Interference in plant defense and development by non-structural protein NSs of Groundnut bud necrosis virus

Goswami, Suneha; Sahana, Nandita; Pandey, Vanita; Doblas, Paula; Jain, Rakesh; Palukaitis, Peter; Canto, Tomás; Praveen, Shelly

doi:10.1016/j.virusres.2011.08.016

Cited by 25 publications

(17 citation statements)

References 26 publications

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“…Thus, it is plausible that a disturbed, miRNA-based regulation contributes to systemic necrosis caused by PVX-PVY infection via oxylipin production. This observation is consistent with a report showing the upregulation of TCP1 and LOX2 in tomato plants infected with Groundnut bud necrosis tospovirus, which might be responsible for the systemic necrosis exhibited by these plants (70).…”

Section: Discussionsupporting

confidence: 93%

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

García-Marcos

Pacheco

Manzano

et al. 2013

J Virol

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One of the most severe symptoms caused by compatible plant-virus interactions is systemic necrosis, which shares common attributes with the hypersensitive response to incompatible pathogens. Although several studies have identified viral symptom determinants responsible for systemic necrosis, mechanistic models of how they contribute to necrosis in infected plants remain scarce. Here, we examined the involvement of different branches of the oxylipin biosynthesis pathway in the systemic necrosis response caused either by the synergistic interaction of Potato virus X with Potato virus Y (PVX-PVY) or by Tomato spotted wilt virus (TSWV) in Nicotiana benthamiana. Silencing either 9-lipoxygenase (LOX), 13-LOX, or ␣-dioxygenase-1 (␣-DOX-1) attenuated the programmed cell death (PCD)-associated symptoms caused by infection with either PVX-PVY or TSWV. In contrast, silencing of the jasmonic acid perception gene, COI1 (Coronatine insensitive 1), expedited cell death during infection with compatible viruses. This correlated with an enhanced expression of oxylipin biosynthesis genes and dioxygenase activity in PVX-PVY-infected plants. Moreover, the Arabidopsis thaliana double lox1 ␣-dox-1 mutant became less susceptible to TSWV infection. We conclude that oxylipin metabolism is a critical component that positively regulates the process of PCD during compatible plant-virus interactions but does not play a role in restraining virus accumulation in planta.

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Section: Discussionsupporting

confidence: 93%

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

García-Marcos

Pacheco

Manzano

et al. 2013

J Virol

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“…CaCV NSs, like other tospoviral NSs proteins (Takeda et al, 2002; Schnettler et al, 2010; Goswami et al, 2012), functions as a suppressor of RNA silencing as evidenced by suppression of sense GFP-induced gene silencing both locally and systemically. Our local silencing suppression data show that GFP fluorescence in leaf patches expressing CaCV NSs remained bright at 7 dpi, whereas GFP expression was silenced in patches expressing TSWV NSs and negative control patches.…”

Section: Discussionmentioning

confidence: 99%

Intracellular Localization, Interactions and Functions of Capsicum Chlorosis Virus Proteins

Gamage

Dietzgen

2017

Front. Microbiol.

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Tospoviruses are among the most devastating viruses of horticultural and field crops. Capsicum chlorosis virus (CaCV) has emerged as an important pathogen of capsicum and tomato in Australia and South-east Asia. Present knowledge about CaCV protein functions in host cells is lacking. We determined intracellular localization and interactions of CaCV proteins by live plant cell imaging to gain insight into the associations of viral proteins during infection. Proteins were transiently expressed as fusions to autofluorescent proteins in leaf epidermal cells of Nicotiana benthamiana and capsicum. All viral proteins localized at least partially in the cell periphery suggestive of cytoplasmic replication and assembly of CaCV. Nucleocapsid (N) and non-structural movement (NSm) proteins localized exclusively in the cell periphery, while non-structural suppressor of silencing (NSs) protein and Gc and Gn glycoproteins accumulated in both the cell periphery and the nucleus. Nuclear localization of CaCV Gn and NSs is unique among tospoviruses. We validated nuclear localization of NSs by immunofluorescence in protoplasts. Bimolecular fluorescence complementation showed self-interactions of CaCV N, NSs and NSm, and heterotypic interactions of N with NSs and Gn. All interactions occurred in the cytoplasm, except NSs self-interaction was exclusively nuclear. Interactions of a tospoviral NSs protein with itself and with N had not been reported previously. Functionally, CaCV NSs showed strong local and systemic RNA silencing suppressor activity and appears to delay short-distance spread of silencing signal. Cell-to-cell movement activity of NSm was demonstrated by trans-complementation of a movement-defective tobamovirus replicon. CaCV NSm localized at plasmodesmata and its transient expression led to the formation of tubular structures that protruded from protoplasts. The D155 residue in the 30K-like movement protein-specific LxD/N50-70G motif of NSm was critical for plasmodesmata localization and movement activity. Compared to other tospoviruses, CaCV proteins have both conserved and unique properties in terms of in planta localization, interactions and protein functions which will effect viral multiplication and movement in host plants.

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“…miR1432 is thought to be involved in pollen development and male sterility ( Yan et al , 2015 ) as well as in drought shock stress ( Kantar et al , 2011 ) in rice. The target gene of miR319, the transcription factor TCP1, is related to the S RNA (NSs) of the Groundnut bud necrosis virus (GBNV) in tomato ( Goswami et al , 2012 ). Leaf-curling symptoms in plants infected with Tomato leaf curl virus (ToLCV) might also be associated with miR319 ( Naqvi et al , 2010 ).…”

Section: Discussionmentioning

confidence: 99%

Dual transcriptome analysis reveals insights into the response toRice black-streaked dwarf virusin maize

Zhou

Duan

et al. 2016

EXBOTJ

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Interference in plant defense and development by non-structural protein NSs of Groundnut bud necrosis virus

Abstract: El artículo seleccionado no se encuentra disponible por ahora a texto completo por no haber sido facilitado todavía por el investigador a cargo del archivo del mismo.

Cited by 25 publications

References 26 publications

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

Oxylipin Biosynthesis Genes Positively Regulate Programmed Cell Death during Compatible Infections with the Synergistic Pair Potato Virus X-Potato Virus Y and Tomato Spotted Wilt Virus

Intracellular Localization, Interactions and Functions of Capsicum Chlorosis Virus Proteins

Dual transcriptome analysis reveals insights into the response toRice black-streaked dwarf virusin maize

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