Intercellular adhesion molecule 1-dependent activation of interleukin 8 expression in Candida albicans-infected human gingival epithelial cells

Abstract

Increased induction of interleukin 8 (IL-8) and intercellular adhesion molecule 1 (ICAM-1) by oral epithelial cells may play a role in the host defense mechanism in oropharyngeal candidiasis; however, little is known about the expression feature of these molecules on human gingival epithelial cells (HGECs) during Candida albicans infection. In this report we present evidence that neutralization with antibody against ICAM-1 inhibited both the adherence of C. albicans to HGECs and the Candida-induced production of IL-8, suggesting a role for ICAM-1 in recognition and signaling in HGECs to express IL-8 upon infection with C. albicans.

In vitro mechanisms of interleukin-8-mediated responses of human gingival epithelial cells to Candida albicans infection

“…5b). Previously, we reported the ICAM-1-dependent activation of IL-8 expression in C. albicans-infected HGECs (Egusa et al, 2005). We investigated the relationship between the ICAM-1-dependent and IL-1a-dependent IL-8 mRNA induction using neutralizing mAbs against ICAM-1 and IL-1a (Fig.…”

“…Previously, we reported that HGECs induce IL-8 production in response to C. albicans infection via ICAM-1 signaling (Egusa et al, 2005). C. albicans expresses on the cell surface b2 integrin-like proteins that are involved in adhesion and morphogenesis (Hostetter, 1999); these C. albicans proteins may bind to ICAM-1 on HGECs as a ligand, resulting in the activation of IL-8 gene transcription (Egusa et al, 2005).…”

“…C. albicans expresses on the cell surface b2 integrin-like proteins that are involved in adhesion and morphogenesis (Hostetter, 1999); these C. albicans proteins may bind to ICAM-1 on HGECs as a ligand, resulting in the activation of IL-8 gene transcription (Egusa et al, 2005). Thus, HGEC production of IL-8 can be induced by at least two distinct mechanisms, one stimulated by IL-1a and the other by ICAM-1 ligation.…”

“…Interleukin-8 (IL-8) is a major constitutive proinflammatory product of oral epithelial cells. During infection with viable C. albicans (Dongari-Bagtzoglou and Kashleva, 2003;Egusa et al, 2005;Mostefaoui et al, 2004;Schaller et al, 2002Schaller et al, , 2004, IL-8 attracts human neutrophils and lamina propria lymphocytes to the site of infection (Bickel, 1993;Schaller et al, 2004) and activates neutrophils and monocytes to kill fungal targets (Djeu et al, 1990). To develop a preventive treatment effective against oral candidiasis, it is necessary to clarify the Candida and host-related pathways inducing the IL-8 response.…”

“…IL-8 production is regulated by cytolytically released interleukin-1a (IL-1a) (DongariBagtzoglou et al, 2004). We recently discovered that human gingival epithelial cells (HGECs) induce IL-8 production in response to C. albicans infection via ligation of a leukocyte adhesion molecule, intercellular adhesion molecule-1 (ICAM-1) (Egusa et al, 2005). The overall profiles of cytokine and inflammatory mediator production by oral epithelial cells in the initial stages of C. albicans infection, however, are not well understood.…”