Intérêt de la méthode ELISA dans le diagnostic des leptospiroses

Mailloux, M; Dufresne, Yoann; Mazzonelli, J.; Dorta, G; Mazzonelli,

doi:10.1016/s0399-077x(84)80201-2

Cited by 7 publications

(3 citation statements)

References 6 publications

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“…16 Over the last three decades, infectious disease physicians from Marseille have reported the epidemiology, clinical course, and treatment of leptospirosis in France, French overseas territories, Indonesia, and in travelers from endemic areas. 15,19,22,29 The constant focus of infectious disease physicians in Marseille on leptospirosis has minimized misdiagnoses, in spite of atypical clinical manifestations. 16,30 Therefore, we believe that the emergence of leptospirosis is not related to a change in disease surveillance or the awareness of physicians.…”

Section: Discussionmentioning

confidence: 99%

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Strikes, flooding, rats, and leptospirosis in Marseille, France

Socolovschi

Angelakis

Renvoisé

et al. 2011

International Journal of Infectious Diseases

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Section: Discussionmentioning

confidence: 99%

“…last 30 years. [18][19][20][21] In 1984, three cases of leptospirosis occurred near Marseille, and two cases were imported from the Union of the Comoros and Singapore. 15 Later, one additional case of imported leptospirosis from Spain was reported.…”

Section: Introductionmentioning

confidence: 99%

Strikes, flooding, rats, and leptospirosis in Marseille, France

Socolovschi

Angelakis

Renvoisé

et al. 2011

International Journal of Infectious Diseases

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“…Cases were identified using the Leptospirosis Registry at the Department of Bacteriology, Hôpital Sud, Rennes. For a case to qualify as a confirmed case of leptospirosis, all three of the following criteria were required: (1) a clinically compatible illness; (2) the presence of thermo‐resistant antigen [6] or an IgM antibody titre (ELISA) of at least 1:400 [7]; and (3) a positive microscopic agglutination test result, with a titre of at least 1:100 [8]. These criteria are considered to be indicative of recent or current leptospirosis in areas with a low incidence [2].…”

Section: Methodsmentioning

confidence: 99%

Clinical presentation of leptospirosis: a retrospective study of 34 patients admitted to a single institution in metropolitan France

Jauréguiberry

Roussel

Brinchault-Rabin

et al. 2005

Clinical Microbiology and Infection

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Leptospirosis has a highly variable clinical presentation, which may be related to different infecting serovars, host factors, or a combination of these. This study investigated retrospectively 34 consecutive patients with serologically confirmed leptospirosis admitted during the period 1992-2002. On admission, the most frequent symptoms were fever (100%), headache (75%), myalgia (55%), arthralgia (45%) and vomiting (39%). Pertinent laboratory findings included lymphopenia (85%), thrombocytopenia (75%), elevated liver enzymes (87%) and renal abnormalities (proteinuria, 77%; haematuria, 58%; elevated serum creatinine, 53%). The study confirmed the variable clinical and biological symptoms of leptospirosis, and indicated that lymphopenia is a common feature of leptospirosis cases.

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Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis

Smits

Eapen

Sugathan

et al. 2001

Clin Diagn Lab Immunol

122

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An assay device for the rapid detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera is presented. The sensitivity (85.8%) and specificity (93.6%) of the assay compared well (91.9% agreement) with those of an IgM enzyme-linked immunosorbent assay routinely used in the serodiagnosis of leptospirosis. The sensitivity of the assay varied with the stage of the disease. The assay uses stabilized components and is simply performed by the addition of serum and sample fluid to the sample well of the assay device. The assay is read after 10 min, and a positive result is obtained when staining of the test line is observed.As the clinical symptoms and signs of leptospirosis often are nonspecific, the disease is easily mistaken for other major infectious diseases. Manifestations of leptospirosis may vary, and different types of disease may be observed, from relatively mild influenza-like symptoms to severe disease with renal failure, liver impairment, and haemorrhage (Weil's syndrome). Meningismus and (aseptic) meningitis can be observed as well. Because of the wide variety of symptoms, leptospirosis is easily confused with many other fibril illnesses including haemorrhagic fevers, e.g., dengue fever (7). Laboratory testing to confirm the clinical diagnosis thus is essential for optimal treatment and patient management. The laboratory diagnosis of leptospirosis mainly depends on serology (8). The microscopic agglutination test (MAT) (5, 31) is considered the reference test for leptospirosis, but the enzyme-linked immunosorbent assay (ELISA) (2, 15-17, 19, 32, 34, 36) and a number of other tests including the immunofluorescent-antibody test (IFAT) (3), the slide agglutination test (9), the macrocapsule agglutination test (4), and the hemagglutination test (13,28,29) can be used as well. Drawbacks of the standard diagnostic assays like MAT, ELISA, and IFAT are that they are not very easy to perform, require special and expensive equipment, depend on the availability of electricity and refrigeration, or can be applied only by trained personnel. Hence, these assays are available only in a few specialized laboratories. MAT, which is considered the reference test for leptospirosis, is rarely performed by routine diagnostic laboratories.Leptospirosis has been reported from countries all over the world (1). Sporadic cases of leptospirosis may occur in countries with moderate climates. The disease, however, can be endemic in countries with wet and warm climates. People living under poor socioeconomical and hygienic conditions are at particular risk of getting the disease. Outbreaks have been reported (6, 11, 12, 14, 20-23, 27, 30, 33). Most people at risk cannot depend on health care facilities supported by laboratories capable of performing the more complicated standard laboratory assays. We previously developed a dipstick assay for the detection of Leptospira-specific immunoglobulin M (IgM) antibodies in human sera (10,20,(24)(25)(26)35). This assay can be used outside the specialized laboratory and may e...

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Intérêt de la méthode ELISA dans le diagnostic des leptospiroses

Cited by 7 publications

References 6 publications

Strikes, flooding, rats, and leptospirosis in Marseille, France

Strikes, flooding, rats, and leptospirosis in Marseille, France

Clinical presentation of leptospirosis: a retrospective study of 34 patients admitted to a single institution in metropolitan France

Lateral-Flow Assay for Rapid Serodiagnosis of Human Leptospirosis

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