Interfaces, across which fluid and flow properties change significantly, are a ubiquitous feature of most turbulent flows and are present within jets, plumes, homogeneous turbulence, oceans and planetary atmospheres. Even when the interfaces occupy a small volume fraction of the entire flow, they largely control processes such as entrainment and dissipation and can act as barriers to transport. This Theme Issue brings together some of the leading recent developments on interfaces in turbulence, drawing in many methodologies, such as experiments, direct number simulations, inverse methods and analytical modelling.