Interconnectedness of global hemostasis assay parameters in simultaneously evaluated thrombin generation, fibrin generation and clot lysis in normal plasma

Abstract

In normal plasma, TG, FG and FL parameters may provide interchangeable information. Evaluation of FL-resistance may provide additional data under special assay conditions, but the value of this information should be studied under disease conditions.

Development and validation of a high throughput whole blood thrombolysis plate assay

“…measured a time to 50% lysis of around 60 minutes with a t-PA dose of 0.03 µg/mL, and we obtained a T0.5 value of 57.6 ± 13.8 minutes with a t-PA dose of 0.7 nM (i.e. 48 ng/mL) 33 .…”

Thrombin generation abnormalities in Quebec platelet disorder

“…6,12,14 TG assays are traditionally performed without inhibitors of fibrinolysis 1 and some add tPA to simultaneously assess plasmin generation. 22,23 The greater effects of TXA (a lysine analogue that inhibits fibrinolysis) on PRP compared to PPP TG are interesting and could reflect that platelets express uPAR 37 and release polyphosphate that also enhances plasmin generation. 38 It would be interesting to determine if TXA improves or corrects the TG abnormalities of severe PAI-1-deficient samples, 24 in TG assays of PPP and PRP, with or without added uPA and tPA.…”

“…FV is very susceptible to plasmin‐mediated degradation, 36 which occurs in QPD α‐granules 6,12,14 . TG assays are traditionally performed without inhibitors of fibrinolysis 1 and some add tPA to simultaneously assess plasmin generation 22,23 . The greater effects of TXA (a lysine analogue that inhibits fibrinolysis) on PRP compared to PPP TG are interesting and could reflect that platelets express uPAR 37 and release polyphosphate that also enhances plasmin generation 38 .…”

“…20,21 Some fibrinolytic disorders do not alter TG, and adding tissue plasminogen activator (tPA) to normal PPP has minimal effects on TG. 22,23 In TG assays with added tPA, severe PAI-1-deficient PPP shows a shortened lag time, shortened time to peak TG, and reduced peak thrombin concentration that have been attributed to increased activation, and inactivation, of multiple coagulation factors by plasmin. 24 As QPD increases platelet uPA and depletes platelets of active PAI-1, 10 it might similarly alter TG.…”

<b><i>In vitro</i> inactivation of thrombin generation by polysulfated fractions isolated from the tropical coenocytic green seaweed <i>Caulerpa racemosa</i> (Caulerpaceae, Bryopsidales)

“…Biological Sciences Maringá, v. 39, n. 3, p. 283-292, July-Sept., 2017 time (PT), respectively, although emphasizing that these two in vitro tests do not reflect the hemostasis (Ferreira, Sousa, Dusse, & Carvalho, 2010). Given these limitations and the complexity of the coagulation process, thrombin generation (TG)-based assays would offer a better understanding of the in vivo event to measure TG in a blood plasma sample (Castoldi & Rosing, 2011;Xin, Chang, & Ovanesov, 2016) and analyze anticoagulants Zavyalova & Kopylov, 2016). Circulatory dysfunctions are usually associated with the sedentary lifestyle, poor eating habits and stress that potentially lead to venous thromboembolism, heart attack and pulmonary embolism as the most prevalent cardiovascular diseases worldwide, leading causing to patient death or else partial or total disability.…”