The InterCriteria decision-making approach, known as InterCriteria analysis (ICrA), was applied here to optimize the experimental protocol when the surface electromyography (EMG) signals of upper arm human muscles are recorded. Ten healthy subjects performed cycling movements in the sagittal plane with and without added weight for ten, six, two, and one second, respectively, for each active phase. The EMG signals from six muscles or parts of muscles, namely m. deltoideus pars clavicularis and pars spinata, m. brachialis, m. anconeus, m. biceps brachii, and m. triceps brachii caput longum, were recorded. ICrA was used on the obtained data to find correlations between the sixteen different phases, eight for elbow flexion and eight for elbow extension. Based on the obtained results, we proposed an optimized experimental protocol (OEP) that omits slower and more difficult tasks while saving crucial data. The optimized protocol consists of seven, instead of ten, tasks and takes three minutes less than the time taken for the full experimental protocol (FEP). The lower number of movements in the OEP could prevent physical and psychical fatigue, discomfort, or even pain in the investigated subjects. In addition, the time to train subjects, as well as the time to process the surface EMG data, can be significantly reduced.