Intercellular coupling amplifies fate segregation during
            <i>Caenorhabditis elegans</i>
            vulval development

Giurumescu, Claudiu A.; Sternberg, Paul W.; Asthagiri, Anand R.

doi:10.1073/pnas.0506476103

Cited by 52 publications

(42 citation statements)

References 25 publications

(25 reference statements)

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“…The detailed comparison between N2, CB4856, and AB1 gives experimental support to modeling studies, suggesting that a robust signaling network might enable different wild isolates to adopt different sets of parameters (enzymatic activities, protein levels, affinities, etc.) (von Dassow et al 2000;Meir et al 2002;Giurumescu et al 2006). …”

Section: Cryptic Genetic Variation In the Vulval Signaling Network Inmentioning

confidence: 99%

Intraspecific evolution of the intercellular signaling network underlying a robust developmental system

Milloz¹,

Duveau²,

Nuez³

et al. 2008

Genes Dev.

119

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Many biological systems produce an invariant output when faced with stochastic or environmental variation. This robustness of system output to variation affecting the underlying process may allow for "cryptic" genetic evolution within the system without change in output. We studied variation of cell fate patterning of Caenorhabditis elegans vulva precursors, a developmental system that relies on a simple intercellular signaling network and yields an invariant output of cell fates and lineages among C. elegans wild isolates. We first investigated the system's genetic variation in C. elegans by means of genetic tools and cell ablation to break down its buffering mechanisms. We uncovered distinct architectures of quantitative variation along the Ras signaling cascade, including compensatory variation, and differences in cell sensitivity to induction along the anteroposterior axis. In the unperturbed system, we further found variation between isolates in spatio-temporal dynamics of Ras pathway activity, which can explain the phenotypic differences revealed upon perturbation. Finally, the variation mostly affects the signaling pathways in a tissue-specific manner. We thus demonstrate and characterize microevolution of a developmental signaling network. In addition, our results suggest that the vulva genetic screens would have yielded a different mutation spectrum, especially for Wnt pathway mutations, had they been performed in another C. elegans genetic background.[Keywords: Signaling network; evolution; C. elegans; vulva; robustness; cryptic variation] Supplemental material is available at http://www.genesdev.org.

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Section: Cryptic Genetic Variation In the Vulval Signaling Network Inmentioning

confidence: 99%

Intraspecific evolution of the intercellular signaling network underlying a robust developmental system

Milloz¹,

Duveau²,

Nuez³

et al. 2008

Genes Dev.

119

View full text Add to dashboard Cite

show abstract

“…Dans une certaine mesure, un système de spécification cellulaire tel que celui du développement de la vulve peut s'accommoder de variations et cependant produire le même motif final. L'étude in silico de la robustesse et des variations possibles d'un système donne alors beaucoup d'informations [26,27]. …”

Section: Variations Cryptiques Dans La Construction Du Phénotypeunclassified

Variations génétiques et environnementales dans un réseau de signalisation intercellulaire

Félix

2009

Med Sci (Paris)

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Importance de la variation en biologieLes biologistes de laboratoire cherchent le plus souvent à réduire la variabilité dans leurs expériences, se désintéressant de la variation du système qu'ils étudient. Néanmoins, la variance, et plus précisément la distribution des variables, sont des caractéristiques du système, au même titre que les valeurs moyennes de ces variables. Par exemple, si une pathologie apparaît à partir d'un seuil dans la concentration d'une molécule, la distribution de la variation de cette concentration est cruciale pour caractériser le système. La variation entre individus est aussi la base de l'évo-lution. Dans la variation entre individus on distingue : (1) les variations génétiques héréditaires ; (2) les variations environnementales qui forment le contexte écologique dans lequel les organismes se développent et sont sélectionnés ; (3) les variations stochastiques (ou microenvironnementales), que l'on peut révéler lors du développement d'individus génétiquement identiques dans le même environnement [1,2].En ce qui concerne la population humaine, la diversité des conditions de vie et la diversité génétique contribuent conjointement à la variation interindividuelle au cours de l'évolution de l'espèce et dans le contexte médical.

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“…not all possible variations can be explored), and that this approach enables control over large-scale patterns (e.g. transitioning from reflectional to translational symmetry in the linear order of vulval cell types) (Giurumescu et al, 2006). These types of systems-based approaches may be augmented by automated text-mining/curating systems, such as the one described by Andrey Rzhetsky (Columbia University) (Rzhetsky et al, 2004).…”

Section: Does Combining Engineering and Biological Approaches Advancementioning

confidence: 99%

What lies at the interface of regenerative medicine and developmental biology?

Ingber

Levin²

2007

Development

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At a recent Keystone Symposium on `Developmental Biology and Tissue Engineering', new findings in areas ranging from stem cell differentiation,embryonic pattern formation and organ regeneration to engineered cell microenvironments, synthetic biomaterials and artificial tissue fabrication were described. Although these new advances were exciting, this symposium clarified that biologists and engineers often view the challenge of tissue formation from different, and sometimes conflicting, perspectives. These dichotomies raise questions regarding the definition of regenerative medicine,but offer the promise of exciting new interdisciplinary approaches to tissue and organ regeneration, if effective alliances can be established.

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Intercellular coupling amplifies fate segregation during Caenorhabditis elegans vulval development

Abstract: During vulval development in

Cited by 52 publications

References 25 publications

Intraspecific evolution of the intercellular signaling network underlying a robust developmental system

Intraspecific evolution of the intercellular signaling network underlying a robust developmental system

Variations génétiques et environnementales dans un réseau de signalisation intercellulaire

What lies at the interface of regenerative medicine and developmental biology?

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