Low-cost sensors (LCSs) of Geekcreit PM1/PM2.5/PM10 (based on a PMS5003 sampler) and BOHU BH-1 models A3 and B3 (based on a Pando G7 sampler) were compared for different aerosol size ranges using a research-grade instrument (Grimm 1.109) under controlled laboratory conditions. An aerosol generator was utilized to produce various sizes of monodispersed particulate matter (PM), which was introduced into a laboratory smoke chamber under resistance heating/cooling and/or varying RH conditions. In addition, the accuracy of the air temperature (T) and relative humidity (RH) sensors of the LCSs were assessed against calibrated, laboratory-grade instruments. The study LCSs showed generally accurate readings for PM2.5, irrespectively of the slow T and/or RH changes, which provided apt conditions for accurate calibration slopes (S) and low intercepts/bias (b) of the linear fits. On the other hand, PM1 and PM10 readings slightly deviated from those observed with the reference monitor, likely due to the lower detection efficacy of the LCSs towards fine and coarse PM. Varying RH influenced the S and b values, showing its impact on the detection efficacy of LCSs. Under low/medium RH, homoscedastic calibration curves of PMx were found, whereas rather heteroscedastic calibration plots were observed at high RH. For T calibration, low RH in the smoke chamber provided more reproducible conditions in terms of lower measurement bias for LCSs as recorded against a calibrated, reference-grade thermometer.