Interfacial Assembly of Signal Amplified Multienzymes and Biorecognized Antibody into Proteinosome for an Ultrasensitive Immunoassay

Yan, Xu; Wang, Tingting; Yao, Dong; Xu, Jing; Luo, Quan; Liu, Junqiu

doi:10.1002/smll.201900350

Cited by 35 publications

(20 citation statements)

References 29 publications

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Section: Resultsmentioning

confidence: 99%

“…Selective recognition ability is a vital feature in assuring recognition accuracy and reducing cross-activity of the sensing strategy, especially for constructing an enzyme-triggered biosensor. 44 According to the detection principle, the h-CDs/DA sensor was only supposed to be responsive for AChE inhibitors. To simulate the practical application environment for screening OPs, the effect of the non-OPs (arylpyrrole pesticide, neonicotinoid pesticide, and pyrethroid pesticide) was systematically explored for assessing the specificity.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Design of Red Emissive Carbon Dots: Robust Performance for Analytical Applications in Pesticide Monitoring

Gao

et al. 2020

Anal. Chem.

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146

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Synthesis of red emissive carbon dots (CDs) is highly desirable for sensing applications, as they still remain as bottlenecks in terms of precursor synthesis and product purification. Herein, we have designed a new strategy for realizing efficient red emissive CD optimal emission at 610 nm (fluorescence quantum yield ca. 24.0%) based on solvothermal treatment of citric acid and thiourea using dimethylformamide as solvent. Further investigations reveal that the conjugating sp2-domain controlling the incorporation of nitrogen and surface engineering are mainly responsible for the obtained red emission of CDs. Taking advantage of optical properties and abundant surface functional groups, CDs were considered to facilely construct a ratiometric fluorescent platform for quantifying trace levels of organophosphorus pesticides (OPs). Combining the acetylcholinesterase-mediated polymerization of dopamine and the inhibition of pesticide toward the enzyme, the degree of polymerization of dopamine rationally depends on the concentration of OPs. By measuring the fluorescence intensity ratio, the proposed platform exhibited highly selective and robust performance toward OPs, displaying ultrasensitive recognition in the pg L–1 level. The multiexcitation format could efficiently shield background interference from complex samples by introducing a self-calibrated reference signal, which affords accurate and reliable quantitative information, endowing CDs as a universal candidate for a biosensing application by combining target-specific recognition elements.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Results and Discussionmentioning

confidence: 99%

Design of Red Emissive Carbon Dots: Robust Performance for Analytical Applications in Pesticide Monitoring

Gao

et al. 2020

Anal. Chem.

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146

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“…In consideration of the wide application of the polymer capsules in bio-related fields, more and more biohybrid polymers, especially the protein–polymer biohybrids, are fabricated to construct proteinosomes with better biocompatibility and wider applications compared with the polymer capsules. − For example, Huang and co-workers synthesized proteinosomes by cross-linking the self-assemblies of bioconjugates of the bovine serum albumin (BSA) and poly( N -isopropylacrylamide) (PNIPAM) at the interfaces of emulsion droplets . The proteinosome had protocell properties and could be applied in various fields.…”

