Interconnection between nitric oxide formation and hypersensitivity parameters under guinea pig model of acute asthma with multiple challenges

Abstract

an immunoregulatory role of nitric oxide (NO) in the development of adaptive immune responses associated with allergic diseases is very important. The present study extended these observations by the examination of the reciprocal changes in characteristic immunologic parameters of the disease and NO level of bronchoalveolar lavage (Bal) cells under guinea pig model of acute asthma with multiple challenges. development of guinea pig Th2 mediated asthma was accompanied by increasing the level of allergic markers: ovalbumin (OVA) specific IgG and IL-4. We demonstrated that the infiltrate of airway cells contributes to NO synthesis in the respiratory tract during allergic inflammation. The level of intracellular NO formation significantly correlated with plasma allergen specific IgG value in OVA-induced asthma. The presented data evidence that the elevated intracellular NO level in BAL fluid may reflect a nitrosative stress in respiratory tract in general, when allergic asthma exacerbation is present.

Nitrosative events in atopic asthma pathogenesis

“…To give insight into the role of nitrosative stress established by immune cells infiltrating the airways we examined the NO formation in BAL cells under the guinea pig model of acute ovalbumin (OVA)induced asthma with repeated challenges following 32 weeks after sensitization [73]. This model mimics those seen in humans exposed to an allergen a long period after acquired hypersensitivity.…”

“…NO formation as a function of cellular size in BALF subpopulations derived from OVA sensitized guinea pigs (I column) and OVA sensitized animals treated with OVA aerosol exposures (II column). DAF-2DA stained cells are colored in green (adopted from [73] and supplemented here) Fig. 5.…”

“…NO generation in accordance with the complexity of BALF cell subpopulations of OVA sensitized guinea pigs (I column) and of OVA sensitized animals treated with OVA aerosol exposures (II column). DAF-2DA stained cells are colored in green (adopted from [73] and supplemented here) [75]. In allergic asthma the immune response to inhaled antigens results from the activation of mast cells and antigen-specific Th2 cells, followed by the production of cytokines, including interleukin IL-4, IL-5 and IL-13 [3].…”