Mössbauer spectroscopy is frequently applied to gain information on the interfaces in multilayers, and recently the layer sequence permutation of multitrilayers was suggested to enhance its capability to characterize bottom and top interfaces of a given layer. The sequence permutation, however, in the investigated Fe-BAg multitrilayers affected the waviness of the layers as well, and the results hinted at the possibility that although Ag does not mix with Fe and B, the Fe-B amorphous alloy formation is influenced by the layer-sequence-dependent morphology of the Ag layers in the multitrilayers. Now we examine the interface mixing of Fe and B in the case of a fixed layer sequence and varying thickness of the Ag layers in [2 nm B/2 nm Fe/x nm Ag] 4 , 0.2 x 10, multitrilayer samples and in B/Fe/B and B/Fe/Ag trilayers. Below x = 5 nm, both the ratio of the nonalloyed Fe layer and the average hyperfine field of the amorphous Fe-B interface compound change. The variation is attributed to thickness-dependent discontinuities of the Ag layers. Ag acts as a barrier to the diffusion of B from the top side and thus the discontinuities lead to a varying ratio of top and bottom interfaces. The evaluation based on this model shows that the "B on top of Fe" interface has an average B concentration about 11 at. % higher than the "Fe on top of B" interface. A slightly smaller difference, 6 at. %, is deduced from low-temperature conversion electron Mössbauer spectroscopy measurements of the trilayers.