Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Yang, Sung Ho; Lee, Taemin; Seo, Eunyong; Ko, Eun Hyea; Choi, Insung S.; Kim, Byeong Su

doi:10.1002/mabi.201100268

Cited by 67 publications

(42 citation statements)

References 38 publications

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“…In addition to occupational exposures, GFNs may be deliberately implanted or injected for biomedical applications that include biosensors (10), tissue scaffolds (11,12), carriers for drug delivery (13,14) or gene therapy (15), antibacterial agents (16), and bioimaging probes (17,18). The large specific surface area of graphene allows high-density biofunctionalization or drug loading (19,20) and more efficient tumor targeting capability (13), and graphene may also offer lower toxicity and better manufacturing reproducibility than some other material platforms (18,21).…”

mentioning

confidence: 99%

Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites

Yuan

Bussche

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

679

638

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Understanding and controlling the interaction of graphene-based materials with cell membranes is key to the development of graphene-enabled biomedical technologies and to the management of graphene health and safety issues. Very little is known about the fundamental behavior of cell membranes exposed to ultrathin 2D synthetic materials. Here we investigate the interactions of graphene and few-layer graphene (FLG) microsheets with three cell types and with model lipid bilayers by combining coarse-grained molecular dynamics (MD), all-atom MD, analytical modeling, confocal fluorescence imaging, and electron microscopic imaging. The imaging experiments show edge-first uptake and complete internalization for a range of FLG samples of 0.5-to 10-μm lateral dimension. In contrast, the simulations show large energy barriers relative to k B T for membrane penetration by model graphene or FLG microsheets of similar size. More detailed simulations resolve this paradox by showing that entry is initiated at corners or asperities that are abundant along the irregular edges of fabricated graphene materials. Local piercing by these sharp protrusions initiates membrane propagation along the extended graphene edge and thus avoids the high energy barrier calculated in simple idealized MD simulations. We propose that this mechanism allows cellular uptake of even large multilayer sheets of micrometer-scale lateral dimension, which is consistent with our multimodal bioimaging results for primary human keratinocytes, human lung epithelial cells, and murine macrophages. molecular dynamics simulation | graphene-cell interaction | lipid membrane | edge cutting | corner penetration

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mentioning

confidence: 99%

Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites

Yuan

Bussche

et al. 2013

Proc. Natl. Acad. Sci. U.S.A.

679

638

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“…Up to now, the assembly of a series of different CMGs into the multilayered architectures by LbL assembly has been demonstrated, yielding highly controllable and conformal thin films on different substrates 21, 22, 23, 24, 25. To achieve an effective LbL multilayered assembly, a critical requirement is that two oppositely charged components should be well dispersed in solution.…”

Section: State‐of‐the‐art Self‐assembly Strategies Of Graphene Architmentioning

confidence: 99%

Self‐Assembled Graphene‐Based Architectures and Their Applications

Yuan

Xiao

et al. 2017

Advanced Science

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Due to unique planar structures and remarkable thermal, electronic, and mechanical properties, chemically modified graphenes (CMGs) such as graphene oxides, reduced graphene oxides, and the related derivatives are recognized as the attractive building blocks for “bottom‐up” nanotechnology, while self‐assembly of CMGs has emerged as one of the most promising approaches to construct advanced functional materials/systems based on graphene. By virtue of a variety of noncovalent forces like hydrogen bonding, van der Waals interaction, metal‐to‐ligand bonds, electrostatic attraction, hydrophobic–hydrophilic interactions, and π–π interactions, the CMGs bearing various functional groups are highly desirable for the assemblies with themselves and a variety of organic and/or inorganic species which can yield various hierarchical nanostructures and macroscopic composites endowed with unique structures, properties, and functions for widespread technological applications such as electronics, optoelectronics, electrocatalysis/photocatalysis, environment, and energy storage and conversion. In this review, significant recent advances concerning the self‐assembly of CMGs are summarized, and the broad applications of self‐assembled graphene‐based materials as well as some future opportunities and challenges in this vibrant area are elucidated.

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“…This fluorescence-quenching property of graphene, combined with the interaction between graphene and biomolecules, shows the possibility of developing new types of biosensors. In addition to the properties described above, graphene shows good biocompatibility and therefore has potential applications at the interface between the detection unit and cells [84]- [86].…”

Section: Optical Propertiesmentioning

confidence: 99%

Graphene-Based Glucose Sensors: A Brief Review

Wang

Liu

2015

IEEE Trans.on Nanobioscience

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Abstract-Since the existence of graphene, a material only a single atomic layer thick, was demonstrated about a decade ago, it has caught the attention of researchers worldwide. This paper begins with a historical overview of graphene since its discovery, in 2004, and focuses on a citation-weighted review of graphene-based sensors developed for the detection of biological targets. Based on this statistical analysis, we categorize recent developments in graphene-based biosensors (GBBs) as optimized for detecting 1) proteins, 2) nucleic acids, 3) carbohydrates, or 4) compounds generated by metabolic processes. Existing detection methods employed by these sensors include electrical, electrochemical, and photonic approaches with respect to detecting labeled (or enzyme-assisted) and label-free (or enzyme-free) probe structures. Herein, we focus on graphene-based glucose sensors because glucose-monitoring technology is extremely important in the management of diabetes and many practical examples of these carbohydrate sensors have been developed using the aforementioned detection methods.

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Interfacing Living Yeast Cells with Graphene Oxide Nanosheaths

Cited by 67 publications

References 38 publications

Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites

Graphene microsheets enter cells through spontaneous membrane penetration at edge asperities and corner sites

Self‐Assembled Graphene‐Based Architectures and Their Applications

Graphene-Based Glucose Sensors: A Brief Review

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