Interference ofDermatophagoides' Specific Immunoglobulins G in the Quantification of Mite's Specific Immunoglobulins E

Fonseca, Patrícia; Tavares-Ratado, Paulo; Tomaz, Cândida T.

doi:10.1080/15321810903084814

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…This was necessary to minimize the amount of sera required for triplicate measurements of multiple allergenic groups from two concomitant allergen sources. Notoriously, allergen dot immunoblot arrays are generally prone to non‐specific IgE binding as well as non‐specific inhibition of IgE binding by the presence of large quantities of antigen specific IgG in patients sera (34). We did not ascertain the dust mite IgG; however, we did rule out non‐specific IgE binding using ELISA inhibition tests.…”

Section: Discussionmentioning

confidence: 99%

Mite component–specific IgE repertoire and phenotypes of allergic disease in childhood: The tropical perspective

Kidon

Chin

Kang

et al. 2011

Pediatric Allergy Immunology

104

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Sensitization to perennial aeroallergens correlates with the risk of persistent asthma (AS) in children. In tropical Singapore, multiple codominant species of mites abound in the indoor environment, and preferential species-specific sensitization has been associated with different phenotypes of allergic disease. We investigated the pattern of mite component-specific IgE (mcsIgE) in children with different phenotypes of clinical allergic disease in an environment with multiple mite species exposure. A prospective evaluation of newly diagnosed patients with clinical diagnosis of allergic rhinitis (AR), atopic dermatitis (AD), or AS and sensitization to one or more aeroallergens were performed. Sera were tested for specific IgE against an extensive panel of Dermatophagoides pteronyssinus and Blomia tropicalis allergens. A total of 253 children were included, mean age 7.3 yr, 79% fulfilled criteria for AR, 46% AS, 71% AD, and 31% for all three. Sensitization to one or both mites was observed in 91% of children, 89% were sensitized to D. pteronyssinus, and 70% to B. tropicalis. The most common mite allergens recognized by these atopic children were Der p 1 (64%), Der p 2 (71%), Blo t 5 (45%), Blo t 7 (44%), and Blo t 21 (56%). Specific IgE responses to an increased number of distinct mite allergens correlated with the complexity of the allergic phenotype. In multivariate analysis, an increased risk for the multi-systemic phenotype (AR + AS + AD) was associated with sensitization to an increased repertoire of mite components (three or more) (OR 4.3, 95% CI 2.1-8.8, p = 0.001) and a positive parental history of AS (OR 2.4, 95% CI 1.2-2.9, p = 0.013). A highly pleiomorphic IgE response to the prevalent indoor mites is associated with the presence of a multi-systemic allergic phenotype in childhood in a tropical environment.

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Section: Discussionmentioning

confidence: 99%

Mite component–specific IgE repertoire and phenotypes of allergic disease in childhood: The tropical perspective

Kidon

Chin

Kang

et al. 2011

Pediatric Allergy Immunology

104

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“…Chemibeads have a big specific surface area and can provide more binding sites for allergen proteins, including mixed proteins. Consequently, it could reduce the interference of allergen-specific IgG [13], which will appear in the detection of allergen-sIgE [27,28]. In China, the reader of LICA is general equipment and exists in numerous clinical laboratories.…”

Section: Discussionmentioning

confidence: 99%

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of Artemisia-Specific Immunoglobulin E

Liu

Zhang

Zhu

et al. 2021

Int Arch Allergy Immunol

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Background: Light-initiated chemiluminescence assay (LICA) is a homogeneous assay that has been successfully used for the quantitation of food allergen-specific immunoglobulin E (sIgE), but not inhaled allergen-sIgE. Simultaneously, current assays used to detect allergen-sIgE are serum consuming and/or time consuming. Hence, we established a method for the quantitation of Artemisia-sIgE based on LICA and verified its performance according to the clinical guideline documents, laying a foundation for the quantitation of inhaled and food allergen-sIgE in parallel on LICA. Methods: The assay was established after optimizing the first incubation time and the dilutions of Artemisia-coated chemibeads, biotinylated goat anti-human IgE, and serum. In order to quantitate Artemisia-sIgE, the calibration curve was established with a high positive serum of known concentration. The assay performance was confirmed per the clinical guideline documents. In addition, the correlation between the results of LICA and capture enzyme-linked immunosorbent assay was evaluated. Results: The developed LICA’s coefficients of variation of repeatability and intermediate precision were 3.20%, 2.14%, and 3.85% and 4.30%, 4.00%, and 4.40%, respectively. The limit of detection was 0.10 kUA/L, and the limit of quantitation was 0.11 kUA/L. The range of linearity was from 0.27 kUA/L to 97.53 kUA/L (r = 0.9968). The correlation coefficient (r) for the correlation analysis between results of LICA and capture ELISA was 0.9087. This assay was successfully applied in 64 human serum samples, showing good sensitivity (82.20%) and specificity (100%). Conclusion: An Artemisia-sIgE quantitation assay based on LICA was successfully established. Its performance satisfied the clinical requirements and could be widely used in clinical laboratories.

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“…The strongpoint of our study lies in the fact that ImmunoCAP was used to determine specific IgE. Previous studies usually used allergen dot immunoblot arrays that are known to be prone to nonspecific IgE binding as well as nonspecific inhibition of IgE binding by the presence of large quantities of antigen specific IgG in patients sera [ 17 28 ].…”

Section: Discussionmentioning

confidence: 99%

Immunoglobulin E to allergen components of house dust mite in Korean children with allergic disease

Kim

Kang

Won

et al. 2015

Asia Pacific Allergy

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BackgroundHouse dust mites (HDMs) are important sources of indoor allergens. Seventeen components have been identified from Dermatophagoides pteronyssinus (Der p).ObjectiveOur aim was to define the prevalence of specific IgE to components of Der p in Korea and investigate the clinical features of them in children with allergic disease.MethodsWe performed a prospective evaluation of 80 HDM sensitized patients with history of allergic rhinitis (AR), atopic dermatitis (AD), asthma and urticaria (UC). Patients underwent ImmunoCAP for total IgE, Der p, Der f, Der p 1, Der p 2, and Der p 10.ResultsSeventy-nine patients had detectable serum IgE to Der p, 80 patients were sensitized to Der f, 66 patients were sensitized to Der p 1, 63 patients to Der p 2, and 7 patients were sensitized to Der p 10. Der p 1 specific IgE was significantly lower in the UC group compared with the AD and AR group. Total IgE was significantly higher in the Der p 10 sensitized group. Der p 10 serum IgE level was highly correlated with crab and shrimp specific IgE. There was a significant positive correlation between total IgE and specific IgE to Der p and its components and Der f.ConclusionSensitization to HDM and its components in Korea is similar to previous studies from temperate climate. The determination of Der p 1, Der p 2, and Der p 10 specific IgE helps in obtaining additional information in regards to allergic disease.

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Interference ofDermatophagoides' Specific Immunoglobulins G in the Quantification of Mite's Specific Immunoglobulins E

Cited by 4 publications

References 9 publications

Mite component–specific IgE repertoire and phenotypes of allergic disease in childhood: The tropical perspective

Mite component–specific IgE repertoire and phenotypes of allergic disease in childhood: The tropical perspective

A Simple and Rapid Light-Initiated Chemiluminescence Assay for Quantitation of <b><i>Artemisia</i></b>-Specific Immunoglobulin E

Immunoglobulin E to allergen components of house dust mite in Korean children with allergic disease

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