2015
DOI: 10.1016/j.ecolind.2015.04.021
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Intercalibration of ecotoxicity testing protocols with Artemia franciscana

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Cited by 39 publications
(26 citation statements)
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References 17 publications
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“…Besides, our data are similar to 96 h LC 50 values reported for DEG exposed Artemia in Tornambè et al (2012). The results on SDS are within the 14d LC 50 range obtained for SDS during an Artemia intercalibration exercise based on the same method; the exercise was organized in Italy at national level (Manfra et al 2015b). …”
Section: Resultssupporting
confidence: 74%
“…Besides, our data are similar to 96 h LC 50 values reported for DEG exposed Artemia in Tornambè et al (2012). The results on SDS are within the 14d LC 50 range obtained for SDS during an Artemia intercalibration exercise based on the same method; the exercise was organized in Italy at national level (Manfra et al 2015b). …”
Section: Resultssupporting
confidence: 74%
“…The most recent paper by Rotini et al (2015) compared the results of hatching, acute mortality and chronic mortality tests on DEG and SDS ranking the endpoints as follows: acute mortality < hatchability < chronic mortality. The protocol described in Rotini et al (2015) was intercalibrated at Italian level between 2006-2009 involving eleven laboratories to ring toxicity tests with A. franciscana using CuSO 4 as reference toxicant (Manfra et al, 2015b).…”
Section: Acute Cyst Hatching Testmentioning
confidence: 99%
“…Between 2006-2009, the 24 h mortality test with A. franciscana was intercalibrated together hatching assay (Manfra et al, 2015b). Three inter-comparison results constituted the dataset for calculating the 24 h LC 50 mean and the repeatability/reproducibility coefficients on CuSO 4 .…”
Section: Acute Mortality Testmentioning
confidence: 99%
“…is widely used as a test organism in a large number of ecotoxicology studies [10,7,14]. Short-term toxicity tests are the most common among studies and, usually, they include various endpoints such as survival, hatching, and behavioral patterns [24]. The majority of cyst-based toxicity assays on Artemia refer to a well-accepted endpoint as a criterion, which is known as the mortality of recently hatched nauplii (instar II-III stage) [7].…”
Section: Discussionmentioning
confidence: 99%
“…Before the actual toxicity tests, a "preliminary" test was performed (data not shown), in order to determine the "critical range" for each toxicant and exposure time [10]. For estimating LC 50 of SDS, the solutions used were 7, 8, 9, 10, and 12 ppm for all three exposure periods (24,48 Acute mortality tests were performed in multi-well plates (4 × 6 wells, Greiner CELLSTAR ® ). In each well/replicate, 1 mL of each solution was placed and 10 Artemia nauplii (Instar I-II) were transferred with a micropipette, whereas control wells contained only SSW and nauplii.…”
Section: Acute Mortality Testsmentioning
confidence: 99%