Interfaces with Tunable Mechanical and Radiosensitizing Properties

Berg, Nora G.; Pearce, Brady L.; Snyder, Patrick J.; Rohrbaugh, Nathaniel; Nolan, Michael W.; Adhikari, Prajesh; Khan, Saad A.; Ivanisevic, Albena

doi:10.1021/acsami.5b11639

Cited by 5 publications

(9 citation statements)

References 31 publications

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“…AB & ROS Cellular Assays : The cell viability and ROS assays in the presence of the semiconductor samples were done based on Berg et al Cell viability was assessed using the Alamar Blue assay. In addition to day 0, AB was measured at days 1, 3, and 5.…”

Section: Methodsmentioning

confidence: 99%

Persistent Photoconductivity, Nanoscale Topography, and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Bandgap Semiconductor Surfaces

Snyder

Kirste

Collazo

et al. 2017

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Wide bandgap semiconductors such as gallium nitride (GaN) exhibit persistent photoconductivity properties. The incorporation of this asset into the fabrication of a unique biointerface is presented. Templates with lithographically defined regions with controlled roughness are generated during the semiconductor growth process. Template surface functional groups are varied using a benchtop surface functionalization procedure. The conductivity of the template is altered by exposure to UV light and the behavior of PC12 cells is mapped under different substrate conductivity. The pattern size and roughness are combined with surface chemistry to change the adhesion of PC12 cells when the GaN is made more conductive after UV light exposure. Furthermore, during neurite outgrowth, surface chemistry and initial conductivity difference are used to facilitate the extension to smoother areas on the GaN surface. These results can be utilized for unique bioelectronics interfaces to probe and control cellular behavior.

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Section: Methodsmentioning

confidence: 99%

Persistent Photoconductivity, Nanoscale Topography, and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Bandgap Semiconductor Surfaces

Snyder

Kirste

Collazo

et al. 2017

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“…33 GaOOH/Matrigel composites have been used to modulate the behavior of cells on surfaces with variable stiffness containing a radiosensitizing material. 34 Such studies allow one to understand how to enhance the biological response on a matrix with variable stiffness under therapeutically relevant conditions such as X-ray radiation. Gallium compounds have shown photocatalytic activity, bioavailability and Ga 3+ ions are antiproliferative to pathologically proliferating cells such as cancer cells.…”

Section: ■ Introductionmentioning

confidence: 99%

“…The possibility of surface functionalization with a short-lived radiotracer such as 68 Ga may also allow for positron emission tomography of cancer cells . GaOOH/Matrigel composites have been used to modulate the behavior of cells on surfaces with variable stiffness containing a radiosensitizing material . Such studies allow one to understand how to enhance the biological response on a matrix with variable stiffness under therapeutically relevant conditions such as X-ray radiation.…”

Section: Introductionmentioning

confidence: 99%

Room Temperature Electrochemical Synthesis of Crystalline GaOOH Nanoparticles from Expanding Liquid Metals

2018

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Gallium oxyhydroxide (GaOOH) is a wide band gap semiconductor of interest for a variety of applications in electronics and catalysis where the synthesis of the crystalline form is usually achieved via hydrothermal routes. Here we synthesize GaOOH via the electrochemical oxidation of gallium based liquid metals in solutions of 0.1 M NaNO electrolyte with pH adjusted over the range of 7-8.4 with NaOH. This electrochemical approach employed under ambient conditions results in the formation of crystalline oblong shaped α-GaOOH nanoparticles from both liquid gallium and liquid galinstan which is a eutectic based on Ga, In, and Sn. The size and shape of the GaOOH particles could be controlled by the solution pH. The product is characterized with scanning electron microscopy, transmission electron microscopy, X-ray diffraction, UV-visible spectroscopy, and photoluminescence spectroscopy. During the electrochemical oxidation process, the liquid metal drop was found to expand significantly in the case of galinstan due to a constant electrowetting effect which resulted in the continuous expulsion of nanomaterial from the expanding liquid metal droplet. This electrochemical approach may be applicable to other liquid metals for the fabrication of metal oxide nanomaterials and also demonstrates that significant chemical reactions may be occurring at the surface of liquid metals that are actuated under an applied electric field in aqueous electrolytes.

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“…We note that in prior work we have also assessed the Ga toxicity using an extremely water-soluble salt: Ga(NO 3 ) 3 . 18 Previously, we recorded no concentration dependence on toxicity. In this work, we test the effects of the collective behavior hypothesized to contribute to the production of more ROS and result in an enhanced radiosensitization.…”

Section: Introductionmentioning

confidence: 95%

“…The current work builds on prior promising results that demonstrated that the presence of a scintillator material inside a scaffold triggered different cellular responses when radiation was used. 17 , 18 These studies performed by Berg et al have demonstrated the behavior of GaOOH nanoparticles in cell free environments of both water and cell growth media. We now study the effect of GaOOH particle anisotropy in a composite.…”

Section: Introductionmentioning

confidence: 99%

Ga Ion-Enhanced and Particle Shape-Dependent Generation of Reactive Oxygen Species in X-ray-Irradiated Composites

2018

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The reported results test the effects of the collective behavior hypothesized to contribute to the production of more reactive oxygen species (ROS) in vitro and result in an enhanced radiosensitization. The role of particle shape in composites with gallium oxyhydroxide (GaOOH) particles and Matrigel is studied. Particles of two different shapes are embedded into the gel to understand only the materials effect on the generation of ROS rather than cell penetrating variations. The paper reports materials characterization by scanning electron microscopy and X-ray diffraction. The stability of the particles within the composite is assessed by quantification of leached metal using inductively coupled plasma mass spectrometry. The amount of ROS in each construct under variable radiation conditions is quantified in the presence and absence of PC12 cells seeded on top of the composites. The viability of cells is also recorded under different in vitro conditions. The collective materials characterization and the results from the bioassays are used to explain the role of anisotropy on the radiosensitization of nanostructures containing Ga. The presence of Ga ions in composites can have a radiosensitizing effect, and the amount of the available Ga3+ determines the magnitude of the radiosensitization. The shape of the particles determines the stability in aqueous solutions and release of Ga3+ that triggers ROS production. The concentration and shape of Ga-containing materials can be combined to generate an additive effect by increasing the amount of available free metal ions in solution. The studies with GaOOH containing composites enable one to explore the role of key parameters that lead to an increased efficiency of radiation treatments.

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Interfaces with Tunable Mechanical and Radiosensitizing Properties

Cited by 5 publications

References 31 publications

Persistent Photoconductivity, Nanoscale Topography, and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Bandgap Semiconductor Surfaces

Persistent Photoconductivity, Nanoscale Topography, and Chemical Functionalization Can Collectively Influence the Behavior of PC12 Cells on Wide Bandgap Semiconductor Surfaces

Room Temperature Electrochemical Synthesis of Crystalline GaOOH Nanoparticles from Expanding Liquid Metals

Ga Ion-Enhanced and Particle Shape-Dependent Generation of Reactive Oxygen Species in X-ray-Irradiated Composites

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