Interfacial energy driven distinctive pattern formation during the drying of blood droplets

Mukhopadhyay, Mainak; Ray, Rudra; Ayushman, Manish; Sood, Pourush; Bhattacharyya, Maitreyee; Sarkar, Debasish; DasGupta, Sunando

doi:10.1016/j.jcis.2020.04.008

Cited by 17 publications

(15 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, the outcome of this research also has the potentiality in applications related to disease diagnosis 22 – 29 . Several studies have revealed that the resulting final patterns of the drying droplets appear correlated to the initial state 11 , 12 .…”

Section: Introductionmentioning

confidence: 97%

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Multi-colloidal systems exhibit a variety of structural and functional complexity owing to their ability to interact amongst different components into self-assembled structures. This paper presents experimental confirmations that reveal an interesting sharp phase transition during the drying state and in the dried film as a function of diluting concentrations ranging from 100% (undiluted whole blood) to 12.5% (diluted concentrations). An additional complementary contact angle measurement exhibits a monotonic decrease with a peak as a function of drying. This peak is related to a change in visco-elasticity that decreases with dilution, and disappears at the dilution concentration for the observed phase transition equivalent to 62% (v/v). This unique behavior is clearly commensurate with the optical image statistics and morphological analysis; and it is driven by the decrease in the interactions between various components within this bio-colloid. The implications of these phenomenal systems may address many open-ended questions of complex hierarchical structures.

show abstract

Section: Introductionmentioning

confidence: 97%

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Pal

Gope

Obayemi

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…During the past decade, significant progress has been observed in the drying droplets of bio-colloids, such as [bovine serum albumin (BSA), and lysozyme] protein [3][4][5][6], plasma [7], DNA [8], serum [9], blood [10], etc., due to biomedical and forensic applications [9,[11][12][13][14][15]. The coupling between the droplet and the substrate, the surrounding environment, and the constituent particles show a wide variability range [16].…”

Section: Introductionmentioning

confidence: 99%

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

2021

View full text Add to dashboard Cite

The drying of bio-colloidal droplets can be used in many medical and forensic applications. The whole human blood is the most complex bio-colloid system, whereas bovine serum albumin (BSA) is the simplest. This paper focuses on the drying characteristics and the final morphology of these two bio-colloids. The experiments were conducted by varying their initial concentrations, and the solutions were dried under various controlled substrate temperatures using optical and scanning electron microscopy. The droplet parameters (the contact angle, the fluid front, and the first-order image statistics) reveal the drying process’s unique features. Interestingly, both BSA and blood drying droplets’ contact angle measurements show evidence of a concentration-driven transition as the behavior changes from non-monotonic to monotonic decrease. This result indicates that this transition behavior is not limited to multi-component bio-colloid (blood) only, but may be a phenomenon of a bio-colloidal solution containing a large number of interacting components. The high dilution of blood behaves like the BSA solution. The ring-like deposition, the crack morphology, and the microstructures suggest that the components have enough time to segregate and deposit onto the substrate under ambient conditions. However, there is insufficient time for evaporative-driven segregation to occur at elevated temperatures, as expected.

show abstract

“…The detection of conformational states of macromolecules is of great relevance for the diagnosis of pathologies such as Huntington’s disease, Parkinson’s disease, prion diseases, and Alzheimer’s disease [ 1 , 3 , 7 ]. Currently, texture analysis of dried droplet deposits of bio-fluids has contributed to the detection of health problems [ 12 , 14 , 15 ]. However, it is unknown whether, through the analysis of the texture of dry droplets, it is possible to detect conformational changes in macromolecules.…”

Section: Discussionmentioning

confidence: 99%

“…Pattern analysis of dried droplets of biofluids is useful for obtaining information about the existence and the structural state of the components of a solution [ 8 , 9 , 10 , 11 ]. For example, a reduction in radial crack lengths in dried droplets of whole blood helps to detect Thalassaemia, a blood disorder where hemoglobin (Hb) is abnormal [ 12 ]. In addition, the length of the large cracks is related to the level of bilirubin in the blood of infants with jaundice [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Patterns in Dried Droplets to Detect Unfolded BSA

Carreón

Gómez-López

Díaz-Hernández

et al. 2022

Sensors

View full text Add to dashboard Cite

The morphological analysis of patterns in dried droplets has allowed the generation of efficient techniques for the detection of molecules of medical interest. However, the effectiveness of this method to reveal the coexistence of macromolecules of the same species, but different conformational states, is still unknown. To address this problem, we present an experimental study on pattern formation in dried droplets of bovine serum albumin (BSA), in folded and unfolded conformational states, in saline solution (NaCl). Folded proteins produce a well-defined coffee ring and crystal patterns all over the dry droplet. Depending on the NaCl concentration, the crystals can be small, large, elongated, entangled, or dense. Optical microscopy reveals that the relative concentration of unfolded proteins determines the morphological characteristics of deposits. At a low relative concentration of unfolded proteins (above 2%), small amorphous aggregates emerge in the deposits, while at high concentrations (above 16%), the “eye-like pattern”, a large aggregate surrounded by a uniform coating, is produced. The radial intensity profile, the mean pixel intensity, and the entropy make it possible to characterize the patterns in dried droplets. We prove that it is possible to achieve 100% accuracy in identifying 4% of unfolded BSA contained in a protein solution.

show abstract

Interfacial energy driven distinctive pattern formation during the drying of blood droplets

Cited by 17 publications

References 34 publications

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Concentration-driven phase transition and self-assembly in drying droplets of diluting whole blood

Temperature and Concentration Dependence of Human Whole Blood and Protein Drying Droplets

Patterns in Dried Droplets to Detect Unfolded BSA

Contact Info

Product

Resources

About