Interference of sodium caseinate in the TBARS assay

PET trays coated with Citrus extract exhibit antioxidant activity with cooked turkey meat

“…Although TBARS values increased over time in meat samples stored on the Citrus extract coated trays, lipid oxidation was almost two fold lower than that of samples on the uncoated trays. The lower TBARS values of meat on the Citrus extract coated trays was shown to be due to an antioxidant effect of the packaging, and not interference of the antioxidant in the TBARS assay as investigated by Caprioli, O'Sullivan, and Monahan (2010) (data not shown).…”

Effect of Fruit and Vegetable Intake on Oxidative Stress and Dyslipidemia Markers in Human and Animal Models

“…It is also used to evaluate oxidation of food systems. TBARS are used to determine malondialdehyde (MDA) and other molecules that are generated as byproducts of lipid oxidation, (it is therefore a measure of oxidative damage) although it can be somewhat unspecific since other compounds also react with thiobarbituric acid (Caprioli et al, 2011).…”

Lipid Oxidation in Cured Meat Model Systems Containing Either Antioxidant or Prooxidant: A Comparative Study on the Determination of Malondialdehyde Concentration by Using Conventional, Test Kit and Chromatographic Assays

“…The most common method to determine MDA concentration is based on the reaction of MDA with 2thiobarbituric acid (TBA) that produces a pink-colored dimeric chromogen complex (Ghani et al 2017;Bertolín et al 2019) which is so-called TBA-reactive substances (TBARS) assay (Caprioli et al 2011).…”

“…Although plenty of modi cations of this assay is still in use today, remarkable disadvantages are attributed to this technique such as the lack of speci city of TBA-MDA reaction and the interference of both TBA and MDA with different food constitutes that could lead to overestimation or erroneously low values (Caprioli et al 2011;Díaz et al 2014;Jung et al 2016), as well as further oxidation risk derived from high temperatures, long incubation periods, and use of aggressive acids (Barriuso et al 2013). From this point of view, the quanti cation of MDA by high-performance liquid chromatography (HPLC) as an advanced method is highly recommended to increase analytical sensitivity and speci city, offering a good solution to avoid such problems mentioned for conventional techniques (Karatas et al 2002; Mendes et al 2009; Papastergiadis et al 2012;Jung et al, 2016).…”