Interfacing "Soft" and "Hard" Matter with Exquisite Chemical Control

Woo, Youn-Hi; Camarero, Julio A.

doi:10.2174/157341306776875794

Cited by 12 publications

(8 citation statements)

References 59 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In this review we have tried to show a representative sample of several applications of EPL to study protein structure and function, however the number of applications is starting to grow exponentially. For example, EPL shows special promise in the area of nanotechnology [126], were this mild ligation approach could be used for the site-specific immobilization of proteins onto nanoparticles [127] and solid supports [61]. Another field where EPL would prove increasingly useful is the development of complex protein-based therapeutics.…”

Section: Discussionmentioning

confidence: 99%

Expressed protein ligation: a resourceful tool to study protein structure and function

Berrade

Camarero

2009

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

This review outlines the use of expressed protein ligation (EPL) to study protein structure, function and stability. EPL is a chemoselective ligation method that allows the selective ligation of unprotected polypeptides from synthetic and recombinant origin for the production of semi-synthetic protein samples of well-defined and homogeneous chemical composition. This method has been extensively used for the site-specific introduction of biophysical probes, unnatural amino acids, and increasingly complex post-translational modifications. Since it was introduced 10 years ago, EPL applications have grown increasingly more sophisticated in order to address even more complex biological questions. In this review we highlight how this powerful technology combined with standard biochemical analysis techniques has been used to improve our ability to understand protein structure and function.

show abstract

Section: Discussionmentioning

confidence: 99%

Expressed protein ligation: a resourceful tool to study protein structure and function

Berrade

Camarero

2009

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

show abstract

“…One of the most frequently used strategies to prepare this type of microarray involves the use of monoclonal antibodies as specific protein capture reagents. Antibodies have been classically well suited for this task, since there are a large number of commercially available specific antibodies, which can be easily immobilized onto solid supports (4,(27)(28)(29)(30). However, the potential problems associated with the use of antibodies for chip assembly, which might manifest themselves through moderate expression yields and by issues related to the stability and solubility of these large proteins, have led to the exploration of alternative protein scaffolds as a source for new, more effective and stable protein capture reagents (24,31,32).…”

Section: Protein-detecting Microarraysmentioning

confidence: 99%

“…Key to these methods is the introduction of a unique reacting group at a defined position in the protein to be immobilized, which can later react in a chemoselective manner with a complementary group previously introduced into the surface (Fig. 3 , see also references ( 4 , 27 , 29 , 69 , 78 ) for recent reviews).…”

Section: Novel Approaches For Protein Immobilizationmentioning

confidence: 99%

Protein Microarrays: Novel Developments and Applications

2010

View full text Add to dashboard Cite

Protein microarray technology possesses some of the greatest potential for providing direct information on protein function and potential drug targets. For example, functional protein microarrays are ideal tools suited for the mapping of biological pathways. They can be used to study most major types of interactions and enzymatic activities that take place in biochemical pathways and have been used for the analysis of simultaneous multiple biomolecular interactions involving protein-protein, protein-lipid, protein-DNA and protein-small molecule interactions. Because of this unique ability to analyze many kinds of molecular interactions en masse, the requirement of very small sample amount and the potential to be miniaturized and automated, protein microarrays are extremely well suited for protein profiling, drug discovery, drug target identification and clinical prognosis and diagnosis. The aim of this review is to summarize the most recent developments in the production, applications and analysis of protein microarrays.

show abstract

“…Moreover, the structural sensitivity of proteins calls for chemical transformations that proceed under mild conditions and are compatible with all functional groups present in proteins 1. 2, 12–15 The availability of a method for the fast, oriented, and covalent immobilization of expressed proteins from lysates would be of great value. This approach has been demonstrated previously on the basis of protein transsplicing,16, 17 phosphopantetheinyl transferase catalysis,18 and O 6 ‐alkylguanine‐DNA alkyltransferase (SNAP tag) 19.…”

mentioning

confidence: 99%

Oriented Immobilization of Farnesylated Proteins by the Thiol‐Ene Reaction

Weinrich¹,

Lin²,

Jonkheijm³

et al. 2010

Angewandte Chemie

View full text Add to dashboard Cite

Einfach und unter milden Bedingungen werden Proteine durch Thiol‐En‐Photokupplung zwischen S‐Farnesylgruppen an einer genetisch kodierbaren „CAAX‐Box“‐Tetrapeptidsequenz (A ist aliphatisch) am C‐Terminus des Proteins und Oberflächen‐Thiolen immobilisiert (siehe Schema). Auf diese Art gelingt die gerichtete kovalente Immobilisierung von Proteinen direkt aus Expressionslysaten ohne zusätzliche Reinigungs‐ oder Derivatisierungsschritte.

show abstract

Interfacing "Soft" and "Hard" Matter with Exquisite Chemical Control

Abstract: Abstract. The present paper reviews the recent development of new chemical and biological technologies for the site-specific immobilization of proteins onto inorganic materials and their potential applications to the fields of micro and nanotechnology.

Cited by 12 publications

References 59 publications

Expressed protein ligation: a resourceful tool to study protein structure and function

Expressed protein ligation: a resourceful tool to study protein structure and function

Protein Microarrays: Novel Developments and Applications

Oriented Immobilization of Farnesylated Proteins by the Thiol‐Ene Reaction

Contact Info

Product

Resources

About