Interference with Multiplication of Pc Phage by a Factor Produced by Exposure of Pseudomonas aeruginosa to Inactivated Pc Phage

Mercer, C. K.; Mills, R. F. N.

doi:10.1099/00221287-23-2-253

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…The present report's finding that there exist basic similarities between fish interferon and other vertebrate interferons on a crude and molecular level supports the belief that the interferon nonimmune system is an ancient antiviral defense system. This contention has been previously supported by reports that the interferon system extends down to the arthropods and bacteria (10,19).…”

Section: Ribonuclease Axmentioning

confidence: 52%

Partial purification and characterization of RTG-2 fish cell interferon

Sena¹,

Rio²

1975

Infect Immun

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Interferon produced by rainbow trout gonadal cells (RTG-2) was partially purified. The physical, chemical, and biological properties of this in vitro produced fish cell interferon were studied. Purification was achieved by ultracentrifugation, molecular sieve gel chromatography, ion exchange chromatography, and polyacrylamide gel electrophoresis. The isoelectric point of RTG-2 interferon, as determined by CM-Sephadex (C-0O) chromatography, was 7.1. Filtration through Sephadex G-150 showed that RTG-2 interferon had a molecular weight of 94,000. The partially purified material was not sedimented at 105,000 x g for 2 h at 4 C. The fish cell interferon was non-dialyzable and exhibited heat and pH stability. The partially purified material was inactivated by treatment with trypsin or 2-mercaptoethanol, but was resistant to treatment with deoxyribonuclease or ribonuclease. RTG-2 interferon which was induced by infectious pancreatic necrosis virus exhibited antiviral activity against challenge with infectious hematopoietic necrosis virus or infectious pancreatic necrosis virus. Partially purified RTG-2 interferon exhibited greater species specificity than the crude material.

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Section: Ribonuclease Axmentioning

confidence: 52%

Partial purification and characterization of RTG-2 fish cell interferon

Sena¹,

Rio²

1975

Infect Immun

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“…The previously collected soil samples were used to detect the lytic phages against soft rot bacterial isolates and strains using spot test and plaque assay techniques according to (Othman, 1997). Lytic phages were isolated and purified using the single plaque isolation method (SPI) as described by Mercer and Mills (1960).…”

Section: Detection and Isolation Of The Lytic Phagesmentioning

confidence: 99%

Controlling Soft Rot Disease of Potatoes Caused by Enterobacter cloacae sub sp. dissolvens via Virulent Bacteriophages

Marei

Embaby

El-Afifi

2020

Journal of Agricultural Chemistry and Biotechnology

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Enterobacter cloaca causes bacterial soft rot disease of potatoes. Bacteriophages can be used as a biocontrol agent against pathogenic bacteria. The possibility of using lytic phage isolates to control soft rot disease of potato was studied.Isolation and identification of different virulent bacteriophages specific to Enterobacter cloacae sub sp. dissolvens was took place by using biological and molecular techniques. The efficiency of lytic phage isolates on E. cloacae sub sp. dissolvens as a biocontrol agent under greenhouse condition was evaluated.The two virulent phage isolates specific to E. cloacae sub sp. dissolvens were isolated, identified and designated as EdIv-1 and EdIv-2. Mixture of the two virulent phages (EdIv-1 and EdIv-2) could be used as a bio control agent against E. cloacae sub sp. dissolvens in soil. The data indicated that the number of bacterium in the soil treated with this treatment as a cocktail of phages were considerably lower than in the soil treated with bacterium only in all the different periods and other treatments. In addition the highest percentage of decreased plants was recorded after 30 days (71%). Virulent phage isolates were found to be stable, infecting E. cloacae sub sp. dissolvens. It could be concluded that cocktail of phages can be used as effective biological control agent against the plant pathogen under the environmental condition in Egypt.

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The Biological Significance of the Interferon System

Baron¹

1970

Arch Intern Med

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Interference with Multiplication of Pc Phage by a Factor Produced by Exposure of Pseudomonas aeruginosa to Inactivated Pc Phage

Cited by 9 publications

References 3 publications

Partial purification and characterization of RTG-2 fish cell interferon

Partial purification and characterization of RTG-2 fish cell interferon

Controlling Soft Rot Disease of Potatoes Caused by Enterobacter cloacae sub sp. dissolvens via Virulent Bacteriophages

The Biological Significance of the Interferon System

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