Interconnections between meiotic recombination and sequence polymorphism in plant genomes

Ziolkowski, Piotr A; Henderson, Ian

doi:10.1111/nph.14265

Cited by 16 publications

(13 citation statements)

References 78 publications

(161 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further, our study of hybrid 3 intervals interactions indicated that the contribution of the cis effects was two times larger than that of trans effects when it comes to explaining the variance of recombination rate. Our results altogether suggest that heterozygosity alone is not sufficient to explain the variation observed in CO numbers and positions across hybrids, in accordance with previous works (Salomé et al 2012;Bauer et al 2013;Rodgers-Melnick et al 2015;Ziolkowski and Henderson 2017). CO control may also depend on other factors such as structural differences between homologous genomes that can inhibit CO formation as observed in A. thaliana (Fransz et al 2016), or modify CO frequency as suggested in maize (Bauer et al 2013;Rodgers-Melnick et al 2015).…”

Section: Genetic Control Of Recombination Ratesupporting

confidence: 89%

Role of Cis, Trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae

et al. 2018

View full text Add to dashboard Cite

Meiotic recombination is a major driver of genome evolution by creating new genetic combinations. To probe the factors driving variability of meiotic recombination, we used a high-throughput method to measure recombination rates in hybrids between SK1 and a total of 26 Saccharomyces cerevisiae strains from different geographic origins and habitats. Fourteen intervals were monitored for each strain, covering chromosomes VI and XI entirely, and part of chromosome I. We found an average number of crossovers per chromosome ranging between 1.0 and 9.5 across strains ("domesticated" or not), which is higher than the average between 0.5 and 1.5 found in most organisms. In the different intervals analyzed, recombination showed up to ninefold variation across strains but global recombination landscapes along chromosomes varied less. We also built an incomplete diallel experiment to measure recombination rates in one region of chromosome XI in 10 different crosses involving five parental strains. Our overall results indicate that recombination rate is increasingly positively correlated with sequence similarity between homologs (i) in DNA doublestrand-break-rich regions within intervals, (ii) in entire intervals, and (iii) at the whole genome scale. Therefore, these correlations cannot be explained by cis effects only. We also estimated that cis and trans effects explained 38 and 17%, respectively, of the variance of recombination rate. In addition, by using a quantitative genetics analysis, we identified an inbreeding effect that reduces recombination rate in homozygous genotypes, while other interaction effects (specific combining ability) or additive effects (general combining ability) are found to be weak. Finally, we measured significant crossover interference in some strains, and interference intensity was positively correlated with crossover number.

show abstract

Section: Genetic Control Of Recombination Ratesupporting

confidence: 89%

Role of Cis, Trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae

et al. 2018

View full text Add to dashboard Cite

show abstract

“…This points to the presence of trans acting factors modulating CO formation, in addition to possible cis effects. Our results altogether suggest that heterozygosity alone is not sufficient to explain the variation observed in CO numbers and positions across hybrids, as previously reported [8,58–60,60]. CO control may also depend on other factors such as structural differences between homologous genomes that can (1) inhibit CO formation as observed in A. thaliana [61], or (2) modify CO frequency as suggested in maize [8,58].…”

Section: Discussionsupporting

confidence: 67%

Role ofcis,trans, and inbreeding effects on meiotic recombination inSaccharomyces cerevisiae

Raffoux

Bourge

Dumas

et al. 2018

Preprint

View full text Add to dashboard Cite

15Meiotic recombination is a major driver of genome evolution by creating new genetic combinations. 16To probe the factors driving variability of meiotic recombination, we used a high-throughput method 17 to measure recombination rates in 26 S. cerevisiae strains from different geographic origins and 18 habitats. Fourteen intervals were monitored for each strain, covering chromosomes VI and XI entirely, 19and part of chromosome I. We found an average number of crossovers per chromosome ranging 20 between 1.0 and 9.5 across strains ("domesticated" or not), which is higher than the average 21 between 0.5 and 1.5 found in most organisms. In the different intervals analyzed, recombination 22showed up to 9-fold variation across strains but global recombination landscapes along 23 chromosomes varied less. We also built an incomplete diallel experiment to measure recombination 24 rates in one region of chromosome XI in 10 different crosses involving five parental strains. Our 25overall results indicate that recombination rate is increasingly positively correlated with sequence 26. CC-BY 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/361428 doi: bioRxiv preprint first posted online Jul. 3, 2018; 2 similarity between homologs (i) in DSB rich regions within intervals, (ii) in entire intervals, and (iii) at 27 the whole genome scale. Therefore, these correlations cannot be explained by cis-effects only. In 28 addition, by using a quantitative genetics analysis, we identified an inbreeding effect that reduces 29 recombination rate in homozygous genotypes while other interaction effects (specific combining 30 ability) or additive effects (general combining ability) are found to be weak. Finally, we measured 31 significant crossover interference in some strains, and interference intensity was positively correlated 32 with crossover number. 44Using a high throughput method based on fluorescent markers, we investigated the diversity of 45 recombination in the budding yeast Saccharomyces cerevisiae. We observed up to 9-fold differences 46 in numbers of crossovers across hybrids obtained by crossing different strains with a common tester, 47and this variation was correlated with the degree of DNA sequence similarity between homologous 48 chromosomes. By also investigating homozygotes, we conclude that on the one hand too much 49 sequence divergence impairs recombination in distantly-related hybrids, and on the other hand 50 complete homozygosity is also associated with lower numbers of crossovers.

show abstract

“…Following the seminal works of Ross et al ( 1997 ) and Klimyuk and Jones ( 1997 ), great strides have been made in elucidating the basic molecular mechanisms of meiosis and meiotic recombination in plants. As a series of recent reviews have provided comprehensive insights into these processes (Lambing et al 2017 ; Lambing and Heckmann 2018 ; Lawrence et al 2017 ; Mercier et al 2015 ; Mézard et al 2015 ; Ziolkowski and Henderson 2017 ), we will only briefly outline some key aspects here.…”

Section: Control Of Co Formation In Plantsmentioning

confidence: 99%

“…It has long been observed that CO frequencies tend to decrease between regions with increased sequence divergence (Borts and Haber 1987 ; Liharska et al 1996 ; Ziolkowski and Henderson 2017 ). Local inhibition of recombination due to sequence divergence is usually attributed to the presence of mismatched base pairs within recombination intermediates.…”

Section: Control Of Co Formation In Plantsmentioning

confidence: 99%

Manipulation of crossover frequency and distribution for plant breeding

Blary

Jenczewski

2018

Theor Appl Genet

View full text Add to dashboard Cite

The crossovers (COs) that occur during meiotic recombination lead to genetic diversity upon which natural and artificial selection can act. The potential of tinkering with the mechanisms of meiotic recombination to increase the amount of genetic diversity accessible for breeders has been under the research spotlight for years. A wide variety of approaches have been proposed to increase CO frequency, alter CO distribution and induce COs between non-homologous chromosomal regions. For most of these approaches, translational biology will be crucial for demonstrating how these strategies can be of practical use in plant breeding. In this review, we describe how tinkering with meiotic recombination could benefit plant breeding and give concrete examples of how these strategies could be implemented into breeding programs.

show abstract

Interconnections between meiotic recombination and sequence polymorphism in plant genomes

Cited by 16 publications

References 78 publications

Role of Cis, Trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae

Role of Cis, Trans, and Inbreeding Effects on Meiotic Recombination in Saccharomyces cerevisiae

Role ofcis,trans, and inbreeding effects on meiotic recombination inSaccharomyces cerevisiae

Manipulation of crossover frequency and distribution for plant breeding

Contact Info

Product

Resources

About