Intercapillary bridging cells: Immunocytochemical characteristics of cells that connect blood vessels in the retina

Mendes-Jorge, Luísa; Llombart, Cristina; Ramos, David; López-Luppo, Mariana; Valença, Andreia; Nacher, Víctor; Navarro, Marc; Carretero, Ana; Méndez-Ferrer, Simón; Rodríguez‐Baeza, Alfonso; Ruberte, Jesús

doi:10.1016/j.exer.2012.03.010

Cited by 27 publications

(22 citation statements)

References 43 publications

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“…A number of advanced imaging technologies have been used to study the rodent retinal vasculature, including confocal scanning laser microscopy (Mendes-Jorge et al, 2012;Paques et al, 2006;Ramos et al, 2013;Ritter et al, 2005) as well as scanning laser ophthalmoscopy and optical coherence tomography (SLO-OCT) (Ali Rahman et al, 2011;McLenachan et al, 2013;Paques et al, 2007Paques et al, , 2006Rakoczy et al, 2010;Zhi et al, 2014). Since SLO-OCT is commonly used in screening, diagnosis and staging of retinopathy in human, it is important that this technique is also used for the characterisation of mouse models of retinal vascular disease.…”

Section: Introductionmentioning

confidence: 99%

Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice

McLenachan¹,

Magno

Ramos

et al. 2015

Experimental Eye Research

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Section: Introductionmentioning

confidence: 99%

Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice

McLenachan¹,

Magno

Ramos

et al. 2015

Experimental Eye Research

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“…We received human eye samples without knowledge of the donor's identity, only biological data, such as sex and age, were available. Results obtained from the same human retinas were previously published [27], [28].…”

Section: Methodsmentioning

confidence: 86%

L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy

et al. 2014

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Iron is essential in the retina because the heme-containing enzyme guanylate cyclase modulates phototransduction in rods and cones. Transferrin endocytosis is the classical pathway for obtaining iron from the blood circulation in the retina. However, the iron storage protein ferritin has been also recently proposed as an iron carrier. In this study, the presence of Scara5 and its binding to L-ferritin was investigated in the retina. Our results showed that Scara5, the specific receptor for L-ferritin, was expressed in mouse and human retinas in many cell types, including endothelial cells. Furthermore, we showed that intravenously injected ferritin crossed the blood retinal barrier through L-ferritin binding to Scara5 in endothelial cells. Thus, suggesting the existence of a new pathway for iron delivery and trafficking in the retina. In a murine model of photoreceptor degeneration, Scara5 was downregulated, pointing out this receptor as a potential player implicated in retinopathy and also as a possible therapeutic target.

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“…These studies have framed the existence of pericyte-like bridges as evidence of pericyte detachment, where it is assumed that a fully-attached pericyte migrates (or begins to migrate) away from the capillary on which it resides and extends cell processes or its entire cell soma to form a bridge from one capillary to another [10][11][12][13] . Previous research has indicated that these bridging cells can colocalize with basement membrane structures that also span across, or bridge, adjacent capillaries 9,14 . These stand-alone (i.e.…”

Section: Introductionmentioning

confidence: 97%

“…Pericytes are considered an effector cell for microvascular remodeling and enwrap capillaries, maintaining close physical contact via cell soma and extended cellular processes within the vascular basement membrane 1 . However, some studies have observed pericyte-like cells bridging across two or more adjacent capillaries in both homeostatic 9 and pathological conditions such as diabetes, where their frequency is elevated 10 . These studies have framed the existence of pericyte-like bridges as evidence of pericyte detachment, where it is assumed that a fully-attached pericyte migrates (or begins to migrate) away from the capillary on which it resides and extends cell processes or its entire cell soma to form a bridge from one capillary to another [10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

“…These stand-alone (i.e. cell-free) basement membrane structures have been referred to in the literature as acellular capillaries 15 , intervascular bridges 9 , basal lamina and collagen-IV (Col-IV) sleeves 16 , and string vessels 15 . They also appear more frequently in pathological settings than in homeostasis, and some have presumed these basement membrane bridges to be residual structures left by collapsed and regressed capillaries (for review, please see 15 ).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pericyte Bridges in Homeostasis and Hyperglycemia: Reconsidering Pericyte Dropout and Microvascular Structures

Corliss

Ray

Doty

et al. 2019

Preprint

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Diabetic retinopathy threatens the vision of a third of diabetic patients. Progression of the disease is attributed to the dropout of pericytes, a cell type that enwraps and stabilizes the microvasculature. In tandem with this presumptive pericyte dropout, there is enriched formation of structures assumed to be remnants of collapsed or regressed vessels, previously classified as acellular capillaries, string vessels, and basement membrane bridges. Instead of endothelial cells, we show that pericytes colocalize with basement membrane bridges, and both bridging structures are enriched by cell-specific knockout of KLF4 and reversibly enriched with elevation of Ang-2, PDGF-BB, and blood sugar. Our data suggests that pericyte counts from retinal digests have misclassified pericyte bridges as endothelial structures and have exaggerated the role of pericyte loss in DR progression. In vivo imaging of corneal limbal vessels demonstrates pericyte migration off-vessel, implicating pericyte movement in formation of pericyte bridges and pathogenesis of diabetic retinopathy.

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Intercapillary bridging cells: Immunocytochemical characteristics of cells that connect blood vessels in the retina

Cited by 27 publications

References 43 publications

Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice

Angiography reveals novel features of the retinal vasculature in healthy and diabetic mice

L-Ferritin Binding to Scara5: A New Iron Traffic Pathway Potentially Implicated in Retinopathy

Pericyte Bridges in Homeostasis and Hyperglycemia: Reconsidering Pericyte Dropout and Microvascular Structures

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