Section: Introductionmentioning

confidence: 99%

Stimuli-Responsive Proteinosomes Based on Biohybrid Shell Cross-Linked Micelles

Zhang

Yang

et al. 2021

Langmuir

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A new method of stimuli-responsive proteinosome fabrication with the shell cross-linked micelle as a template is reported in this research. A thermoresponsive diblock copolymer poly[di(ethylene glycol) methyl ether methacrylate]-b-poly[poly(ethylene glycol) methyl ether methacrylate-co-pyridyl disulfide methacrylamide] [PDEGMA-b-P(PEGMA-co-PDSMA)] was synthesized and self-assembled into micelles with PDEGMA cores and P(PEGMA-co-PDSMA) shells at the temperature above its lower critical solution temperature (LCST). Reduced bovine serum albumin (BSA) molecules with six thiol groups were used to cross-link the shells of the micelles by reacting with the pendant pyridyl disulfide groups on the P(PEGMA-co-PDSMA) block. At a temperature below the LCST of the polymer, the PDEGMA cores were dissolved in water, affording proteinosomes with a size of about 50 nm and capsule-like structures. The proteinosome was also thermoresponsive with a phase transition temperature at 35 °C. The fabrication of the proteinosome had no obvious influence on the structure and activity of BSA, and BSA retained most of its secondary structure and esterase-like activity. Because the BSA molecules were connected to the polymer chains through disulfide bonds, they could be released upon addition of dithiothreitol. The in vitro cell viability evaluation and the cellular uptake assay demonstrated that the proteinosome showed low toxicity to NIH 3T3 and 4T1 cells and could be internalized into the 4T1 cells.

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“…[ 3 ] To deeply investigate and profoundly understand the cell structures or functions of such highly orchestrated microstructured assemblies, recently, numerous and various micro‐sized (multi)compartments have been created including polymersomes, [ 4–6 ] liposomes, [ 7 ] colloidosomes, [ 8 ] coacervates, [ 9 ] and proteinosomes, [ 10 ] and other (multi)compartments of them. [ 11–15 ] In order to prepare simplified cellular models certain cellular properties and behaviors have been successfully installed such as, modulated cascade enzymatic reaction, [ 16 ] enhanced protein refolding, [ 17 ] cytoskeletal assembly, [ 18 ] membrane fusion, [ 14,19 ] phagocytosis, [ 20 ] and predatory behavior. [ 21–22 ] However, such simple cell‐sized models are difficult to be used to study a series of complicated biological behaviors and activities.…”

Section: Introductionmentioning

confidence: 99%

Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes‐in‐Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation

Wen

Wang

Moreno

et al. 2020

Small

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The eukaryotic cell is a smart compartment containing an outer permeable membrane, a cytoskeleton, and functional organelles, presenting part structures for life. The integration of membrane‐containing artificial organelles (=polymersomes) into a large microcompartment is a key step towards the establishment of exquisite cellular biomimetics with different membrane properties. Herein, an efficient way to construct a hierarchical multicompartment composed of a hydrogel‐filled proteinosome hybrid structure with an outer homogeneous membrane, a smart cytoskeleton‐like scaffold, and polymersomes is designed. Specially, this hybrid structure creates a micro‐environment for pH‐responsive polymersomes to execute a desired substance transport upon response to biological stimuli. Within the dynamic pH‐stable skeleton of the protein hydrogels, polymersomes with loaded PEGylated insulin biomacromolecules demonstrate a pH‐responsive reversible swelling‐deswelling and a desirable, on‐demand cargo release which is induced by the enzymatic oxidation of glucose to gluconic acid. This stimulus responsive behavior is realized by tunable on/off states through protonation of the polymersomes membrane under the enzymatic reaction of glucose oxidase, integrated in the skeleton of protein hydrogels. The integration of polymersomes‐based hybrid structure into the proteinosome compartment and the stimuli‐response on enzyme reactions fulfills the requirements of eukaryotic cell biomimetics in complex architectures and allows mimicking cellular transportation processes.

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Interfacial Assembly of Signal Amplified Multienzymes and Biorecognized Antibody into Proteinosome for an Ultrasensitive Immunoassay

Abstract: Scheme 1. The general process of proteinosome formation.The authors declare no conflict of interest.

Cited by 35 publications

References 29 publications

Design of Red Emissive Carbon Dots: Robust Performance for Analytical Applications in Pesticide Monitoring

Design of Red Emissive Carbon Dots: Robust Performance for Analytical Applications in Pesticide Monitoring

Stimuli-Responsive Proteinosomes Based on Biohybrid Shell Cross-Linked Micelles

Construction of Eukaryotic Cell Biomimetics: Hierarchical Polymersomes‐in‐Proteinosome Multicompartment with Enzymatic Reactions Modulated Protein Transportation

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