Interchangeability of Measurements of Total and Free Prostate-Specific Antigen in Serum with 5 Frequently Used Assay Combinations: An Update

Abstract

Background:The comparability of total and free prostate-specific antigen (tPSA and fPSA) results among commercial PSA assays has been suggested to be improved by calibration to WHO PSA reference materials and the development of equimolar-response assays. To characterize the current situation, we assessed 5 frequently used commercial assay combinations for tPSA and fPSA regarding the interchangeability of the PSA values and the ratio of fPSA to tPSA (%fPSA), equimolar characteristics, and diagnostic accuracy.

Androgen deprivation decreases prostate specific antigen in the absence of tumor: implications for interpretation of PSA results

“…PSA was then determined by an electrochemiluminescence immunoassay "ECLIA" on the Roche Elecsys 2010 (Roche Diagnostics GmbH, Mannheim, Germany; intra-assay CV < 3%) [21].…”

An appeal to medical journal editors: the need for a full description of laboratory methods and specimen handling in clinical study reports

“…Although interassay variability among commercially available methods for tPSA and fPSA has improved as a result of using WHO PSA reference materials and developing equimolar-response assays, harmonization among methods is poor, and the interchangeability of tPSA, fPSA, and % fPSA results obtained by various assays is inadequate (11) . As a result, the diagnostic accuracy of prostate cancer risk prediction using a particular risk algorithm depends on the tPSA and fPSA assays used (12) .…”

Biosensors for Cancer Biomarkers

“…Early detection of protein-based biomarkers for biosensor technology in the last few years as it is known to be very beneficial for the early diagnosis of determination. Prostate-specific antigen (PSA), to determine the most common tumor marker is used on prostate cancer (Benson et al, 1992, Bradford et al, 2006Brawer, 1999, Stephan et al, 2006. PSA is a glycoprotein of 32-33 kDa single chain (Landis et al, 1999, Cesar et al, 2004), a part of 93% sugar residue peptide also contains the rest of it is produced by the prostate tissues (Loeb and Cantolona, 2007 ).…”