Photostimulated association and dissociation of anti-dinitrophenyl antibody, anti-DNP-Ab, to and from
a dinitrospiropyran photoisomerizable monolayer is probed by surface plasmon resonance (SPR). The
anti-DNP-Ab binds to a dinitrospiropyran antigen monolayer, whereas the DNP-Ab dissociates from the
protonated dinitromerocyanine monolayer. Reversible binding and dissociation of anti-DNP-Ab to and
from the photoisomerizable monolayer was assayed by SPR spectroscopy. Analysis of the SPR data indicates
that the average thickness of the antibody layer is ca. 70 Å which corresponds to a surface coverage of ca.
4.4 × 10-9 mol·cm-2.