine inhibited the levels of anti-apoptotic protein Bcl-2 but increased the levels of pro-apoptotic protein Bax before leading to a decrease in the levels of mitochondrial membrane potential (Δae m ) followed by cytochrome c release that caused the activations of capase-9 and -3 for apoptotic occurrence. The caspase-8, -9 and -3 were activated by berberine in C6 cells based on the substrate solution (PhiPhiLux-G 1 D 1 , CaspaLux 8-L 1 D 2 , CaspaLux 9-M 1 D 2 for caspase-3, -8 and -9, respectively) and analyzed by flow cytometer and each inhibitor of caspase-8, -9 and -3 led to increase the percentage of viable C6 cells after exposure to berberine. This finding was also confirmed by Western blot assay which showed that berberine promoted the active form of caspase-8, -9 and -3. These results demonstrate that the cytotoxicity of berberine in C6 rat glioma cells is attributable to apoptosis mainly through induced G2/M-arrested cells, in an ER-dependent manner, via a mitochondria-dependent caspase pathway regulated by Bax and Bcl-2.

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“…heterodimers among these proteins (29)(30)(31). The balance between the expression levels of the protein units (e.g.…”

Section: Discussionmentioning

confidence: 99%

Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells

Lai

Hsu

et al. 2009

Oncol Rep

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Abstract. Curcumin, a naturally occurring yellow pigment isolated from turmeric, is a well known antioxidant with broad spectrum of anti-tumor activities against many human cancer cells. In this study, curcumin-induced cytotoxic effect of mouse-rat hybrid retina ganglion cells (N18) were investigated. For determining cell viability, the trypan blue exclusion and flow cytometric analysis were used. The curcumin-caused cell cycle arrest in N18 cells was examined by flow cytometry. Curcumin affect on the production of reactive oxygen species and Ca 2+ and on the decrease of the level of mitochondria membrane potential (Δae m ) were also examined by flow cytometry. Curcumin-induced apoptosis was determined by DAPI staining and Western blotting was used for examining the apoptotic signaling proteins. Cell cycle analysis showed that G2/M phase arrest and sub-G1 occurs in N18 cells following 48 h exposure to curcumin. Curcumin also caused a marked increase in apoptosis, as characterized by DNA fragmentation (sub-G1 phase formation) and DAPI staining, and dysfunction of mitochondria, which was associated with the activation of caspase-8, -9 and -3. Curcumin also promoted the levels of Fas and FADD, Bax, cytochrome c release, but decreased the levels of Bcl-2 causing changes of Δae m . Curcumin also induced endoplasmic reticulum stress in N18 cells which was based on the changes of GADD153 and GRP78 and caused Ca 2+ release. Curcumin induced apoptosis through the intrinsic pathway and caspase-3-dependent and -independent pathways in N18 cells.

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Intercalation of Organic Dye Molecules into Double‐stranded DNA. Part 2: The Annelated Quinolizinium Ion as a Structural Motif in DNA Intercalators^†

Cited by 109 publications

References 57 publications

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells

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Intercalation of Organic Dye Molecules into Double‐stranded DNA. Part 2: The Annelated Quinolizinium Ion as a Structural Motif in DNA Intercalators†

Cited by 109 publications

References 57 publications

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Dimer-promoted fluorescence quenching of coralyne by binding to anionic polysaccharides

Involvement of reactive oxygen species and caspase-dependent pathway in berberine-induced cell cycle arrest and apoptosis in C6 rat glioma cells

Curcumin induces apoptosis through FAS and FADD, in caspase-3-dependent and -independent pathways in the N18 mouse-rat hybrid retina ganglion cells

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Intercalation of Organic Dye Molecules into Double‐stranded DNA. Part 2: The Annelated Quinolizinium Ion as a Structural Motif in DNA Intercalators^†