Interdigitated Array Microelectrode-Based Electrochemical Impedance Immunosensor for Detection of Escherichia coli O157:H7

Yang, Liju; Li, Yanbin; Erf, Gisela F.

doi:10.1021/ac0352575

Cited by 430 publications

(235 citation statements)

References 32 publications

Supporting

Mentioning

228

Contrasting

Unclassified

Order By: Relevance

“…In general, the technique has been applied to detecting and/or quantifying food-borne microorganisms [1]. Recently, it has received attention from researchers in other fields due to its ability of rapid measurement for bacterial concentrations [4][5][6][7]. In some studies, the dielectrophoretic impedance measurement method was combined with antigen-antibody reaction [6,8,9].…”

mentioning

confidence: 99%

“…Recently, it has received attention from researchers in other fields due to its ability of rapid measurement for bacterial concentrations [4][5][6][7]. In some studies, the dielectrophoretic impedance measurement method was combined with antigen-antibody reaction [6,8,9]. In other studies, the electrical impedance measurements were used to detect the ionic metabolites produced during the bacterial growth [10,11].…”

mentioning

confidence: 99%

See 1 more Smart Citation

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C 1 + C 2 × e (−X/C 3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C 1−3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

show abstract

mentioning

confidence: 99%

mentioning

confidence: 99%

Development of a novel conductance-based technology for environmental bacterial sensing

Shen

Tan

et al. 2013

Chin. Sci. Bull.

View full text Add to dashboard Cite

show abstract

“…The integration of impedance technique with biosensor technology has led to the recent development of impedance biosensors that is expending rapidly for bacteria detection (Ruan et al, 2002;Yang et al, 2004b;Radke and Alocilja 2005). Impedance biosensors for bacteria detection are based on impedance analysis of the electrical properties of bacterial cells when they are attached to or associated with the electrodes.…”

Section: Role Of Impedance Techniques For Bacteria Detectionmentioning

confidence: 99%

“…For example, micrometer IDA electrodes are suitable for sensing of biological cells, while nanometer IDAs are more proper for sensing DNA. Yang et al (2004b) reported a label-free electrochemical impedance immunosensors for detection of E. coli O157:H7 using interdigitated microelectrodes. The IDA consisted of a pair of microband array indium-tin oxide (ITO) electrodes.…”

Section: Electrochemical Impedance Biosensors Using Redox Probesmentioning

confidence: 99%

Food‐borne Pathogenic Bacteria

Shi

Xie

Zhou

2017

Food Safety in China

View full text Add to dashboard Cite

The realization of rapid, sensitive, and specific methods to detect foodborne pathogenic bacteria is central to implementing effective practice to ensure food safety and security. As a principle of transduction, the impedance technique has been applied in the field of microbiology as a means to detect and/or quantify foodborne pathogenic bacteria. The integration of impedance with biological recognition technology for detection of bacteria has led to the development of impedance biosensors that are finding wide-spread use in the recent years. This paper reviews the progress and applications of impedance microbiology for foodborne pathogenic bacteria detection, particularly the new aspects that have been added to this subject in the past few years, including the use of interdigitated microelectrodes, the development of chip-based impedance microbiology, and the use of equivalent circuits for analysis of the impedance systems. This paper also reviews the significant developments of impedance biosensors for bacteria detection in the past 5 years, focusing on microfabricated microelectrodes-based and microfluidic-based Faradaic electrochemical impedance biosensors, non-Faradaic impedance biosensors, and the integration of impedance biosensors with other techniques such as dielectrophoresis and electropermeabilization. Published by Elsevier Inc.

show abstract

“…Yang et al described a label-free impedance immunosensor for the rapid detection of Escherichia coli O157:H7 by immobilizing anti-E. coli antibodies onto an indium-tin oxide interdigitated array (IDA) microelectrode. 162 The authors showed that the immobilization of antibodies and the binding of E. Coli cells to the IDA microelectrode surface increased the electron-transfer resistance, which was directly measured with electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3-/4-as a redox probe. The electron-transfer resistance was correlated with the concentration of E. Coli cells in a range from 10 5 to 10 8 cfu/mL with the detection limit of 10 6 cfu/mL which was comparable with other label free electrochemical immunoassays.…”

Section: Other Immunosensorsmentioning

confidence: 99%