Interfacial phenomena affecting the adhesion of Thiobacillus ferrooxidans to sulphide mineral surface

Solari, Jaime A.; Huerta, G.; Escobar, Beatriz; Vargas, T.; Badilla-Ohlbaum, Ricardo; Rubio, Jorge

doi:10.1016/0166-6622(92)80227-s

Cited by 75 publications

(28 citation statements)

References 18 publications

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“…However, the formation of the biofilm is associated with the metabolic activity of cells attached to the surfaces of the particles. It is believed that the hydrophobicity of the surface of ¹. ferrooxidans cells is the predominant force that influences the adhesion of the cells to particulate surfaces, though electrostatic interactions are also present and affect the adhesion process (DiSpirito et al 1983;Solari et al 1992;Devasia et al 1993). The affinity of ¹. ferrooxidans cells for ferric iron hydrates and jarosites (McGoran et al, 1969;Karamanev 1991), which were observed to accumulate inside the BSP, can also play an important role in immobilization of the cells.…”

Section: Resultsmentioning

confidence: 96%

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Nemati

Webb

1996

Applied Microbiology and Biotechnology

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The kinetics of continuous oxidation of ferrous iron by immobilized cells of ¹hiobacillus ferrooxidans was studied in a packed-bed bioreactor. Polyurethane foam biomass support particles were used as carriers for cell immobilization. Effects of ferrous iron concentration and its volumetric loading on the kinetics of the reaction were investigated. Media containing different concentrations of ferrous iron in the range 5-20 kg m\ were tested. For each medium the kinetics of the reaction at different volumetric loadings of ferrous iron, at a constant temperature of 30°C, were determined. With media containing 5 kg m\ and 10 kg m\ Fe>, the fastest oxidation rates of 34.25 kg m\ h\ and 32 kg m\ h\ were achieved at a dilution rate of around 6 h\, which represents a residence time of 10 min. Employing a higher concentration of ferrous iron (20 kg m\) in the medium resulted in lower oxidation rates, with a maximum value of 10 kg m\ h\, indicating an inhibitory effect of ferrous iron on growth and activity of ¹. ferrooxidans. The reliable performance of the bioreactor during the course of the experiments confirmed the suitability of polyurethane foam biomass support particles as carriers for ¹. ferrooxidans immobilization.

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Section: Resultsmentioning

confidence: 96%

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Nemati

Webb

1996

Applied Microbiology and Biotechnology

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“…Two moles of glucuronic acid residues chelating one mole of iron(III) ions would result in a positively charged complex that might coordinate toward local cathodes at metal sulfide dissolution sites (Gehrke et al 1998). Pyrite has an isoelectric point around 3 and a negative surface charge at lower pH values (Solari et al 1992). The interaction of iron(III) ions complexed by glucuronic acid residues might result in chemical oxidation of iron moieties of the mineral and the formation of a sulfide-containing conditioning layer.…”

Section: Attachment Effectorsmentioning

confidence: 99%

Manipulation of pyrite colonization and leaching by iron-oxidizing Acidithiobacillus species

Bellenberg

Barthen

Boretska

et al. 2014

Appl Microbiol Biotechnol

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In this study, the process of pyrite colonization and leaching by three iron-oxidizing Acidithiobacillus species was investigated by fluorescence microscopy, bacterial attachment, and leaching assays. Within the first 4-5 days, only the biofilm subpopulation was responsible for pyrite dissolution. Pyrite-grown cells, in contrast to iron-grown cells, were able to oxidize iron(II) ions or pyrite after 24 h iron starvation and incubation with 1 mM H₂O₂, indicating that these cells were adapted to the presence of enhanced levels of reactive oxygen species (ROS), which are generated on metal sulfide surfaces. Acidithiobacillus ferrivorans SS3 and Acidithiobacillus ferrooxidans R1 showed enhanced pyrite colonization and biofilm formation compared to A. ferrooxidans (T). A broad range of factors influencing the biofilm formation on pyrite were also identified, some of them were strain-specific. Cultivation at non-optimum growth temperatures or increased ionic strength led to a decreased colonization of pyrite. The presence of iron(III) ions increased pyrite colonization, especially when pyrite-grown cells were used, while the addition of 20 mM copper(II) ions resulted in reduced biofilm formation on pyrite. This observation correlated with a different extracellular polymeric substance (EPS) composition of copper-exposed cells. Interestingly, the addition of 1 mM sodium glucuronate in combination with iron(III) ions led to a 5-fold and 7-fold increased cell attachment after 1 and 8 days of incubation, respectively, in A. ferrooxidans (T). In addition, sodium glucuronate addition enhanced pyrite dissolution by 25%.

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“…Within coal mining regions, oxidation of the mineral, which is facilitated by the presence of microbes, leads to the environmental phenomenon known as acid mine drainage (AMD), that impacts ecosystems primarily through a sulfuric acid laden runoff (Larsson et al, 1993;Nordstrom and Southam, 1997;Edwards et al, 1998;Marchand and Dinkelman, 2006). With an eye toward understanding the chemistry associated with AMD, there has been a significant effort focused on studying the mechanistic details of pyrite oxidation under abiotic and biotic conditions (Shrihari et al, 1991;Solari et al, 1992;Ohmura et al, 1993;Edwards et al, 1999;Elsetinow et al, 2000;Borda et al, 2003;Elsetinow et al, 2003;Hao et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Effects of phospholipid on pyrite oxidation in the presence of autotrophic and heterotrophic bacteria

Hao

Murphy

Lim

et al. 2009

Geochimica et Cosmochimica Acta

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Interfacial phenomena affecting the adhesion of Thiobacillus ferrooxidans to sulphide mineral surface

Cited by 75 publications

References 18 publications

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Effect of ferrous iron concentration on the catalytic activity of immobilized cells of Thiobacillus ferrooxidans

Manipulation of pyrite colonization and leaching by iron-oxidizing Acidithiobacillus species

Effects of phospholipid on pyrite oxidation in the presence of autotrophic and heterotrophic bacteria

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